A  GENOME-WIDE  ANALYSIS  OF  THE GALACTINOL  SYNTHASEGENE  FAMILY  IN  BANANA (Musa acuminata)

Dolcimasculo, Fernanda; Ribas, Alessandra Ferreira; Vieira, Luiz Gonzaga Esteves; Santos, Tiago Benedito dos

doi:10.5747/ca.2018.v14.n3.a222

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…Subcellular localization predictions for ItlbGolS and ItfGolS suggest that diversity is found in the cell wall, chloroplast, cytoplasm, mitochondrion, peroxisome, cell membrane, and nucleus (Table Supplementary 1). As previously reported, some subcellular locations are in the same membrane tissue (Santos et al, 2011;Fan et al, 2017;Dolcimasculo et al, 2018).…”

Section: Resultssupporting

confidence: 75%

“…Based on this valuable information, it can be suggested that in sweet potato, this gene presents less diversity in its structure. In Musa acuminata, the exon/intron of the GolS genes shows three or four exons (Dolcimasculo et al, 2018). According to Figures 1 and 2, there may be a variation in the number of exons and introns in the ItlbGolS and ItfGolS genes, and we can also suggest that some genes are duplicated.…”

Section: Resultsmentioning

confidence: 89%

“…GolS gene has been identified and characterized in different plant species, both in monocot and dicot: Arabidopsis thaliana (Nishizawa et al, 2008), Coffea arabica L. and C. canephora (Santos et al, 2011;, Pisum sativum (Lahuta et al, 2014), Solanum lycopersicum and Brachypodium distachyon (Filiz et al, 2015), Manihot esculenta Crantz (Li et al, 2018), Musa acuminata (Dolcimasculo et al, 2018), Panicum virgatum L. and P. hallii (Góis et al, 2020), Phaseolus vulgaris L. (Koning et al, 2023). Plants have more than one isoform of GolS and can act on specific tissues (Sengupta et al, 2012;revised by Santos;Vieira, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Galactinol Synthase (Gols) Genes in Ipomoea Triloba and I. Trifida: An Approach Using Bioinformatics

Rufino,

Lobo,

Yaguinuma

et al. 2024

COLLOQ AGRARIAE

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Galactinol synthase (GolS - EC 2.4.1.123) is classified as a key enzyme that catalyzes the first step in the synthesis pathway of the raffinose family (RFOs). Although GolS genes have been characterized in several important species, their characterization in sweet potatoes has yet to be explored. Ipomoea trifida (Kunth) G. Don (2n = 2x = 30) is currently described as one of the closest ancestors of the sweet potato and considered an excellent crossbreeding species, allowing the introgression of important genes such as GolS. This study aimed to identify and characterize in silico the GolS genes in I. trifida and compare with I. triloba. We identified nine GolS genes, five in I. triloba and four in I. trifida. Our study encompassed various aspects, including gene structure analysis, motif identification, chromosomal distribution, synteny analysis, and gene expression. The presence of gene duplications and purifying selection were highlighted, suggesting the evolutionary significance of GolS genes in these species. Phylogenetic analysis categorized GolS proteins into three groups, potentially reflecting distinct functional roles. Furthermore, synteny analysis revealed orthologous relationships between GolS genes in the studied species and related plants, contributing to our understanding of their evolutionary history. In silico expression analysis across diverse tissues unveiled tissue-specific expression patterns, hinting at specialized roles for GolS genes in different plant organs. These findings contribute to the broader field of plant genetics, carbohydrate metabolism, and agriculture, offering opportunities for crop improvement and sustainable food production.

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Characterization of Galactinol Synthase (Gols) Genes in Ipomoea Triloba and I. Trifida: An Approach Using Bioinformatics

Rufino,

Lobo,

Yaguinuma

et al. 2024

COLLOQ AGRARIAE

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show abstract

“…Galactitol and raffinose synthase enzymes are critical for raffinose synthesis [ 5 ]. To date, there were a few reports regarding GolS and RFS genes in horticultural crops, such as cucumber [ 40 ], peas [ 41 ], apple [ 12 ], banana [ 42 ], tea [ 7 ] and grapevine [ 24 ]. Kiwifruit is an important horticultural crop with enriching in vitamin C [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiling Analysis of Kiwifruit GolS and RFS Genes and Identification of AcRFS4 Function in Raffinose Accumulation

Yang

Ling

Liu

et al. 2022

IJMS

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The raffinose synthetase (RFS) and galactinol synthase (GolS) are two critical enzymes for raffinose biosynthesis, which play an important role in modulating plant growth and in response to a variety of biotic or abiotic stresses. Here, we comprehensively analyzed the RFS and GolS gene families and their involvement in abiotic and biotic stresses responses at the genome-wide scale in kiwifruit. A total of 22 GolS and 24 RFS genes were identified in Actinidia chinensis and Actinidia eriantha genomes. Phylogenetic analysis showed that the GolS and RFS genes were clustered into four and six groups, respectively. Transcriptomic analysis revealed that abiotic stresses strongly induced some crucial genes members including AcGolS1/2/4/8 and AcRFS2/4/8/11 and their expression levels were further confirmed by qRT-PCR. The GUS staining of AcRFS4Pro::GUS transgenic plants revealed that the transcriptionlevel of AcRFS4 was significantly increased by salt stress. Overexpression of AcRFS4 in Arabidopsis demonstrated that this gene enhanced the raffinose accumulation and the tolerance to salt stress. The co-expression networks analysis of hub transcription factors targeting key AcRFS4 genes indicated that there was a strong correlation between AcNAC30 and AcRFS4 expression under salt stress. Furthermore, the yeast one-hybrid assays showed that AcNAC30 could bind the AcRFS4 promoter directly. These results may provide insights into the evolutionary and functional mechanisms of GolS and RFS genes in kiwifruit.

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Involvement of the galactinol synthase gene in abiotic and biotic stress responses: A review on current knowledge

Santos

Vieira

2020

Plant Gene

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A GENOME-WIDE ANALYSIS OF THE GALACTINOL SYNTHASEGENE FAMILY IN BANANA (Musa acuminata)

Cited by 4 publications

References 29 publications

Characterization of Galactinol Synthase (Gols) Genes in Ipomoea Triloba and I. Trifida: An Approach Using Bioinformatics

Characterization of Galactinol Synthase (Gols) Genes in Ipomoea Triloba and I. Trifida: An Approach Using Bioinformatics

Genome-Wide Expression Profiling Analysis of Kiwifruit GolS and RFS Genes and Identification of AcRFS4 Function in Raffinose Accumulation

Involvement of the galactinol synthase gene in abiotic and biotic stress responses: A review on current knowledge

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