A green approach to obtain stable and hydrophilic cellulose-based electrospun nanofibrous substrates for sustained release of therapeutic molecules

Kurečič, Manja; Mohan, Tamilselvan; Virant, Natalija; Maver, Uroš; Stergar, Janja; Gradišnik, Lidija; Kleinschek, Karin Stana; Hribernik, Silvo

doi:10.1039/c9ra03399h

Cited by 21 publications

(11 citation statements)

References 56 publications

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“…The observation demonstrates the significant effect of small-molecule additives for controlling the self-assembly of cellulose oligomers for the creation of hierarchically structured materials in a simple manner. This study will open a new scientific or technological world of nanostructured cellulose oligomers, which is different from that of naturally derived cellulosic materials [47–50].…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic oligomerization-induced self-assembly of crystalline cellulose oligomers into nanoribbon networks assisted by organic solvents

Hata¹,

Fukaya²,

Sawada³

et al. 2019

Beilstein J. Nanotechnol.

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Crystalline poly- and oligosaccharides such as cellulose can form extremely robust assemblies, whereas the construction of self-assembled materials from such molecules is generally difficult due to their complicated chemical synthesis and low solubility in solvents. Enzyme-catalyzed oligomerization-induced self-assembly has been shown to be promising for creating nanoarchitectured crystalline oligosaccharide materials. However, the controlled self-assembly into organized hierarchical structures based on a simple method is still challenging. Herein, we demonstrate that the use of organic solvents as small-molecule additives allows for control of the oligomerization-induced self-assembly of cellulose oligomers into hierarchical nanoribbon network structures. In this study, we dealt with the cellodextrin phosphorylase-catalyzed oligomerization of phosphorylated glucose monomers from ᴅ-glucose primers, which produce precipitates of nanosheet-shaped crystals in aqueous solution. The addition of appropriate organic solvents to the oligomerization system was found to result in well-grown nanoribbon networks. The organic solvents appeared to prevent irregular aggregation and subsequent precipitation of the nanosheets via solvation for further growth into the well-grown higher-order structures. This finding indicates that small-molecule additives provide control over the self-assembly of crystalline oligosaccharides for the creation of hierarchically structured materials with high robustness in a simple manner.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic oligomerization-induced self-assembly of crystalline cellulose oligomers into nanoribbon networks assisted by organic solvents

Hata¹,

Fukaya²,

Sawada³

et al. 2019

Beilstein J. Nanotechnol.

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“…The influence of the digestive inflammatory environment (DIE) was most remarkable in terms of the kinetic protein release profile. The sustained release of anti-inflammatory cytokines in the inflammatory environment is very crucial to modulate the inflammatory response [ 64 , 65 ]. Thus, the immunomodulatory scaffolds should possess a controlled-release profile of encapsulated biologics in the inflammatory environment.…”

Section: Discussionmentioning

confidence: 99%

Impact of Digestive Inflammatory Environment and Genipin Crosslinking on Immunomodulatory Capacity of Injectable Musculoskeletal Tissue Scaffold

Shortridge

Fakhrabadi

Wuescher

et al. 2021

IJMS

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The paracrine and autocrine processes of the host response play an integral role in the success of scaffold-based tissue regeneration. Recently, the immunomodulatory scaffolds have received huge attention for modulating inflammation around the host tissue through releasing anti-inflammatory cytokine. However, controlling the inflammation and providing a sustained release of anti-inflammatory cytokine from the scaffold in the digestive inflammatory environment are predicated upon a comprehensive understanding of three fundamental questions. (1) How does the release rate of cytokine from the scaffold change in the digestive inflammatory environment? (2) Can we prevent the premature scaffold degradation and burst release of the loaded cytokine in the digestive inflammatory environment? (3) How does the scaffold degradation prevention technique affect the immunomodulatory capacity of the scaffold? This study investigated the impacts of the digestive inflammatory environment on scaffold degradation and how pre-mature degradation can be prevented using genipin crosslinking and how genipin crosslinking affects the interleukin-4 (IL-4) release from the scaffold and differentiation of naïve macrophages (M0). Our results demonstrated that the digestive inflammatory environment (DIE) attenuates protein retention within the scaffold. Over 14 days, the encapsulated protein released 46% more in DIE than in phosphate buffer saline (PBS), which was improved through genipin crosslinking. We have identified the 0.5 (w/v) genipin concentration as an optimal concentration for improved IL-4 released from the scaffold, cell viability, mechanical strength, and scaffold porosity, and immunomodulation studies. The IL-4 released from the injectable scaffold could differentiate naïve macrophages to an anti-inflammatory (M2) lineage; however, upon genipin crosslinking, the immunomodulatory capacity of the scaffold diminished significantly, and pro-inflammatory markers were expressed dominantly.

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“…The swelling kinetics of the Ink x / y / N scaffolds in biofluid (ADMEM +5% FBS +100 IU mL −1 penicillin and 0.1 mg mL −1 streptomycin) were investigated using a gravimetric method ( Kurečič et al., 2019 ; Mohan et al., 2020 ). The dried cylinder-shaped scaffolds ( r = 7 mm, h = 5 mm) were weighed (initial weight, W 0 ), immersed in 5 mL biofluid (pH 7.4) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Organic acid cross-linked 3D printed cellulose nanocomposite bioscaffolds with controlled porosity, mechanical strength, and biocompatibility

et al. 2022

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A green approach to obtain stable and hydrophilic cellulose-based electrospun nanofibrous substrates for sustained release of therapeutic molecules

Cited by 21 publications

References 56 publications

Biocatalytic oligomerization-induced self-assembly of crystalline cellulose oligomers into nanoribbon networks assisted by organic solvents

Biocatalytic oligomerization-induced self-assembly of crystalline cellulose oligomers into nanoribbon networks assisted by organic solvents

Impact of Digestive Inflammatory Environment and Genipin Crosslinking on Immunomodulatory Capacity of Injectable Musculoskeletal Tissue Scaffold

Organic acid cross-linked 3D printed cellulose nanocomposite bioscaffolds with controlled porosity, mechanical strength, and biocompatibility

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