A Green Product Using Selective Compound for Susceptible Assessment of Copper in Blood Serum

Abstract

A highly reliable approach for assessment of copper(II) in blood serum samples using an organic molecule 4-(3-hydroxyphenylazo)resorcinol, 4-HAR was developed. The paper presents the synthesis of 4-HAR, which serves as probe for definite species. 4-HAR was characterized with the FT-IR, UV-visible, 1H-NMR, 13C-NMR and mass analysis, thermal TG and DSC techniques. After examining its interactions with more than twenty-five metal ions, it was found that 4-HAR showed reaction only with copper(II), giving a distinct green color at λmax 603 nm. Experimental conditions for the reaction of 4-HAR with copper(II) were optimized. A calibration curve was created and the exhibition range of concentration obeyed Beer's Law from 0.30 to 16 μg.mL-1 with correlation coefficient of r = 0.9986, and value of the molar absorption coefficient, ε, being 3314 L.mol- 1.cm-1. The analytical method was used to determine copper(II) levels in several blood serum samples. The amounts of copper(II) found in the sera samples were compared with flame atomic absorption spectrometry technique, and the results were found to be highly reliable. Based on validation, the new approach can be utilized for assurance quality purposes with a high degree of confidence.

Spectrophotometric Determination and Biological Activity Study of Zn(II) using a Newly Synthesized Azo Reagent Derivative from 4,5-bis(4-methoxyphenyl) Imidazole

“…Azo compounds can be considered an important group of organic compounds that contain one or more azo chromophores (-N=N-bridge), which gives different characteristic colours to this well-known type of chemicals [1][2][3][4]. Heterocyclic azo compounds are very active toward the transition metal ions because they contain active donor groups and possess atoms such as nitrogen, oxygen, and sulfur, enabling them to form chelating coordination complexes, which are important in the biological field [5].…”

“…Azo compounds are involved in some biological reactions, such as RNA and DNA inhibition, protein synthesis, and nitrogen fixation [8], they have many advantages, such as ease of preparation, abundant product, high selectivity and sensitivity, and desirable stability. These advantages make this class of chemicals highly used in different applications [9,10], and utilized successfully as anticancer-treating chemicals [11,12]. Zinc metal is an important component of the human body and a required cofactor for some intracellular proteins [13,14].…”

Synthesis and characterization of a new azo quinoline ligand and its metal complexes with spectrophotometric determination and biological efficacy study of its Hg(II) complex

“…Azo compounds are a significant group of organic chemical compounds, and these compounds have one or more azo chromophores as (-N=N-), azo chromophore gives characteristic colors to these compounds [1]. Azo compounds can be employed as analytical reagents for the microquantity determination of different elements in multiple specimens [2][3][4]. Azo dyes are considered to be in general nonbiodegradable molecules under aerobic conditions and can be reduced to hazardous intermediate compounds under anaerobic conditions [5].…”