A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

Phulwaria, Mahendra; Manoj, K.; Patel, Ashok Kumar; Kataria, Vinod; Shekhawat, N. S.

doi:10.1093/aobpla/pls054

Cited by 29 publications

(12 citation statements)

References 29 publications

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“…Celastrus paniculatus (Phulwaria et al 2013) and Morinda coreia (Shekhawat et al 2015a). It was observed that nodal segments with one node died due to the effect of HgCl 2 during surface sterilization but the middle nodes were survived even after HgCl 2 treatment when the nodal explants were used with 2-3 nodes.…”

Section: Resultsmentioning

confidence: 99%

“…The maximum number of shoots (8.2 ± 0.17 shoots/culture) were produced when the mother explants were subcultured on fresh medium (Table. 3). Shoot multiplication during repeated transfer may be due to inhibition of apical dominance which stimulates basal dormant meristematic cells to produce young shoots (Phulwaria et al 2013). This approach for shoot multiplication has been used in several plant species (Panwar et al 2012).…”

Section: Multiplication Of Shoots In Culturesmentioning

confidence: 99%

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In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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Section: Resultsmentioning

confidence: 99%

Section: Multiplication Of Shoots In Culturesmentioning

confidence: 99%

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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“…Every species require optimum pH which promote maximum shoot formation. Molecular markers, mainly RAPD, have been widely used for testing the genetic fidelity among in vitro regenerated plantlets in a number of plant species (Savita et al 2012;Phulwaria et al 2012) owing to their simplicity, cost-effectiveness and lower DNA requirement for analysis.…”

Section: Discussionmentioning

confidence: 99%

“…may lead to genomic changes in the in vitro raised plantlets (Jain 2001) Thus, analysis of the genetic homogeneity of tissue culture raised plants is a prerequisite for their exploitation on a commercial scale . DNA-based molecular markers are useful tools for analysis of the genetic fidelity of micropropagated plantlets (Phulwaria et al 2012;Yadav et al 2013). Limited number of shoots (3.0) regeneration have been reported earlier from seedling derived explants e.g., node (Rajasubramaniam & Pardha Saradhi 1994) and hypocotyl calli (Rajasubramaniam & Pardha Saradhi 1997) of P. fraternus.…”

Section: Introductionmentioning

confidence: 99%

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

et al. 2014

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Phyllanthus fraternus is an important medicinal plant, popularly known for its hepatoprotective and antiviral activities since ancient times. Various physiological factors like carbon sources, concentration of agar, pH of the media and effect of season of explants collection were optimized for high frequency regeneration of P. fraternus. The frequency of regeneration, average number and length of shoots were highly influenced by the type and concentration of carbon sources (monosaccharides and disaccharides, 1 to 4%), agar concentration (0.2 to 1%) and pH (4.5 to 6.8) of the media. Media containing 3% sucrose, 0.6% agar at pH 5.8 was best for regeneration. Seasonal variation of explants collection significantly affected the axillary shoots proliferation from explants and best proliferation was observed from explants collected during April to June. Genetic fidelity of regenerated plants was assessed by random amplified polymorphic DNA markers. No polymorphism was detected in micropropagated plants and the mother plant, revealing the genetic homogeneity of the in vitro raised plantlets. This is the first report regarding establishment of genetic fidelity of micropropagated P. fraternus plants, which could be successfully applied for the mass multiplication, germplasm conservation and further genetic transformation assays to meet the ever increasing demand of this medicinally potent plant for industrial and pharmaceutical uses.

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“…Highest percent of bud break and multiple shoots (4.3 ± 0.5) was obtained in MS medium + BA (1.0 mg/L), and 100 % rooting was achieved in half-strength MS medium supplemented with 0.5 mg/L NAA which was followed by acclimatisation in sterile soil and sand (3:1) giving 70 % survival rate. However, the most elaborated, rapid and successful protocol has been described by Phulwaria et al ( 2013 ).…”

Section: Celastrus Paniculatus Willdmentioning

confidence: 99%

Micropropagation: A Boon for Conservation of Valuable Vines and Lianas

Sharma

Shahzad

Akhtar

et al. 2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Woody climbers or lianas are one of the important assets of nature upon which rely many folk and traditional systems of medicine. The increasing need and demand with growing population and thoughtless utilisation of natural products, so far, have resulted into a dwindling population of many plant species. In the present scenario, increasing health issues, hazardous effects of synthetic drugs and thus the preference of natural products over synthetic one, is questioning the survival of many valuable medicinal plant species. Moreover, their own growth pattern, reproductive behaviour and maturation time are the key issues for which fast alternative propagation methods are sought. Advances in the fi eld of biotechnology have opened fresh avenues for replenishment and conservation of such high value plantsplant tissue culture (PTC) or in vitro technique is one among them. In vitro techniques like adventitious shoot regeneration, somatic embryogenesis, bioreactors and fermentation process have speed up regeneration time manyfold. The techniques like protoplast culture, micrografting and genetic transformation have been implied for variety improvement, whereas cryopreservation, slow growth methods and synseed production have been reported for long-term storage of germplasm and their exchange. Thus, PTC justifi es a great deal of contribution in conserving the wealth of nature proving itself a reliable technique in plant biodiversity conservation. Nowadays laudable attempts have been done for the conservation of various useful medicinal climbers through PTC such as Salacia

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A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

Cited by 29 publications

References 29 publications

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

Micropropagation: A Boon for Conservation of Valuable Vines and Lianas

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