A Glycine soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa

Wu, Shengyang; Zhu, Pinghui; Jia, Bowei; Yang, Junkai; Shen, Yang; Cai, Xiaoxi; Zhu, Yanming; Sun, Mingzhe

doi:10.1186/s12870-018-1466-3

Cited by 23 publications

(16 citation statements)

References 44 publications

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“…In wheat, TabZIP15 promotes the combination of ABF/AREB and ABRE (ABA response element) cis-acting elements through the expression of ABF/AREB to induce downstream target gene expression responding to plant salt and drought stress [ 34 ]. Similar results were observed for the GsbZIP67 gene in Alfalfa ( Medicago sativa ), overexpression of GsbZIP67 promoted the growth of plant roots and shoots and changed the physiological indicators of transgenic plants under bicarbonate salt-alkali stress [ 32 ].…”

Section: Introductionsupporting

confidence: 59%

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Wang

Zhang

et al. 2022

BMC Plant Biol

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Background The basic leucine zipper (bZIP) transcription factor is one of the most abundant and conserved gene families in eukaryotes. In addition to participating in plant development and growth, bZIP transcription factors play crucial roles in various abiotic stress responses and anthocyanin accumulation. Up to now, analysis of bZIP gene family members in pomegranate (Punica granatum) has not been reported. Three published pomegranate genome sequences provide valuable resources for further gene function analysis. Results Using bioinformatics analysis, 65 PgbZIPs were identified and analyzed from the ‘Taishanhong’ pomegranate genome. We divided them into 13 groups (A, B, C, D, E, F, G, H, I, J, K, M, and S) according to the phylogenetic relationship with those of Arabidopsis, each containing a different number of genes. The regularity of exon/intron number and distribution was consistent with the classification of groups in the evolutionary tree. Transcriptome analysis of different tissues showed that members of the PgbZIP gene family were differentially expressed in different developmental stages and tissues of pomegranate. Among them, we selected PgbZIP16 and PgbZIP34 as candidate genes which affect anthocyanin accumulation. The full-length CDS region of PgbZIP16 and PgbZIP34 were cloned from pomegranate petals by homologous cloning technique, encoding 170 and 174 amino acids, which were 510 bp and 522 bp, respectively. Subcellular localization assays suggested that both PgbZIP16 and PgbZIP34 were nucleus-localized. Real-time quantitative PCR (qPCR) was used to explore the expression of PgbZIP16 and PgbZIP34 in the petals of three kinds of ornamental pomegranates at the full flowering stage. The results demonstrated that the expression of PgbZIP16 in red petals was 5.83 times of that in white petals, while PgbZIP34 was 3.9 times. The results of transient expression in tobacco showed that consistent trends were observed in anthocyanin concentration and expression levels of related genes, which both increased and then decreased. Both PgbZIP16 and PgbZIP34 could promote anthocyanin accumulation in tobacco leaves. We obtained transgenic strains overexpressing PgbZIP16, and the histochemical staining for GUS activity showed that overexpressed PgbZIP16 seedlings were expressed in the stem. Transgenic experiments indicated that overexpression of PgbZIP16 significantly upregulated UF3GT, ANS and DFR genes in Arabidopsis and enhanced anthocyanin accumulation. Conclusions The whole genome identification, gene structure, phylogeny, gene cloning, subcellular location and functional verification of the pomegranate bZIP gene family provide a theoretical foundation for the functional study of the PgbZIP gene family and candidate genes for anthocyanin biosynthesis.

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Section: Introductionsupporting

confidence: 59%

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Wang

Zhang

et al. 2022

BMC Plant Biol

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“…Recent studies have shown that bZIPs participate in bZIP transcriptional activation under bicarbonate-alkali stress and alter stress-related physiological indicators (such as reducing the accumulation of MDA and increasing both the activity of POD and the content of chlorophyll) and gene expression (excess GsbZIP67 expression in alfalfa) to improve salt-alkali tolerance (Wu et al, 2018). Members of the bZIP transcription factor family have been identified in a variety of higher plants.…”

Section: Induction Of Transcription Factor Expressionmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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As two coexisting abiotic stresses, salt stress and alkali stress have severely restricted the development of global agriculture. Clarifying the plant resistance mechanism and determining how to improve plant tolerance to salt stress and alkali stress have been popular research topics. At present, most related studies have focused mainly on salt stress, and salt-alkali mixed stress studies are relatively scarce. However, in nature, high concentrations of salt and high pH often occur simultaneously, and their synergistic effects can be more harmful to plant growth and development than the effects of either stress alone. Therefore, it is of great practical importance for the sustainable development of agriculture to study plant resistance mechanisms under saline-alkali mixed stress, screen new saline-alkali stress tolerance genes, and explore new plant salt-alkali tolerance strategies. Herein, we summarized how plants actively respond to saline-alkali stress through morphological adaptation, physiological adaptation and molecular regulation.

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“…Previous studies have reported that bZIP TFs were localized in nuclear [ 17 ], cytoplasmic [ 18 ], and endoplasmic reticulum [ 19 ]. Hence, to explore the subcellular localization of ChbZIP1 in plant cells, a ChbZIP1 sequence without the stop codon was constructed with the vector of pPZP211-GFP and transformed into Arabidopsis protoplasts.…”

Section: Resultsmentioning

confidence: 99%

“…Studies reported that bZIP TF could improve the survival of crop plants in alkaline conditions. A bZIP TF GsbZIP67, which was isolated from Glycine soja, overexpressed in alfalfa promoted plant growth under bicarbonate alkaline stress, as evidenced by longer roots and shoots [ 17 ]. DuanMu et al also found that the bZIP TFs family was significantly induced in the alkaline stress responses of wild soybean roots through transcriptome analysis [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Transcription Factor ChbZIP1 from Alkaliphilic Microalgae Chlorella sp. BLD Enhancing Alkaline Tolerance in Transgenic Arabidopsis thaliana

Show

Miao

2021

IJMS

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Saline-alkali soil has become an important environmental problem for crop productivity. One of the most effective approaches is to cultivate new stress-tolerant plants through genetic engineering. Through RNA-seq analysis and RT-PCR validation, a novel bZIP transcription factor ChbZIP1, which is significantly upregulated at alkali conditions, was obtained from alkaliphilic microalgae Chlorella sp. BLD. Overexpression of ChbZIP1 in Saccharomyces cerevisiae and Arabidopsis increased their alkali resistance, indicating ChbZIP1 may play important roles in alkali stress response. Through subcellular localization and transcriptional activation activity analyses, we found that ChbZIP1 is a nuclear-localized bZIP TF with transactivation activity to bind with the motif of G-box 2 (TGACGT). Functional analysis found that genes such as GPX1, DOX1, CAT2, and EMB, which contained G-box 2 and were associated with oxidative stress, were significantly upregulated in Arabidopsis with ChbZIP1 overexpression. The antioxidant ability was also enhanced in transgenic Arabidopsis. These results indicate that ChbZIP1 might mediate plant adaptation to alkali stress through the active oxygen detoxification pathway. Thus, ChbZIP1 may contribute to genetically improving plants’ tolerance to alkali stress.

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A Glycine soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa

Cited by 23 publications

References 44 publications

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Response Mechanisms of Plants Under Saline-Alkali Stress

Transcription Factor ChbZIP1 from Alkaliphilic Microalgae Chlorella sp. BLD Enhancing Alkaline Tolerance in Transgenic Arabidopsis thaliana

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