A H<sub>2</sub>O<sub>2</sub>-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2

Xi, Xiaoxue; Wen, Meiqi; Song, Shihao; Zhu, Junlun; Wen, Wei; Zhang, Xun; Wang, Shengfu

doi:10.1039/d0cc01598a

Cited by 34 publications

(15 citation statements)

References 23 publications

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“…The most widely used commercial method is the enzyme-linked immunosorbent assay (ELISA), which requires the utilization of expensive antibody reagents . Because the biological functions of MMPs are mainly dependent on their proteolytic activity, the evaluation of the enzymatic activities of MMPs is more clinically significant. − Thus, a series of cleavage-based methods with substrate peptide-based probes have been presented for the specific interrogation of enzyme activities using colorimetry, , fluorescence, − nanopore, or electrochemical − and eletrochemiluminescence sensors. These strategies generally focus on the assay of single-MMP activity, and the reported multiplex detections with signal tagging at different wavelengths or potentials suffer from the drawbacks of signal overlapping.…”

Section: Introductionmentioning

confidence: 99%

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Liu

Chen

et al. 2022

Anal. Chem.

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This work designed a mass spectrometric biosensing strategy for the multiplex detection of matrix metalloproteinases (MMPs) with a mass-encoded suspension array. This array was fabricated as multiplex sensing probes by functionalizing magnetic beads with MMP-specific peptide-isobaric tags for relative and absolute quantification (iTRAQ) conjugates, which contained a hexahistidine tag for surface binding, a substrate region for MMP cleavage, and a coding region for the specific MMP. The integration of the multiplex coding ability of iTRAQ with ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and the proteolysis method for peptide digestion endowed the biosensing method with high throughput and ultrahigh sensitivity. This strategy could be conveniently performed by mixing the sample and the suspension array for enzymatic reactions and then digesting the uncleaved peptides with trypsin to release the coding regions for UPLC-MS/MS analysis. With MMP-2 and MMP-7 as analytes, the relative changes of peak area ratios of coding regions showed good linear responses in the ranges of 0.2− 100 and 0.5−400 ng mL −1 , with detection limits of 0.064 and 0.17 ng mL −1 , respectively. The analysis of MMP activity in serum samples and its change responding to inhibitors demonstrated the specificity, practicability, and expansibility of the proposed strategy. This work paves a new avenue for the activity assays of multiplex enzymes and promotes the development of mass spectrometric biosensing.

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Section: Introductionmentioning

confidence: 99%

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Liu

Chen

et al. 2022

Anal. Chem.

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“…152,153 To date, researchers have developed a variety of HMNM-based electrochemical, FL and colorimetric biosensors to detect disease-related protein markers, such as tumor, hepatic, cardiac, inflammatory markers, and others (Table 2). 24,30,35,36,46,47,49–52,64,65,81,84,85,88,89,98,101–103,107–110,114,122,154–178…”

Section: Applications Of Hmnms In Analytical Chemistrymentioning

confidence: 99%

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

Wang

Yuan

et al. 2022

Chem. Sci.

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“…Nanozymes also represent an attractive alternative for the attainment of remarkable signal amplification along with stability, easy development at low cost, and resistance to acids and alkalis [73,74]. Inspired by these characteristics, Xi et al [75] fabricated an H 2 O 2 -free peptide-based biosensor for the detection of MMP-2 using a Au@Pt bimetallic nanozyme as a signal amplification label. A peptide including the sequence PLGVR allowed detection of the protease.…”

Section: Peptides As Substrate and Signal Development Agents In Catalytic Biosensorsmentioning

confidence: 99%

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

et al. 2021

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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A H₂O₂-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2

Abstract: We construct for the first time a H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods in neutral substrate solutions for highly sensitive detection of matrix metalloproteinase 2.

Cited by 34 publications

References 23 publications

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

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A H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2

Abstract: We construct for the first time a H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods in neutral substrate solutions for highly sensitive detection of matrix metalloproteinase 2.

Cited by 34 publications

References 23 publications

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

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A H₂O₂-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2