A genosensor for detection of consensus DNA sequence of Dengue virus using ZnO/Pt-Pd nanocomposites

Singhal, Chaitali; Pundir, C.S.; Narang, Jagriti

doi:10.1016/j.bios.2017.05.047

Cited by 106 publications

(52 citation statements)

References 23 publications

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“…These include East-Central South African (ECSA), West African, Asian, and the Indian Ocean Lineage (IOL) 3,4 . This disease transmits to the humans by infected mosquitoes, namely; Aedes agypti and Aedes albopictis 5 . CHIG has been characterized by onset of sudden fever followed by skin rashes, rigorous joint pain and relentless rheumatic symptoms 1,6 .…”

Section: Introductionmentioning

confidence: 99%

“…Advancements in electrochemical biosensors have motivated various designs of real time POC diagnosis tools 10 . The advantages such as rapid response time, low cost and suitability for mass production associated with detection of DNA hybridization have triggered the development of DNA-based electrochemical biosensors 5,11,12 . These advantages motivated the present work wherein we have developed an electrochemical DNA biosensor for the detection of CHIGV DNA.…”

Section: Introductionmentioning

confidence: 99%

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Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Singhal

Khanuja

Chaudhary

et al. 2018

Sci Rep

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100

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Development of platforms for a reliable, rapid, sensitive and selective detection of chikungunya virus (CHIGV) is the need of the hour in developing countries. To the best of our knowledge, there are no reports available for the electrochemical detection of CHIGVDNA. Therefore, we aim at developing a biosensor based on molybdenum disulphide nanosheets (MoS2 NSs) for the point-of-care diagnosis of CHIGV. Briefly, MoS2 NSs were synthesized by chemical route and characterized using scanning electron microscopy, transmission electron microscopy, UV-Vis spectroscopy, Raman spectroscopy and X-Ray Diffraction. MoS2 NSs were then subjected to physical adsorption onto the screen printed gold electrodes (SPGEs) and then employed for the detection of CHIGV DNA using electrochemical voltammetric techniques. Herein, the role of MoS2 NSs is to provide biocompatibility to the biological recognition element on the surface of the screen printed electrodes. The detection strategy employed herein is the ability of methylene blue to interact differentially with the guanine bases of the single and double-stranded DNA which leads to change in the magnitude of the voltammetric signal. The proposed genosensor exhibited a wide linear range of 0.1 nM to 100 µM towards the chikungunya virus DNA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Singhal

Khanuja

Chaudhary

et al. 2018

Sci Rep

Self Cite

100

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“…Conventional diagnostic assays employed for dengue detection such as isolation of the virus, detection of DENV specific antibodies, ELISA, and PCR have some limitations. 4 They reported that the coupling of smart nanomaterials as ZnO/Pt-Pb nanocomposite in electrochemical genosensors improve the immobilization and sensitivity to virus DNA target, with LOD of 4.3x10 -5 M. In Hepatitis virus which is a global health problem, Honorato Castro et al 23 established for the first time an electrochemical genosensor incorporating a specific oligonucleotides probe detecting Hepatitis B virus, by direct (detect hybridization by direct oxidation of guanosine and adenosine base) or indirect (using ethidium bromide as a hybridization indicator) electrochemical transduction, based on a graphite electrode modified with a poly(4aminophenol) matrix. This efficiency of the use of nanocomplexes has also been reported by Rolim et al 24 whose used electrochemical sandwich-type genosensors based upon DNA-AuNPs for detection of a Systemic Arterial Hypertension (SAH) polymorphism located in intron 16 of the Angiotensin-converter enzyme (ACE) gene (related to heart diseases, especially blood hypertension, its early detection is of great biomedical interest).…”

Section: Plants Breeding Food Safety and Quality Controlmentioning

confidence: 99%

“…The electrochemical DNA-based genosensors can be coupled with nanoparticles or nanocomposites to improve both oligonucleotides sequence immobilization on the transducer surface and sensitivity to hybridization. [3][4][5][6][7] The electrochemical techniques applied in genosensor can be used in different goals, e.g Differential Pulse Voltammetry (DPV) as analystical technique is usually used to measure the concentration of some specific electroactive species with high sensitivity. 8 This paper is devoted to giving a general idea about the fields of application of electrochemistry, such as medicine, plant breeding, food safety and quality control, and bacterial and viral analysis.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Genosensors: Definition and Fields of Application

Goumi

2017

IJBSBE

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“…We report herein the application of electrochemical Paper-based analytical devices (ePADs) conjugated with graphitic carbon nitride and DNA probe for the detection of norovirus DNA. DNA Probe employed in the fabrication of the biosensor was the consensus sequence as NoV can be classified as five different distinct geno-groups in order to determine the DNA sequence of all distinct genotypes [29]. The fabricated biosensor showed a low response time, high specificity and selectivity with a low detection limit.…”

mentioning

confidence: 99%

Graphitic Carbon Nitride as an Amplification Platform on an Electrochemical Paper-Based Device for the Detection of Norovirus-Specific DNA

Rana

Killa

Yadav

et al. 2020

Sensors

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Norovirus is one of the leading causes of gastroenteritis, acute vomiting, intense diarrhoea, acute pain in the stomach, high fever, headaches, and body pain. Conventional methods of detection gave us very promising results but had disadvantages such as low sensitivity, cost ineffectiveness, reduced specificity and selectivity, etc. Therefore, biosensors can be a viable alternative device which can overcome all setbacks associated with the conventional method. An electrochemical sensor based on oxidized graphitic carbon nitride (Ox-g-C 3 N 4 ) modified electrochemical paper-based analytical device (ePAD) was fabricated for the detection of norovirus DNA. The synthesized Ox-g-C 3 N 4 nanosheets were characterized by field emission scanning electron microscopy (FESEM), X-ray Diffraction (XRD), UV-Vis spectroscopy and X-Ray Photoelectron Spectroscopy. The capture probe DNA (PDNA) modified electrodes were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). These two characterization techniques were also employed to find the optimal scan rate, response time and temperature of the fabricated sensor. The fabricated biosensor showed a limit of detection (LOD) of 100 fM. Furthermore, the specificity of the reported biosensor was affirmed by testing the response of capture probe DNA with oxidized graphitic carbon nitride (PDNA/Ox-g-C 3 N 4 ) modified ePAD on the introduction of a non-complimentary DNA. The fabricated ePAD sensor is easy to fabricate, cost effective and specific, and requires a minimum analysis time of 5 s. of 11subsequently, need to be hospitalized and around 570-800 of these people die annually [3][4][5]. Food virology research towards the detection of human norovirus has increased manifold after recognizing norovirus as a prominent contributor towards foodborne epidemics [6][7][8]. Conventional methods such as electron microscopy (EM) [9], reverse transcriptase PCR (RT-PCR) assays [10], and enzyme immunoassays (EIAs) [11] were used for detection of the norovirus. These conventional methods gave us very promising results but had their disadvantages such as low sensitivity, cost ineffectiveness, need for experts or professionals to operate the systems, reduced specificity and selectivity [3,12,13]. Hence, biosensors can overcome the shortcomings of the previously used methods of detection such as specificity, sensitivity, and portability [14]. Among them, DNA-based electrochemical biosensors are receiving a lot of attention due to the high specificity of the DNA. Integration of nanomaterials further amplify the response signal which enhances the sensitivity and selectivity of the biosensor [15][16][17]. Biosensors integrated with nanomaterials have shown better stability as well as chemical and electrical properties [18]. Here, graphitic carbon nitride (g-C 3 N 4 ) particles were chosen for the interface of the paper electrodes. g-C 3 N 4 is a semiconducting material which is analogous to graphene with stacked two-dimensional structures [19,20]. The semiconducting 2-D g-C 3 N...

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A genosensor for detection of consensus DNA sequence of Dengue virus using ZnO/Pt-Pd nanocomposites

Cited by 106 publications

References 23 publications

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Electrochemical Genosensors: Definition and Fields of Application

Graphitic Carbon Nitride as an Amplification Platform on an Electrochemical Paper-Based Device for the Detection of Norovirus-Specific DNA

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