A genetic analysis of the rose-gespleten region (68C8-69B5) of Drosophila melanogaster.

Cyclin proteins are one of the important components of the mechanism regulating mitosis in eukaryotic cells. We isolated a Drosophila Cyclin‐A mutant in which the progenitor cells of the peripheral nervous system (the sensory mother cells) do not divide properly, causing the loss and other abnormalities of mechanosensory organs in the adult fly. Sequence analysis of the mutant genome reveals that a P element is inserted into the first intron of the Cyclin‐A gene. A 13 kb wild‐type genomic DNA containing the Cyclin‐A transcription units rescued the mutant phenotype when introduced into the mutant fly. The regulation of cell type specific expression of the Cyclin‐A gene is discussed.

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Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster.

Ueda

Togashi

Takahisa

et al. 1992

The EMBO Journal

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Synergistic action of Drosophila cyclins A and B during the G2-M transition.

1993

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A variety of different cyclin proteins have been identified in higher eukaryotes. In the case of cyclin B, functional analyses have clearly demonstrated an important role in the control of entry into mitosis. The function of cyclin A is more complex. It appears to function in the control of both S‐ and M‐phase. The results of our genetic analyses in Drosophila demonstrate that cyclin A has a mitotic function and that it acts synergistically with cyclin B during the G2‐M transition. In double mutant embryos that express neither cyclin A nor cyclin B zygotically, cell cycle progression is blocked just before the exhaustion of the maternally contributed cyclin A and B stores. BrdU‐labeling experiments indicate that cell cycle progression is blocked in G2 before entry into the fifteenth round of mitosis. Expression of either cyclin A or B from heat‐inducible transgenes is sufficient to overcome this cell cycle block. This block is also not observed in single mutant embryos deficient for either cyclin A or B. In cyclin B deficient embryos, cell cycle progression continues after the apparent exhaustion of the maternal contribution, suggesting that cyclin B might not be essential for mitosis. However, mitotic spindles are clearly abnormal and progression through mitosis is delayed in these cyclin B deficient embryos.

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Distinct Mechanisms Triggering Glial Differentiation in Drosophila Thoracic and Abdominal Neuroblasts 6-4

Akiyama-Oda

Hotta

Tsukita

et al. 2000

Developmental Biology

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Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 (NB6-4A) divides symmetrically to produce two glial cells. To understand the relationship between cell division and glia-neuron cell fate determination, we examined and compared the effects of known cell division mutations on the NB6-4T and NB6-4A lineages. Based on observation of expression of glial fate determination and early glial differentiation genes, the onset of glial differentiation occurred in NB6-4A but not in NB6-4T when both cell cycle progression and cytokinesis were genetically arrested. On the other hand, glial differentiation started in both lineages when cytokinesis was blocked with intact cell cycle progression. These results showed that NB6-4T, but not NB6-4A, requires cell cycle progression for acquisition of glial fate, suggesting that distinct mechanisms trigger glial differentiation in the different lineages.

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A genetic analysis of the rose-gespleten region (68C8-69B5) of Drosophila melanogaster.

Cited by 16 publications

References 12 publications

Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster.

Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster.

Synergistic action of Drosophila cyclins A and B during the G2-M transition.

Distinct Mechanisms Triggering Glial Differentiation in Drosophila Thoracic and Abdominal Neuroblasts 6-4

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