A GT-rich Sequence Binding the Transcription Factor Sp1 Is Crucial for High Expression of the Human Type VII Collagen Gene (COL7A1) in Fibroblasts and Keratinocytes

Vindevoghel, Laurence; Chung, Kee Yang; Davis, Angelique M.; Kouba, David J.; Kivirikko, Sirpa; Alder, Hansjüerg; Uitto, Jouni; Mauviel, Alain

doi:10.1074/jbc.272.15.10196

Cited by 39 publications

(55 citation statements)

References 43 publications

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“…The human COL7A1 promoter construct, 7524COL7A1/ CAT has been described previously (Vindevoghel et al, 1997). Mutations within Smad and AP-1 sites were performed by PCR according to standard protocoles (Promega Corp., Madison, WI, USA).…”

Section: Plasmid Constructsmentioning

confidence: 99%

Smad3/AP-1 interactions control transcriptional responses to TGF-β in a promoter-specific manner

et al. 2001

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Smad proteins transduce signals from TGF-b receptors and regulate transcription of target genes either directly or in combination with other sequence-speci®c transcription factors. AP-1 sites and their cognate transcription factors also play important roles in the gene regulatory activities of TGF-b. In this report, we have investigated the functional interactions of the Smad and AP-1 transcription factors. We demonstrate that Smad and AP-1 complexes speci®cally bind to their cognate ciselements and do not interact with each other on-DNA, whereas o -DNA interactions occur between Smad3 and both c-Jun and JunB. Using both arti®cial constructs speci®c for either the Smad or AP-1 signaling pathways or natural promoters known to be TGF-b-responsive, we have determined that Jun family members downregulate Smad3-mediated gene transactivation whereas AP-1-dependent promoters are synergistically activated by Smad3 and Jun proteins. We propose a model where the presence of Smad-and/or AP-1-speci®c cis-elements within TGF-b-responsive genes allows dynamic modulation of gene expression, in contrast to the existing model where interactions between Smad and AP-1 proteins are merely an on/o mechanism to regulate TGF-b/Smad targets. Oncogene (2001) 20, 3332 ± 3340.

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Section: Plasmid Constructsmentioning

confidence: 99%

Smad3/AP-1 interactions control transcriptional responses to TGF-β in a promoter-specific manner

et al. 2001

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“…Commercial Sp1 oligonucleotide and Hela-cell nuclear extract used as positive controls were from Promega. The reactions were stopped by adding 2 µl of loading buffer, loaded on to a 4 % nondenaturing polyacrylamide gel (for oligonucleotides) and electrophoresis conditions were as described in [24]. Gels were pre-run for 30 min at 200 V before loading the samples and running the electrophoresis at 200 V for 2-3 h in 0.5iTris\borate\EDTA buffer at 4 mC.…”

Section: Gel-shift Assaysmentioning

confidence: 99%

Sequence of the 5′-flanking region and promoter activity of the human mucin gene MUC5B in different phenotypes of colon cancer cells

Seuningen¹,

Perrais²,

PIGNY³

et al. 2000

Biochem. J.

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Control of gene expression in intestinal cells is poorly understood. Molecular mechanisms that regulate transcription of cellular genes are the foundation for understanding developmental and differentiation events. Mucin gene expression has been shown to be altered in many intestinal diseases and especially cancers of the gastrointestinal tract. Towards understanding the transcriptional regulation of a member of the 11p15.5 human mucin gene cluster, we have characterized 3.55 kb of the 5h-flanking region of the human mucin gene MUC5B, including the promoter, the first two exons and the first intron. We report here the promoter activity of successively 5h-truncated sections of 956 bases of this region by fusing it to the coding region of a luciferase reporter gene. The transcription start site was determined by primer-extension analysis. The region upstream of the transcription start site is characterized by the presence of a TATA box at bases k32\k26, DNA-binding elements for transcription factors c-Myc, N-Myc, Sp1 and nuclear factor κB as well as putative activator protein (AP)-1-, cAMPresponse-element-binding protein (CREB)-, hepatocyte nuclear

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“…The regulatory regions in desmocollin (type II), 47 desmoglein (type I and III), 48 laminin (B1 chain), 49 matrix metalloproteinase-2 (MMP-2), 50 tissue inhibitor of metalloproteinase-2 (Timp-2), 51 and VEGF 52 genes all contain AP-2 binding motifs, and the laminin (B2 chain) genes also contain binding sites for AP-2-like nuclear protein. 53 Type IV collagen, 54 type VII collagen, 55 desmocollin (type II), 47 MMP-9, 50 Timp-2, 51 and VEGF 52 have also SP-1 binding motifs. NF-B binding motifs have been observed in MMP-9.…”

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confidence: 99%

Differential Expression of Cytokeratin after Orthotopic Implantation of Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability

Morifuji

Taniguchi

Sakai

et al. 2000

The American Journal of Pathology

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Tongue cancer has a high metastatic ability. The survival of cancer patients often depends primarily on whether or not the tumor metastasizes; therefore, it is important to characterize the phenotype of metastatic tumors and to elucidate both the responsible molecules and their functions.Cytokeratins represent important structural components of the epithelial cytoskeleton and thus constitute major protein markers for cellular differentiation.1 They have been widely used as molecular markers in the diagnosis of a variety of carcinomas as well as in the study of many nonmalignant lesions.2 The CK19 fragment, referred to as CYFRA 21-1, has been reported to be useful as an independent prognostic marker of squamous cell carcinoma.3,4 Alterations in the expression patterns of cytokeratins have been reported in carcinoma or carcinoma cell lines using immunohistochemistry, 5,6 Western blot analysis, 5,7 two-dimensional gel electrophoresis, 1,8 and in situ hybridization. 9 However, few reports have analyzed them using metastatic models. It is necessary to analyze cancer cells that have differences in invasive and metastatic abilities to understand the cytokeratin gene regulation as well as the role of cytokeratin in the progression of carcinoma. An orthotopic implantation enabled us to establish the metastatic models. 10,11 In the present study, we examined the relationship between the malignancy and the cytokeratin expression by using two established squamous cell carcinoma cell lines, SQUU-A and SQUU-B, derived from the same human tongue cancer, which demonstrated different invasive and metastatic potentials in the orthotopic implantation system. Materials and Methods Establishment of Cell LinesSQUU-A and SQUU-B cell lines were established from local recurrences of the tongue cancer obtained from a 66-year-old Japanese woman. This tumor had been diagnosed to be a well differentiated squamous cell carcinoma. Surgical specimens were minced with a scalpel into small cubes measuring from 1 to 3 mm in size. These

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A GT-rich Sequence Binding the Transcription Factor Sp1 Is Crucial for High Expression of the Human Type VII Collagen Gene (COL7A1) in Fibroblasts and Keratinocytes

Cited by 39 publications

References 43 publications

Smad3/AP-1 interactions control transcriptional responses to TGF-β in a promoter-specific manner

Smad3/AP-1 interactions control transcriptional responses to TGF-β in a promoter-specific manner

Sequence of the 5′-flanking region and promoter activity of the human mucin gene MUC5B in different phenotypes of colon cancer cells

Differential Expression of Cytokeratin after Orthotopic Implantation of Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability

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