A glycolytic product is obligatory for initiation of the sperm acrosome reaction and whiplash motility required for fertilization in the mouse

Fraser, Lynn R.; Quinn, P. J.

doi:10.1530/jrf.0.0610025

Cited by 201 publications

(164 citation statements)

References 16 publications

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“…It would therefore appear that the introduction of albumin triggers the acrosome reaction in those cells that otherwise would have lost the acrosome during the incubation phase. A similar response was noted when glucose was introduced to suspensions capacitated in glucose-free medium (Fraser & Quinn, 1981). Whether the fertilizing spermatozoa are drawn from this pool, however, or from the acrosome-intact cells with the potential to undergo the acrosome reaction is unclear.…”

Section: Discussionmentioning

confidence: 66%

“…After preincubation for 30 or 120 min, aliquants of sperm suspensions were diluted 10-fold, incubated for 5 min and then subjected to filtration on short columns prepared in Pasteur pipettes (Fraser & Quinn, 1981 ) to select motile, potentially fertilizing spermatozoa for assessment. Preliminary studies indicated that a macromolecular component (PVA) in the medium and a column made of Sephadex G-25 (Fraser & Quinn, 1981), rather than glass beads (Fraser, 1983b), prevented excessive sticking of cells during filtration which might distort the results. This problem does not arise when BSA is included in the medium.…”

Section: Methodsmentioning

confidence: 99%

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Albumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro

Fraser¹

1985

Reproduction

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Summary. Albumin was required specifically for penetration of the zona pellucida (<10% of eggs fertilized in the absence of albumin), but was not required for capacitation. A similar rate of capacitation was observed in the presence of albumin at concentrations ranging from 30 to 1 mg/ml, while a slightly slower rate was observed in the presence of 0\m=.\25and 0\m=.\1 mg albumin/ml. In the absence of albumin, capacitation occurred at a rate which lagged behind that of the albumin-incubated counterparts by about 30 min; once capacitated, the addition of albumin promoted rapid sperm penetration. In albumin-free media ( \m=+-\the macromolecule PVA), sperm motility was frequently reduced, with fewer cells exhibiting hyperactivated motility, but improvements were observed after introduction of albumin. Acrosome loss was significantly lower in the absence of albumin, but within 5 min of its addition at concentrations ranging from 30 to 0\m=.\1 mg/ml to capacitated sperm suspensions, acrosome loss was stimulated and reached levels similar to those seen in control samples. Therefore, albumin can trigger the acrosome reaction in capacitated spermatozoa. It appears to act by assisting in the removal of a surface-associated inhibitory component, the presence of which stabilizes the sperm membranes and inhibits the acrosome reaction.

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Section: Discussionmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Albumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro

Fraser¹

1985

Reproduction

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“…In addition, hyperactivated motility may help the sperm to penetrate the egg vestments (Suarez et al 1991;Stauss et al 1995;Ho and Suarez 2001). In rodents, and probably in other species, hyperactivated sperm motility is also correlated with the ability of a sperm to fertilise an oocyte in vitro (Fraser and Quinn 1981;Boatman and Robbins 1991). Thus, the bulk of evidence suggests that a normal sperm must be able to acquire both activated motility in the caudal female reproductive tract and uterus and hyperactivated motility in the oviduct for it to have a reasonable chance of fertilising the egg.…”

Section: Two Types Of Physiological Mammalian Sperm Motilitymentioning

confidence: 99%

“…It has also been shown that mammalian sperm produce lactate from glucose under aerobic conditions (Storey and Kayne 1975). Adenosine triphosphate production through glycolysis is required for hyperactivated sperm motility (Hoshi et al 1991;Urner and Sakkas 1996) and inhibition of oxidative phosphorylation does not block fertilisation (Fraser and Quinn 1981).…”

Section: Fuel Support and More: The Fibrous Sheathmentioning

confidence: 99%

Moving to the beat: a review of mammalian sperm motility regulation

Turner¹

2006

Reprod. Fertil. Dev.

248

184

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Abstract. Because it is generally accepted that a high percentage of poorly motile or immotile sperm will adversely affect male fertility, analysis of sperm motility is a central part of the evaluation of male fertility. In spite of its importance to fertility, poor sperm motility remains only a description of a pathology whose underlying cause is typically poorly understood. The present review is designed to bring the clinician up to date with the most current understanding of the mechanisms that regulate sperm motility and to raise questions about how aberrations in these mechanisms could be the underlying causes of this pathology.

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“…Spermatozoa were incubated under conditions which support capacitation (Fraser, 1983) and modified conditions which compromise expression of fertilizing ability (Fraser & Quinn, 1981;Fraser, 1982Fraser, , 1983Fraser, , 1987. The standard incubation medium supports capacitation, which is generally complete within 120 min.…”

Section: Introductionmentioning

confidence: 99%

Enzymes of adenosine metabolism in mouse sperm suspensions

Monks

Fraser²

1988

Reproduction

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Summary. The enzymes of adenosine metabolism were investigated in suspensions of epididymal mouse spermatozoa incubated under conditions which support capacitation in vitro. High levels of adenosine deaminase activity were found in sperm suspensions, but the enzyme was located in the surrounding medium and was not intrinsic to spermatozoa. 5\m='\-Nucleotidase was also present in the surrounding medium while in sperm cells it existed as an ecto-enzyme. Adenosine was not metabolized by washed spermatozoa under conditions used for the assay of adenosine deaminase or adenosine kinase, but it was metabolized rapidly by unwashed sperm suspensions. Incubation of sperm suspensions in conditions which modulate fertilizing ability resulted in small alterations in intrinsic 5\m='\-nucleotidase activity of spermatozoa. In contrast, the activity of adenosine deaminase was not consistently modulated by such maniupulations.Adenosine deaminase and 5\m='\-nucleotidase exhibited similar kinetic parameters to enzymes from other sources and their activities were inhibited by coformycin and \ g = a \ , \ g = b \ \ x = r e q -\ methylene adenosine 5\m='\-diphosphate, respectively. These studies highlight the low adenosine-metabolizing ability of spermatozoa coupled with the extensive metabolism in the medium which surrounds them. Extracellular adenosine metabolism can therefore occur and may modulate capacitation in vitro.

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A glycolytic product is obligatory for initiation of the sperm acrosome reaction and whiplash motility required for fertilization in the mouse

Cited by 201 publications

References 16 publications

Albumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro

Albumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro

Moving to the beat: a review of mammalian sperm motility regulation

Enzymes of adenosine metabolism in mouse sperm suspensions

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