Thermocrispum gen. nov., a New Genus of the Order Actinomycetales, and Description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.

Korn-Wendisch, Felicitas; Rainey, Frederick A.; Kroppenstedt, R. M.; Kempf, Alexander; Majazza, A.; Kutzner, H. J.; Stackebrandt, Erko

doi:10.1099/00207713-45-1-67

Cited by 50 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Later on, when temperatures started to decrease, new bands appeared that represented other kinds of Actinobacteria, for example, the thermo‐tolerant genera Thermocrispum and Actinomadura . These organisms have previously been found in compost or compost‐amended soil respectively (Kornwendisch et al. 1995; Ibekwe et al.…”

Section: Discussionmentioning

confidence: 89%

Development of compost maturity and Actinobacteria populations during full-scale composting of organic household waste

Steger¹,

Sjögren²,

Jarvis³

et al. 2007

J Appl Microbiol

View full text Add to dashboard Cite

Aims: This study investigates changes in microbiological and physicochemical parameters during large‐scale, thermophilic composting of a single batch of municipal organic waste. The inter‐relationships between the microbial biomass and community structure as well as several physicochemical parameters and estimates of maturation were evaluated. Methods and Results: Analyses of signature fatty acids with the phospholipid fatty acid and ester‐linked methods showed that the total microbial biomass was highest during the early thermophilic phase. The contribution of signature 10Me fatty acids from Actinobacteria indicated a relatively constant proportion around 10% of the microbial community. However, analyses of the Actinobacteria species composition with a PCR‐denaturing gradient gel electrophoresis approach targeting 16S rRNA genes demonstrated clear shifts in the community structure. Conclusions: This study demonstrates that compost quality, particularly maturity, is linked to the composition of the microbial community structure, but further studies in other full‐scale systems are needed to validate the generality of these findings. Significance and Impact of the Study: The combination of signature lipid and nucleic acid‐based analyses greatly expands the specificity and the scope for assessing the microbial community composition in composts. The results presented in this study give new information on how the development of the compost microbial community is connected to curing and maturation in the later stages of composting, and emphasizes the role of Actinobacteria in this respect.

show abstract

Section: Discussionmentioning

confidence: 89%

Development of compost maturity and Actinobacteria populations during full-scale composting of organic household waste

Steger¹,

Sjögren²,

Jarvis³

et al. 2007

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…The genus Saccharopolyspora forms an extensive substrate mycelium that fragments and an aerial mycelium that does not fragment (Lu et al, 2001). The genus Thermocrispum forms a white aerial mycelium and yellow to light-brown vegetative mycelium (Korn-Wendisch et al, 1995).…”

Section: Digested Withmentioning

confidence: 99%

Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns

Cook

2003

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

138

104

View full text Add to dashboard Cite

A rapid method for identifying filamentous actinomycete genera was developed based on 16S rRNA gene restriction fragment patterns. The patterns were generated by using specific restriction endonucleases to perform in silico digestions on the 16S rRNA gene sequences of all validly published filamentous actinomycete species. The method was applied to identifying actinomycete isolates from soil. Amplified 16S rDNA of soil actinomycetes was restricted with selected endonucleases and electrophoresed on agarose gels. The restriction fragment patterns of the unknown isolates were easily compared to the established patterns. Significantly, the genus Streptomyces could be differentiated from all other actinomycete genera by using only four restriction endonucleases, Sau3AI, AsnI, KpnI and SphI. This could be achieved in a time period of as little as a week, following PCR-template DNA isolation by a simple method. The identification method allowed unknown, non-Streptomyces soil isolates to be identified to a genus or small subgroup of genera. The genera in these subgroups could, in some cases, be distinguished by virtue of colony-morphology differences.

show abstract

“…This thermophilic organism was isolated from municipal waste compost. [11] TmCHMO clusters with known CHMOs sequences (Figures S1, S2 in the Supporting Information), the closest homologue being from Brachymonas petroleovorans (66 % sequence identity). We identified three genes upstream of the TmCHMO gene that may participate in cyclohexanol degradation (Table S2).…”

mentioning

confidence: 99%

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

et al. 2016

View full text Add to dashboard Cite

Cyclohexanone monooxygenase (CHMO) is ap romising biocatalyst for industrial reactions owing to its broad substrate spectrum and excellent regio-, chemo-, and enantioselectivity.However,the lowstability of many BaeyerVilliger monooxygenases is an obstacle for their exploitation in industry.C haracterization and crystal structure determination of ar obust CHMO from Thermocrispum municipale is reported. The enzyme efficiently converts avariety of aliphatic, aromatic,a nd cyclic ketones,a sw ell as prochiral sulfides.A compact substrate-binding cavity explains its preference for small rather than bulky substrates.S mall-scale conversions with either purified enzyme or whole cells demonstrated the remarkable properties of this newly discovered CHMO.T he exceptional solvent tolerance and thermostability make the enzyme very attractive for biotechnology.

show abstract

Thermocrispum gen. nov., a New Genus of the Order Actinomycetales, and Description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.

Cited by 50 publications

References 24 publications

Development of compost maturity and Actinobacteria populations during full-scale composting of organic household waste

Development of compost maturity and Actinobacteria populations during full-scale composting of organic household waste

Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

Contact Info

Product

Resources

About