A glucose biosensor based on glucose oxidase immobilized on three-dimensional porous carbon electrodes

Abstract

A novel glucose biosensor was developed by immobilizing glucose oxidase (GOD) on a three-dimensional (3D) porous kenaf stem-derived carbon (3D-KSC) which was firstly proposed as a novel supporting material to load biomolecules for electrochemical biosensing. Here, an integrated 3D-KSC electrode was prepared by using a whole piece of 3D-KSC to load the GOD molecules for glucose biosensing. The morphologies of integrated 3D-KSC and 3D-KSC/GOD electrodes were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The SEM results revealed a 3D honeycomb macroporous structure of the integrated 3D-KSC electrode. The TEM results showed some microporosities and defects in the 3D-KSC electrode. The electrochemical behaviors and electrocatalytic performance of the integrated 3D-KSC/GOD electrode were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The effects of pH and scan rates on the electrochemical response of the biosensor have been studied in detail. The glucose biosensor showed a wide linear range from 0.1 mM to 14.0 mM with a high sensitivity of 1.73 μA mM(-1) and a low detection limit of 50.75 μM. Furthermore, the glucose biosensor exhibited high selectivity, good repeatability and reproducibility, and good stability.

Direct electrochemistry and bioelectrocatalysis of glucose oxidase in CS/CNC film and its application in glucose biosensing and biofuel cells

“…This technique is a powerful tool for investigating interface properties of the modied electrode. 35 EIS was used to validate the interaction between GOx and CNCs. The EIS results of bare GCE (a), CS/GCE (b), CS/CNCs/GCE (c), and CS/GOx/CNCs/GCE (d) are shown in Fig.…”

A novel biosensor based on ball-flower-like Cu-hemin MOF grown on elastic carbon foam for trichlorfon detection

“…The sensitivity and the detection limit of these biosensors depend on the number of enzyme molecules, 14,15 and thus, immobilization of the enzyme on the surface of electrodes is a crucial step for the performance of the biosensors. In order to rmly immobilize the enzyme, numerous advanced materials such as carbon nanotubes, [16][17][18][19] gold nanoparticles, [20][21][22] and porous materials 23,24 have been introduced for the construction of enzyme-loaded matrixes. The integration of enzyme and porous materials greatly promoted the sensitivity, stability and detection limit of enzyme biosensors.…”

Al-Based porous coordination polymer derived nanoporous carbon for immobilization of glucose oxidase and its application in glucose/O2 biofuel cell and biosensor

“…[13][14][15][16] Among the various nanomaterials, carbon nanotubes (CNTs) and graphene (Gr) have been attracted enormous attentions for immobilization of GOx owing to their excellent physicochemical properties. 2,4,9,17 Hence, extensive efforts have been put forward to achieve synergistic integration of three-dimensional (3D) CNTs network or Gr with other nanomaterials as supporting materials for enzyme immobilization.…”