The array of definitions regarding 'peri-urban' areas do not allow the precise measurement of its boundaries in a city. In this study, I developed an easyto-use method to calculate the area where urban and adjacent non-urban systems intermingle. To validate that such areas were ecologically meaningful, I compared bird community species-richness, abundance, and composition from 'intra-' and 'peri-urban' areas in a medium-sized neotropical city. Results show that 'periurban' areas represent an important ecological interaction area for birds, and differ greatly from 'intra-urban' areas. The proposed method is robust and useful for a great variety of amoeboid-growing cities.