A genetic map of Welschriesling × Sirius for the identification of magnesium-deficiency by QTL analysis

Abstract

A genetic linkage map of grapevine was constructed with 92 progeny, which was derived from a cross of Welschriesling × Sirius using the pseudo-testcross strategy. Welschriesling is known for showing low magnesium uptake. The segregation pattern of this defect was compared with the segregation pattern of 251 molecular markers, 237 simple sequence repeat (SSR) and 14 random amplified polymorphic DNA (RAPD) marker. These markers were used to construct the parental genetic maps and could be arranged into 20 linkage groups. The maternal (Welschriesling) and the paternal (Sirius) maps were aligned in one consensus map. Mapmaker 3.0 software was used for the calculations of linkage groups. The genetic map rendered it possible to identify a QTL (quantitative trait loci) in linkage group 11. This loci can be associated with visual symptoms of Mg lack and the amount of Mg concentration in the leaves of the grape zone. The SSR markers VVS2 and VMC 6g1 showed the highest linkage to the core region of the Mg-QTL.Abbreviations: AFLP, amplified fragment length polymorphism; LOD, likelihood odds ratio; QTL, quantitative trait loci; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; SSR, simple sequence repeat

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

“…This chromosome appears to be problematic as Lowe and Walker (2006) also could not construct a linkage group 15 for 'Riparia Gloire' (V. riparia), while the map for the other parent, 'Ramsey' (V. champinii), contained four markers spanning a region of 28 cM. Similarly, the map for 'Sirius' (an inter-species hybrid descendant from a cross of 'Bacchus' 9 'Villard Blanc') was absent, despite the fact that the consensus map contained eight markers (Mandl et al 2006). A map could also not be calculated for chromosome 15 in the 'VRH 3082 1-42' 9 'SK77 5/3' population investigated by Moreira et al (2011).…”

A linkage map for flowering dogwood (Cornus florida L.) based on microsatellite markers

“…Due to high heterozygousity in out-breeding species, an F 1 population pseudo-testcross strategy has been widely used for linkage analysis (Grattapaglia and SederoV 1994;Arcade et al 2000;Porceddu et al 2002;Hanley et al 2002;Barcaccia et al 2003;La Rosa et al 2003;DoucleV et al 2004;Dirlewanger et al 2004;Graham et al 2004;Beedanagari et al 2005;Kenis and Keulemans 2005;Verde et al 2005;Lanteri et al 2006;Lowe and Walker 2006;Mandl et al 2006). Other mapping populations such as F 1 full-sib progeny (Maliepaard et al 1997;Venkateswarlu et al 2006) and BC 1 mapping populations (Lalli et al 2008) have also been used for linkage map construction.…”

“…Other mapping populations such as F 1 full-sib progeny (Maliepaard et al 1997;Venkateswarlu et al 2006) and BC 1 mapping populations (Lalli et al 2008) have also been used for linkage map construction. In a pseudo-testcross, a single full-sib population is generated by crossing two parents in which one parent is heterozygous at one locus, and the other parent is homozygous at other loci (Mandl et al 2006). Although the pseudo-testcross strategy has been widely used in the mapping of many out-breeding species, it is diYcult to determine if one of the parents is homozygous for any speciWc locus or loci.…”

Breeding of fungal resistant varieties derived from Grüner Veltliner by chromosomal selection

“…For Malverina the definition of chromosome 11 failed due to homozygous alleles. In the same group the QTL for Mg uptake was detected [17].…”