A graphene-sandwiched DNA nano-system: regulation of intercalated doxorubicin for cellular localization

Nandi, Semonti; Kale, Narendra; Patil, Ashwini; Banerjee, Soumik; Patil, Yuvraj; Khandare, Jayant

doi:10.1039/d0na00575d

Cited by 3 publications

(1 citation statement)

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“…Although DOX shows nuclear localization, but it depends on the time of incubation. For confocal study, we incubated the free DOX and hydrogel loaded DOX for 2 h only in which nuclear localization is very less and nanotized DOX shows more cytoplasmic localization compared to free DOX. , Further, the goal of bio-imaging was to check the bioavailability of the DOX when loaded in the hydrogel. Overall, the gDNA–DNA Dots hydrogel whether pristine or loaded with drugs showed smooth internalization into cells.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Sustained Drug Delivery, Tracking, and On-Demand Photoactivation of DNA–Hydrogel Formulated from a Biomass-Derived DNA Nanoparticle

Shankar

Nayak

Singh

et al. 2023

ACS Appl. Bio Mater.

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Conversion of biomass into nanoparticles for meaningful biomedical applications is a formidable proposition with excellent prospects but fewer patrons. A lack of general methodology for upscaled production and limited versatility of those nanoparticles are the main drawbacks. Herein, we report the creation of a DNA nanoparticle (DNA Dots) from onion genomic DNA (gDNA), a plant biomass source, through controlled hydrothermal pyrolysis in water without any chemicals. The DNA Dots are further formulated into a stimuli-responsive hydrogel through hybridization-mediated self-assembly with untransformed precursor gDNA. The versatility of the DNA Dots is recognized through its crosslinking ability with gDNA through its dangling DNA strands on the surface resulting from incomplete carbonization during annealing without the need for any external organic, inorganic, or polymeric crosslinkers. The gDNA–DNA Dots hybrid hydrogel is shown to be an excellent drug delivery vehicle for sustained release trackable through the inherent fluorescence of the DNA Dots. Interestingly, the DNA Dots are photoexcited with normal visible light to generate on-demand reactive oxygen species, making them exciting candidates for combination therapeutics. Most importantly, the ease with which the hydrogel is internalized in fibroblast cells with minimal cytotoxicity should encourage the nanotization of biomass as a tool for interesting sustainable biomedical applications.

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Section: Resultsmentioning

confidence: 99%