A global metabolomic insight into the oxidative stress and membrane damage of copper oxide nanoparticles and microparticles on microalga Chlorella vulgaris

Wang, Lei; Huang, Xulei; Sun, Weiqing; Too, Hui Zhen; Laserna, Anna Karen Carrasco; Li, Sam Fong Yau

doi:10.1016/j.envpol.2019.113647

Cited by 41 publications

(19 citation statements)

References 51 publications

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“…Phosphoglycerides, including lecithin (PC), cephalin (PE), and phosphatidylserine (PS), are important components of the cell membrane and form the basis of cell metabolism, energy metabolism, and signal transmission. Lysophospholipids, the raw material of biomembranes, also participate in the biological processes of most cells ( Schulte, 2015 ; Wang et al, 2020 ). After Axn feeding, the levels of almost all PEs, PCs, PSs, and LysoPCs decreased.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, Transcriptome and the Metabolome Response to Dietary Astaxanthin in Exopalaemon carinicauda

Wang

et al. 2022

Front. Physiol.

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Astaxanthin (Axn), a feed additive, is becoming increasingly important for modulating the metabolism, growth, development, and reproduction of aquatic organisms in aquaculture. In this study, Exopalaemon carinicauda (E. carinicauda) is an economically important fishery species in China that has been found to exhibit increased body weight following Axn feeding as compared to a standard diet. The antioxidant, transcriptomic, and metabolomic analyses of the response of E. carinicauda after Axn feeding were investigated. Axn could reduce the content of malondialdehyde and increase the activities of various antioxidant enzymes, which also proved that axn can improve the antioxidant capacity Transcriptomic analysis suggested that synthesis and secretion of immune proteins, cytoskeleton structure, and apoptosis signaling were altered after Axn feeding. The metabolic response to axn mainly includes the up regulation of different amino acids and the change of unsaturated fatty acids. Combined transcriptomic and metabolomic data indicated that amino acid metabolic pathways were upregulated in the muscles after Axn feeding. For good measure, energy metabolism pathways were upregulated in the muscles to improve ATP and unsaturated fatty acid production. This study provides key information to increase our understanding of the effects of Axn in shrimp.

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Section: Discussionmentioning

confidence: 99%

Antioxidant, Transcriptome and the Metabolome Response to Dietary Astaxanthin in Exopalaemon carinicauda

Wang

et al. 2022

Front. Physiol.

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“…It was also reported that aspartic acid and proline in Chlorella vulgaris were significantly upregulated after exposure to CuO NPs. 48 Tyrosine is an aromatic amino acid derived from the shikimic acid pathway. It is used in protein synthesis and is also a precursor of many natural products, such as alkaloids, hormones, and cell wall components.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…A recent study also found that the membrane lipids of microalgae cells remodeled after exposure to copper-based materials. 48 Other Metabolites. Other important metabolites that are not covered in the above discussions include (9Z,11E,15Z)-(13S)-hydroxyoctadeca-9,11,15-trienoate (13(S)-HOTrE), arachidic acid, and linoleic acid (Figure 4).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Transcriptomics and Metabolomics Revealed the Biological Response of Chlorella pyrenoidesa to Single and Repeated Exposures of AgNPs at Different Concentrations

Cao

Huang

Wang

et al. 2021

Environ. Sci. Technol.

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Increased release of engineered nanoparticles (ENPs) from widely used commercial products has threatened environmental health and safety, particularly the repeated exposures to ENPs with relatively low concentration. Herein, we studied the response of Chlorella pyrenoidesa (C. pyrenoidesa) to single and repeated exposures to silver nanoparticles (AgNPs). Repeated exposures to AgNPs promoted chlorophyll a and carotenoid production, and increased silver accumulation, thus enhancing the risk of AgNPs entering the food chain. Notably, the extracellular polymeric substances (EPS) content of the 1-AgNPs and 3-AgNPs groups were dramatically increased by 119.1% and 151.5%, respectively. We found that C. pyrenoidesa cells exposed to AgNPs had several significant alterations in metabolic process and cellular transcription. Most of the genes and metabolites are altered in a dose-dependent manner. Compared with the control group, single exposure had more differential genes and metabolites than repeated exposures. 562, 1341, 4014, 227, 483, and 2409 unigenes were differentially expressed by 1–0.5-AgNPs, 1–5-AgNPs, 1–10-AgNPs, 3–0.5-AgNPs, 3–5-AgNPs, and 3–10-AgNPs treatment groups compared with the control. Metabolomic analyses revealed that AgNPs altered the levels of sugars and amino acids, suggesting that AgNPs reprogrammed carbon/nitrogen metabolism. The changes of genes related to carbohydrate and amino acid metabolism, such as citrate synthase (CS), isocitrate dehydrogenase (IDH1), and malate dehydrogenase (MDH), further supported these results. These findings elucidated the mechanism of biological responses to repeated exposures to AgNPs, providing a new perspective on the risk assessment of nanomaterials.

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“…Another research field where the metabolomics of chlorophylls and carotenoids finds successful applications is the study of nanotoxicology, which aims to determine the toxicity of metals and micro-and nano-particles to environmental organisms and how the latter respond to the former. In this regard, it was studied how copper oxide nanoparticles, CuO microparticles, and copper ions perturb the metabolism of aquatics organisms [116] and even the effect of ZnO nanoparticles on the cultivation of terrestrial plants [117]. The most striking advance made in these studies is that the experimental design based in metabolic flux measurements might point out specific responses, which include chlorophyll breakdown and the tuning of carotenoids' metabolism.…”

Section: Application In Abiotic Factors Studiesmentioning

confidence: 99%

Metabolomics of Chlorophylls and Carotenoids: Analytical Methods and Metabolome-Based Studies

Roca

Pérez‐Gálvez

2021

Antioxidants

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Chlorophylls and carotenoids are two families of antioxidants present in daily ingested foods, whose recognition as added-value ingredients runs in parallel with the increasing number of demonstrated functional properties. Both groups include a complex and vast number of compounds, and extraction and analysis methods evolved recently to a modern protocol. New methodologies are more potent, precise, and accurate, but their application requires a better understanding of the technical and biological context. Therefore, the present review compiles the basic knowledge and recent advances of the metabolomics of chlorophylls and carotenoids, including the interrelation with the primary metabolism. The study includes material preparation and extraction protocols, the instrumental techniques for the acquisition of spectroscopic and spectrometric properties, the workflows and software tools for data pre-processing and analysis, and the application of mass spectrometry to pigment metabolomics. In addition, the review encompasses a critical description of studies where metabolomics analyses of chlorophylls and carotenoids were developed as an approach to analyzing the effects of biotic and abiotic stressors on living organisms.

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A global metabolomic insight into the oxidative stress and membrane damage of copper oxide nanoparticles and microparticles on microalga Chlorella vulgaris

Cited by 41 publications

References 51 publications

Antioxidant, Transcriptome and the Metabolome Response to Dietary Astaxanthin in Exopalaemon carinicauda

Antioxidant, Transcriptome and the Metabolome Response to Dietary Astaxanthin in Exopalaemon carinicauda

Transcriptomics and Metabolomics Revealed the Biological Response of Chlorella pyrenoidesa to Single and Repeated Exposures of AgNPs at Different Concentrations

Metabolomics of Chlorophylls and Carotenoids: Analytical Methods and Metabolome-Based Studies

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