“…Furthermore, the latter nine isolates formed three subgroups, as follows: (i) three of these strains were lysed by all five hages isolated for strains MKD8, MKD10, MKD19, MKD35 , and MKD38; (ii) two strains (TMS14 and MKD38) were not lysed by $10 even if high phage titers were used, and one strain (MKD35T) was lysed by this phage only if a high phage titer was used; and (iii) three strains (MKD10, MKD19, and MKD57) were sensitive to phages $35 and $38 only if high phage titers were used. These results also revealed the following problems that arise in phage typing studies: (i) more or less strong restriction barriers by some strains within a genus, as described previously for Succharopolysporu hirsutu and Sacchuropolyspora elythrueu (55,56) and some strains of Streptornyces coeZicoZor Miiller (53) and also found for MKD19 and CHB77T; (ii) occurrence of lysogeny in some strains and repression of homoimmune phages (55); and (iii) temperate phages that have the ability to lysogenize their hosts (22,54,56) (this property was also observed with phage $10, which lysogenized four of the isolates, MKD19, MKD35T, MKD57, and TMD78).…”