A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

Abstract

A simple and ultra-sensitive fluorescent sensor was developed to detect the enzymatic activity of Exo III with a limit of detection of 0.001 U mL−1.

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

“…Because of the advantages of fast response, noncontamination, and high specificity, FRET aptasensor has been extensively exploited in lysozyme detection studies [9][10][11][12][13][14]. To achieve sensitivity improvement, FRET sensors based on various materials, structures, and probes have been reported [15][16][17][18][19]. Among the materials employed in the FRET sensors, graphene oxide (GO) is most commonly used because of its tunable fluorescent emission efficiency [15][16][17].…”

“…To achieve sensitivity improvement, FRET sensors based on various materials, structures, and probes have been reported [15][16][17][18][19]. Among the materials employed in the FRET sensors, graphene oxide (GO) is most commonly used because of its tunable fluorescent emission efficiency [15][16][17]. e FRET sensor structures for amplifying fluorescence signals can be divided into two types.…”

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

“…Graphene and graphene oxide (GO) represent emerging nanomaterials in biosensing. They combine exceptional optical, electrochemical, and electronic properties with an excellent fluorescence quenching activity [56][57][58]. Various MB-based biosensing approaches have been investigated by exploiting the spontaneous adsorption of DNA on GO via π-π stacking and hydrogen bonding [59].…”

Tango of dual nanoparticles: Interplays between exosomes and nanomedicine

“…Two‐dimensional NPs have a structure of single atomic layer and incline to form π‐π electron stacking interactions with biological and chemical molecules like DNA, proteins and fluorescent molecules, thus possessing a high distance‐dependent fluorescence quenching capability 99,100 . In recent years, different types of 2D NPs have raised great interests for their potential applications as a nanoquencher in FRET‐based biosensor platform.…”