A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls

Yang, Gangyi; Zhuang, Huisheng; Chen, Hanyu; Ping, Xianyin; Bu, Dan

doi:10.1016/j.snb.2015.02.128

Cited by 35 publications

(23 citation statements)

References 23 publications

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“…3. Based on the high specificity and amplifying efficiency of real-time PCR, the optimal combination of reagents corresponded to the minimum Ct value28. Therefore, the optimal concentration of hapten was 0.80 mg L −1 , and the optimal concentration of antibody was 1.51 mg L −1 .…”

Section: Resultsmentioning

confidence: 99%

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A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

Jin

Chen

et al. 2016

Sci Rep

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A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL−1 to 10 ng mL−1, and the limit of detection (LOD) was 0.02 ng mL−1, which was 10–20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.

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Section: Resultsmentioning

confidence: 99%

“…Gold nanoparticles (AuNPs) probes were prepared following a previously reported procedure with a few modifications2833. Figure 1A illustrates the schematic diagram of the fabrication of the AuNPs probe.…”

Section: Methodsmentioning

confidence: 99%

A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

Jin

Chen

et al. 2016

Sci Rep

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“…These values are comparable to similar reported β-diketonate complexes. 27,28 This suggests that the presence of a molecule of methanol coordinated to the lanthanoid cations in complexes 3 and 7 is not dramatically quenching the NIR emission which might be explained by the presence of intramolecular hydrogen bonds with short O…O distances involving the methanol OH groups in both structures (3: O10…O22, 2.70(1); O20…O12, 2.74(1). 7: O1…O22, 2.576(3); O2…O51, 2.578(3) Å).…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 98%

Lanthanoid Complexes Supported by Retro-Claisen Condensation Products of β-Triketonates

Galán¹,

Sobolev²,

Zysman‐Colman³

et al. 2018

Preprint

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β-Triketonates have been recently used as chelating ligands for lanthanoid ions, presenting unique structures varying from polynuclear assemblies to polymers. In an effort to overcome low solubility of the complexes of tribenzoylmethane, four β-triketones with higher lipophilicity were synthesised. Complexation reactions were performed for each of these molecules using different alkaline bases in alcoholic media. X-ray diffraction studies suggested that the ligands were undergoing decomposition under the reaction conditions. This is proposed to be caused by in situretro-Claisen condensation reactions, consistent with two examples that have been reported previously. The lability of the lanthanoid cations in the presence of a varying set of potential ligands gave rise to structures where one, two, or three of the molecules involved in the retro-Claisen condensationreaction were linked to the lanthanoid centres. These results, along with measurements of ligand decomposition in the presence of base alone, suggest that using solvents of lower polarity will mimimise the impact of the retro-Claisen condensation in these complexes.

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“…Polychlorinated biphenyls (PCBs) are ubiquitous pollutants that are widely spread in the environment [2] and extensively applied in different branches of industry, owing to their excellent chemical properties, physical properties [3], lack of combustibility, thermal stability, and dielectric properties. In addition, PCBs are broadly employed in various industries as insulating fluids and coolants in electrical tools in power plants and huge buildings [4][5][6]. Since the 1970s, the production and commercial use of PCBs has been prohibited in some countries because of their bioaccumulation, environmental persistence, and strong toxicity [1].…”

Section: Introductionmentioning

confidence: 99%

3A-Amino-3A-Deoxy-(2AS, 3AS)-β-Cyclodextrin Hydrate/Tin Disulfide Modified Screen-Printed Carbon Electrode for the Electrochemical Detection of Polychlorinated Biphenyls

et al. 2020

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Polychlorinated biphenyls (PCBs) are persistent organic pollutants that are widely distributed in the environment. It is noteworthy that the PCBs are endocrine-disrupting substances, and their toxicity induces cancer and damage to the mammalian reproductive system, immune system, stomach, skin, liver, etc. This work aimed to synthesize 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate/tin disulfide composite material and to study its material properties, electrochemical properties, and application to PCB detection. The nanostructured tin disulfide (SnS 2) synthesized by hydrothermal technique and 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate were sequentially modified onto the disposable screen-printed carbon electrode (SPCE) via titration using a micropipette. The 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate (β-CD) improved the selectivity of the modified electrode. The fabricated β-CD/SnS 2 /SPCE was employed to determine the presence of PCBs by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The detection range was 0.625-80 μM, with a limit detection of approximately 5 μM. The electrodes were as stable as 88% after 7 days' storage. The results showed that the β-CD successfully encapsulated PCBs to achieve an electrochemical sensor that reduced the time and increased the convenience of PCBs detection.

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A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls

Cited by 35 publications

References 23 publications

A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

Lanthanoid Complexes Supported by Retro-Claisen Condensation Products of β-Triketonates

3A-Amino-3A-Deoxy-(2AS, 3AS)-β-Cyclodextrin Hydrate/Tin Disulfide Modified Screen-Printed Carbon Electrode for the Electrochemical Detection of Polychlorinated Biphenyls

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