A generic method for the quantitative analysis of aminoglycosides (and spectinomycin) in animal tissue using methylated internal standards and liquid chromatography tandem mass spectrometry

Holthoon, Frédérique L. van; Essers, M.L.; Mulder, P.; Stead, Sara; Caldow, Marianne; Ashwin, Helen; Sharman, Matthew

doi:10.1016/j.aca.2008.09.026

Cited by 44 publications

(15 citation statements)

References 18 publications

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“…The penicillins were derivatised to their pteridine derivatives, followed by LC-QqQ-MS using ESI(−). The method used for the confirmation of sulphonamides is based on the method described in [15] and the method used for the confirmation of aminoglycosides in [16]. Detection limits were as follows: Sulfonamides 10 g kg −1 ; Tetracyclines 10 g kg −1 ; Flumequine 20 g kg −1 , Ciprofloxacin and Enrofloxacin 10 g kg −1 ; Aivlosin, Tilmicosin and Valnemulin 12.5 g kg −1 ; Tylosin, Tiamulin, Pirlimycin and Tulathromycin 25 g kg −1 ; Erythromycin and Josamycin 50 g kg −1 ; Amoxicillin, Penicillin and Nafcillin 5 g kg −1 ; Ampicillin and Dicloxacillin 10 g kg −1 ; Cloxacillin and Oxacillin 15 g kg −1 .…”

Section: Confirmatory Analysismentioning

confidence: 99%

Comparison of three microbial screening methods for antibiotics using routine monitoring samples

Pikkemaat

Rapallini

Dijk

et al. 2009

Analytica Chimica Acta

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Section: Confirmatory Analysismentioning

confidence: 99%

Comparison of three microbial screening methods for antibiotics using routine monitoring samples

Pikkemaat

Rapallini

Dijk

et al. 2009

Analytica Chimica Acta

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“…Aminoglycosides are analysed in eggs, milk, tissues and fluids of food‐producing animals 17, as well as in human serum 18 and urine 19 for control and monitoring reasons. The chromatographic separation of aminoglycosides can be performed with reverse‐phase columns, using ion‐pairing agents 19–29 or derivatisation agents 30 to achieve suitable retention. However, when MS is used as the detection technique, ion‐pairing reagents can cause ion suppression and contaminate the instrument 31.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic interaction chromatography for the analysis of aminoglycosides

Kumar

Rúbies

Companyó

et al. 2012

J of Separation Science

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The effect of mobile-phase constituents (pH and ionic strength) and chromatographic behaviour of ten aminoglycosides (streptomycin, dihydrostreptomycin, spectinomycin, apramycin, paramomycin, kanamycin A, gentamycin C1, gentamycin C2/C2a, gentamycin C1a and neomycin) in the bare silica, amino, amide and zwitterionic phases of hydrophilic interaction chromatography (HILIC) were studied systematically. Among the stationary phases studied, the zwitterionic phase provided the best separation of aminoglycosides. The effect of pH, ionic concentration and column temperature on retention time, peak shape and sensitivity was studied using a central composite design. pH affected sensitivity of the detection of analytes but not the retention time. High ionic strength in the mobile phase was necessary to control the ionic interactions between ionised aminoglycosides and the hydrophilic phase, thereby influencing peak shape and retention time. Column temperature affected sensitivity of the detection but not the retention time. During method development, crosstalk between the MS/MS channels of the analytes was observed and resolved.

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“…The majority of these, however, either do not detect AMGs or their detection limits are much higher than the MRLs. 17 Alternative screening/confirmation methods based on LC-MS/MS are available, 18 but these approaches are costly to operate when screening many hundreds or thousands of samples. Furthermore, LC-MS/MS methods require highly qualified personnel and cannot currently be used in remote locations with limited technical services.…”

mentioning

confidence: 99%

Toggled RNA Aptamers Against Aminoglycosides Allowing Facile Detection of Antibiotics Using Gold Nanoparticle Assays

Derbyshire¹,

White²,

Bunka³

et al. 2012

Anal. Chem.

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We have used systematic evolution of ligands by exponential enrichment (SELEX) to isolate RNA aptamers against aminoglycoside antibiotics. The SELEX rounds were toggled against four pairs of aminoglycosides with the goal of isolating reagents that recognize conserved structural features. The resulting aptamers bind both of their selection targets with nanomolar affinities. They also bind the less structurally related targets, although they show clear specificity for this class of antibiotics. We show that this lack of aminoglycoside specificity is a common property of aptamers previously selected against single compounds and described as “specific”. Broad target specificity aptamers would be ideal for sensors detecting the entire class of aminoglycosides. We have used ligand-induced aggregation of gold-nanoparticles coated with our aptamers as a rapid and sensitive assay for these compounds. In contrast to DNA aptamers, unmodified RNA aptamers cannot be used as the recognition ligand in this assay, whereas 2′-fluoro-pyrimidine derivatives work reliably. We discuss the possible application of these reagents as sensors for drug residues and the challenges for understanding the structural basis of aminoglycoside-aptamer recognition highlighted by the SELEX results.

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A generic method for the quantitative analysis of aminoglycosides (and spectinomycin) in animal tissue using methylated internal standards and liquid chromatography tandem mass spectrometry

Cited by 44 publications

References 18 publications

Comparison of three microbial screening methods for antibiotics using routine monitoring samples

Comparison of three microbial screening methods for antibiotics using routine monitoring samples

Hydrophilic interaction chromatography for the analysis of aminoglycosides

Toggled RNA Aptamers Against Aminoglycosides Allowing Facile Detection of Antibiotics Using Gold Nanoparticle Assays

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