A growth factor- and hormone-stimulated NADH oxidase from rat liver plasma membrane

Brightman, Andrew O.; Wang, Juan; Miu, Raymond Kin-man; Sun, I. L.; Barr, Rita; Crane, F.L.; Morré, D. James

doi:10.1016/0005-2736(92)90168-l

Cited by 136 publications

(66 citation statements)

References 29 publications

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“…Although Zn (II) itself is reported to be an inducer of oxidative stress by promoting mitochondrial and extra-mitochondrial production of ROS 15 , the generation of ROS at a nontoxic level upon Zn (II) exposure/release has not been described. Nevertheless, O2 •− -producing NAD(P)H oxidases are activated in response to growth factors and oncogenes, and enhanced cell proliferation was observed upon ROS-inducing, low-dose photodynamic treatment [38][39][40][41][42] . Therefore, simultaneous increases in cell proliferation and ROS levels are not conflicting.…”

Section: Discussionmentioning

confidence: 99%

Effects of non-toxic zinc exposure on human epidermal keratinocytes

et al. 2015

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Zinc is an essential microelement; its importance to skin is shown by severe skin symptoms in hereditary or acquired zinc deficiency, by the improvement of several skin conditions using systemic or topical zinc preparations and by the induced intracellular zinc release upon UVB exposure, which is the main harmful environmental factor to skin. Understanding the molecular background of the role of zinc in skin may help to gain insight into the pathology of skin disorders and to provide evidence for the therapeutic usefulness of zinc supplementation.Herein, we studied the effect of zinc chloride (ZnCl 2 ) exposure on the function of HaCaT keratinocytes, and we found that a non-toxic elevation in the concentration of extracellular zinc (100 µM) facilitated cell proliferation and induced significant alterations in the mRNA expression of NOTCH1, IL8, and cyclooxygenase-2. In addition, we detected increased heme oxygenase-1 (HMOX1) expression and non-toxic generation of superoxide in the first 4 h.Regarding the effect on UVB-induced toxicity, although the level of cyclobutane pyrimidine dimers in keratinocytes pre-treated with zinc for 24 h was reduced 3 h after UVB irradiation, we found significantly enhanced superoxide generation 10 h after UVB exposure in the zinc pre-exposed cells. The overall survival was unaffected; however, there was a decrease in the percentage of early apoptotic cells and an increase in the percentage of late apoptotic plus necrotic cells.Our results suggest that exposure of human keratinocytes to non-toxic concentrations of ZnCl 2 impacts gene expression, cell proliferation and the response to environmental stress in skin. It would be important to further examine the role of zinc in skin and to further clarify if this issue can affect our thinking about the pathogenesis of skin diseases.

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Section: Discussionmentioning

confidence: 99%

Effects of non-toxic zinc exposure on human epidermal keratinocytes

et al. 2015

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“…13 This could be mediated by ferric ion acting as an electron acceptor for a trans-plasma membrane redox system. 41 Plasma membrane NADH oxidase is a key enzyme of this system and plays an important role for cell proliferation, and its inhibition induces apoptosis. 42 A high constitutive activity of NADH oxidase, in plasma membranes of nodular cells, has been attributed to an excess of diferric Tf/TF-R complexes on cell surface in proximity of this enzymatic system.…”

Section: Discussionmentioning

confidence: 99%

Transferrin and transferrin receptor gene expression and iron uptake in hepatocellular carcinoma in the rat

et al. 1998

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“…A second instrument with the vehicle alone added at 60 min served as the control. An extinction coefficient of 6.21 mM −1 ·cm −1 was used to calculate the specific activity [11].…”

Section: Nad(p)h Oxidase Assaymentioning

confidence: 99%

Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in <i>Saccharomyces cerevisiae</i>

Dick¹,

Ryuzoji²,

Morré³

et al. 2013

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A growth factor- and hormone-stimulated NADH oxidase from rat liver plasma membrane

Cited by 136 publications

References 29 publications

Effects of non-toxic zinc exposure on human epidermal keratinocytes

Effects of non-toxic zinc exposure on human epidermal keratinocytes

Transferrin and transferrin receptor gene expression and iron uptake in hepatocellular carcinoma in the rat

Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in <i>Saccharomyces cerevisiae</i>

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A growth factor- and hormone-stimulated NADH oxidase from rat liver plasma membrane

Cited by 136 publications

References 29 publications

Effects of non-toxic zinc exposure on human epidermal keratinocytes

Effects of non-toxic zinc exposure on human epidermal keratinocytes

Transferrin and transferrin receptor gene expression and iron uptake in hepatocellular carcinoma in the rat

Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt;

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Identification of the constitutive ultradian oscillator of the circadian clock (ENOX1) in <i>Saccharomyces cerevisiae</i>