A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Abstract

The main aim of the work is to study the regulation of gene expression in the interaction between rice and Magnaporthe oryzae by gene chip technology. In this study, we mainly focused on changes of gene expression at 24, 48, and 72 hours post-inoculation (hpi), through which we could conduct a more comprehensive analysis of rice blast-related genes in the process of infection. The results showed that the experimental groups contained 460, 1227, and 3937 significant differentially expressed genes at 24, 48, and 72 hpi, respectively. Furthermore, 115 significantly differentially expressed genes were identified in response to rice blast infection at all three time points. By annotating these 115 genes, they were divided into three categories: metabolic pathways, proteins or enzymes, and organelle components. As expected, many of these genes were known rice blast-related genes; however, we discovered new genes with high fold changes. Most of them encoded conserved hypothetical proteins, and some were hypothetically conserved genes. Our study may contribute to finding new resistance genes and understanding the mechanism of rice blast development.

Complete genome sequence of the Clostridium difficile LCL126

“…But no reports related to serious diseases found till now. Previous study described the number and type of the pro-inflammatory strain surface protein SlpA that may relate to toxic behavior in C. difficile [ 14 , 36 , 37 ]. The genomic information was provided in present study confirmed that C. difficile LCL126 has some relevant toxin genes, while in-depth study of its metabolome and functional genes needs to be further studied to explore the toxicological mechanism of C. difficile LCL126, aimed to improve the comprehensive understanding of microorganisms and promote the C. difficile related infectious diseases.…”