A haplotype variant of porcine IFIT2 increases poly(I:C)-induced activation of NF-κB and ISRE-binding factors

Pang, Yu; Zhang, Caixia; Tian, Yange; Song, Yanfang; Liu, Di; Yang, Xiuqin

doi:10.1007/s11033-018-4376-4

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…IFIT2 is an important member of the interferon stimulated gene family that plays a crucial role in the antiviral innate immune response through the inhibition of viral replication ( Chai et al, 2021 ). IFIT2 is not transcribed under basal conditions but is induced to a high level after poly(I:C) treatment ( Pang et al, 2018 ). RUNX2 is an evolutionarily conserved transcription factor that has been described as essential for osteogenesis in the embryonic context, but a recent study revealed the participation of RUNX2 in the antiviral response by modulating long-term memory and the survival and exhaustion of mature T cells ( Mevel et al, 2019 ; Olesin et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation

Zhao

Gan

et al. 2023

PeerJ

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The intestinal epithelium barrier serves as a highly dynamic immunologic frontier in the defense against invading pathogenic bacteria and viruses. Hence, understanding of the complicated underlying relationship between enteric pathogens and the intestinal epithelium barrier is vital for developing strategies to improve the intestinal health of farm animals. To this end, Caco-2 cells were stimulated by 1 µg/ml lipopolysaccharide (LPS) for 24 h and 5 µg/ml polyinosinic-polycytidylic acid (ploy(I:C)) for 4 h to imitate bacterial and viral infection processes, respectively. The specific alterations in gene expression of Caco-2 cells after stimulation were characterized by transcriptome sequencing. Seventy differentially expressed genes (DEGs) were identified under LPS exposure, and 17 DEGs were observed under ploy(I:C) exposure. We found that most DEGs were specific, and only one common DEG SPAG7 was observed. Gene Ontology (GO) annotation analysis indicated that all DEGs identified in the different treatments were mainly derived from GO terms related to the maintenance of cellular homeostasis. Moreover, specific DEGs such as SLC39A10, MT2A, and MT1E regulated by LPS treatment, while IFIT2 and RUNX2 mediated by ploy(I:C) treatment, which are derived from immune function modulation related GO terms, were confirmed by both transcriptome sequencing and qRT-PCR. In addition, both transcriptome sequencing and qRT-PCR results verified that LPS specifically down-regulated the DEGs INHBE and ARF6, which are involved in inflammation responses related to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway including the TGF-beta signaling pathways and the Ras signaling pathway. Ploy(I:C) uniquely suppressed the DEGs GABARAP and LAMTOR3, which participated in viral replication-associated pathways including autophagy and mTOR signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation

Zhao

Gan

et al. 2023

PeerJ

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“…SNHG18 inhibits the nuclear and cytoplasmic transport of ENO1 to promote the occurrence and metastasis of glioma [ 22 ]. IFIT3, a member of IFN-induced protein with tetratricopeptide repeats gene family mediating a variety of protein–protein and protein–RNA interactions, is involved in various cellular regulations [ 23 ]. IFITs are reported as prognostic markers to determine the clinical outcome of many cancers, such as glioblastoma, hepatocellular carcinoma, breast cancer, and pancreatic cancer [ 24 – 27 ].…”

Section: Discussionmentioning

confidence: 99%

FAM64A is an androgen receptor-regulated feedback tumor promoter in prostate cancer

Zhou

et al. 2021

Cell Death Dis

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Endocrine therapy for prostate cancer (PCa) mainly inhibits androgen receptor (AR) signaling, due to increased androgen synthesis and AR changes, PCa evolved into castration-resistant prostate cancer (CRPC). The function of Family With Sequence Similarity 64 Member A (FAM64A) and its association with prostate cancer has not been reported. In our research, we first reported that FAM64A is up-regulated and positively associated with poor prognosis of patients with prostate cancer (PCa) by TCGA database and immunohistochemistry staining. Moreover, knockdown of FAM64A significantly suppressed the proliferation, migration, invasion, and cell cycle of PCa cells in vitro. Mechanistically, FAM64A expression was increased by dihydrotestosterone (DHT) through direct binding of AR to FAM64A promoter, and notably promoted the proliferation, migration, invasion, and cell cycle of androgen-dependent cell line of PCa. In addition, abnormal expression of FAM64A affects the immune and interferon signaling pathway of PCa cells. In conclusion, FAM64A was up-regulated by AR through directly binding to its specific promoter region to promote the development of PCa, and was associated with the immune mechanism and interferon signaling pathway, which provided a better understanding and a new potential for treating PCa.

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A haplotype variant of porcine IFIT2 increases poly(I:C)-induced activation of NF-κB and ISRE-binding factors

Cited by 2 publications

References 32 publications

Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation

Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation

FAM64A is an androgen receptor-regulated feedback tumor promoter in prostate cancer

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