A Genome-Wide Screen Identifies Genes in Rhizosphere-Associated
            <i>Pseudomonas</i>
            Required to Evade Plant Defenses

Liu, Zhexian; Beskrovnaya, Polina; Melnyk, Ryan A.; Hossain, Sarzana S.; Khorasani, Sophie; O'Sullivan, Lucy; Wiesmann, Christina L.; Bush, Jen; Richard, Joël D; Haney, Cara H.

doi:10.1128/mbio.00433-18

Cited by 103 publications

(100 citation statements)

References 62 publications

(100 reference statements)

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“…Inoculation of wild type and immunocompromised Arabidopsis plants with Pseudomonas sp. WC365 identified 231 genes involved in rhizosphere competence (Liu et al, 2018). Follow-up experiments with targeted single gene knockout mutants confirmed the role of the mutants spuC and morA in inducing pattern-triggered immunity of the Arabidopsis host and inhibiting plant growth.…”

Section: Rb-tnseqmentioning

confidence: 92%

“…Rice, maize and sorghum Tn-Seq Constructed transposon insertion libraries -identified 395 genes essential for growth in rich media for leaf, root and rhizosphere colonization (Cole et al, 2017;Liu et al, 2018;Helmann et al, 2019a;Sivakumar et al, 2019). An RB-TnSeq study of P. syringae found 31 genes important for colonization of common bean leaf surfaces, including amino acid and polysaccharide biosynthesis genes.…”

Section: Rb-tnseqmentioning

confidence: 99%

“…Follow-up experiments with targeted single gene knockout mutants confirmed the role of the mutants spuC and morA in inducing pattern-triggered immunity of the Arabidopsis host and inhibiting plant growth. Point mutations in the conserved GGDEF motif of MorA showed that this protein acts as a phosphodiesterase which inhibits biofilm formation (Liu et al, 2018).…”

Section: Rb-tnseqmentioning

confidence: 99%

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Application of Transposon Insertion Sequencing to Agricultural Science

2020

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Many plant-associated bacteria have the ability to positively affect plant growth and there is growing interest in utilizing such bacteria in agricultural settings to reduce reliance on pesticides and fertilizers. However, our capacity to utilize microbes in this way is currently limited due to patchy understanding of bacterial-plant interactions at a molecular level. Traditional methods of studying molecular interactions have sought to characterize the function of one gene at a time, but the slow pace of this work means the functions of the vast majority of bacterial genes remain unknown or poorly understood. New approaches to improve and speed up investigations into the functions of bacterial genes in agricultural systems will facilitate efforts to optimize microbial communities and develop microbe-based products. Techniques enabling high-throughput gene functional analysis, such as transposon insertion sequencing analyses, have great potential to be widely applied to determine key aspects of plant-bacterial interactions. Transposon insertion sequencing combines saturation transposon mutagenesis and high-throughput sequencing to simultaneously investigate the function of all the nonessential genes in a bacterial genome. This technique can be used for both in vitro and in vivo studies to identify genes involved in microbe-plant interactions, stress tolerance and pathogen virulence. The information provided by such investigations will rapidly accelerate the rate of bacterial gene functional determination and provide insights into the genes and pathways that underlie biotic interactions, metabolism, and survival of agriculturally relevant bacteria. This knowledge could be used to select the most appropriate plant growth promoting bacteria for a specific set of conditions, formulating crop inoculants, or developing crop protection products. This review provides an overview of transposon insertion sequencing, outlines how this approach has been applied to study plant-associated bacteria, and proposes new applications of these techniques for the benefit of agriculture.

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Section: Rb-tnseqmentioning

confidence: 92%

Section: Rb-tnseqmentioning

confidence: 99%

Section: Rb-tnseqmentioning

confidence: 99%

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Application of Transposon Insertion Sequencing to Agricultural Science

2020

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“…Although some recent publications about amino acid catabolism in pseudomonads have indicated the role of these catabolic processes in the lifestyle of Pseudomonas (Zhang et al ., , ; Thompson et al ., ,b), scarce information about the implications of BA catabolism in the behaviour and fitness of pseudomonads is actually available. However, putrescine catabolism has been indicated by Liu and colleagues () as an important determinant of Pseudomonas sp. WCS365 fitness when colonizing the rhizosphere.…”

Section: Catabolic Potential Of Pseudomonas Speciesmentioning

confidence: 99%

Catabolism of biogenic amines in Pseudomonas species

Luengo

Olivera

2020

Environmental Microbiology

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Summary Biogenic amines (BAs; 2‐phenylethylamine, tyramine, dopamine, epinephrine, norepinephrine, octopamine, histamine, tryptamine, serotonin, agmatine, cadaverine, putrescine, spermidine, spermine and certain aliphatic amines) are widely distributed organic molecules that play basic physiological functions in animals, plants and microorganisms. Pseudomonas species can grow in media containing different BAs as carbon and energy sources, a reason why these bacteria are excellent models for studying such catabolic pathways. In this review, we analyse most of the routes used by different species of Pseudomonas (P. putida, P. aeruginosa, P. entomophila and P. fluorescens) to degrade BAs. Analysis of these pathways has led to the identification of a huge number of genes, catabolic enzymes, transport systems and regulators, as well as to understanding of their hierarchy and functional evolution. Knowledge of these pathways has allowed the design and collection of genetically manipulated microbes useful for eliminating BAs from different sources, highlighting the biotechnological applications of these studies.

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“…This enzyme functions as a c-di-GMP phosphodiesterase to repress P. fluorescens biofilm formation, which is a strong trigger of plant immune responses. Polyamines, such as putrescine, may serve as plant-derived signals that trigger biofilm formation and must be actively sensed and countered by bacteria (83).…”

Section: Outside the Test Tube: Microbial Chemotaxis In The Natural Wmentioning

confidence: 99%

New Twists and Turns in Bacterial Locomotion and Signal Transduction

et al. 2019

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Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular organisms and within complex ecosystems. Advances in our understanding of the one- and two-component signal transduction systems that underlie these sensing pathways have been driven by advances in imaging the behavior of many individual bacterial cells, as well as visualizing individual proteins and protein arrays within living cells. Cryo-electron tomography continues to provide new insights into the structure and function of chemosensory receptors and flagellar motors, while advances in protein labeling and tracking are applied to understand information flow between receptor and motor. Sophisticated microfluidics allow simultaneous analysis of the behavior of thousands of individual cells, increasing our understanding of how variance between individuals is generated, regulated, and employed to maximize fitness of a population. In vitro experiments have been complemented by the study of signal transduction and motility in complex in vivo models, allowing investigators to directly address the contribution of motility, chemotaxis, and aggregation/adhesion on virulence during infection. Finally, systems biology approaches have demonstrated previously uncharted areas of protein space in which novel two-component signal transduction pathways can be designed and constructed de novo. These exciting experimental advances were just some of the many novel findings presented at the 15th Bacterial Locomotion and Signal Transduction conference (BLAST XV) in January 2019.

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A Genome-Wide Screen Identifies Genes in Rhizosphere-Associated Pseudomonas Required to Evade Plant Defenses

Cited by 103 publications

References 62 publications

Application of Transposon Insertion Sequencing to Agricultural Science

Application of Transposon Insertion Sequencing to Agricultural Science

Catabolism of biogenic amines in Pseudomonas species

New Twists and Turns in Bacterial Locomotion and Signal Transduction

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