A hierarchical, stretchable and stiff fibrous biotemplate engineered using stagger-electrospinning for augmentation of rotator cuff tendon-healing

Zhao, Song; Zhao, Xin; Dong, Shuang; Yu, Jia; Pan, Guoqing; Zhang, Yang; Zhao, Jinzhong; Cui, Wenguo

doi:10.1039/c4tb01642d

Cited by 31 publications

(23 citation statements)

References 47 publications

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“…Fabrication of the Electrospun Scaffolds : Nanofibrous scaffolds were prepared by electrospinning technique . Briefly, polyvinyl alcohol (degree of polymerization ≈2400–2500; degree of hydrolysis ≈98.0−99.8 mol%) was dissolved in distilled water at a concentration of 8.8 wt%, and chitosan (degree of deacethylation = 90−95%) was dissolved in 1 wt% of acetic acid solution at a concentration of 2 wt% with (PC stand for PVA and chitosan electrospun fiber ) or without (PCD stand for PVA, chitosan, and DFO electrospun fiber) 1 wt% of DFO.…”

Section: Methodsmentioning

confidence: 99%

Upregulating Hif‐1α by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound

Chen

Jia

Kang

et al. 2016

Adv Healthcare Materials

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114

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Nonhealing chronic wounds on foot are one of the most dreaded complications of diabetes, and biomedical scaffolds remain an attractive option for repairing or regenerating tissues. Accelerating angiogenesis in the early stage after injury is critical to wound healing process; however, the scaffolds accelerate the angiogenesis in the beginning but with the acceleration of vessel network formation the scaffold network hinders the process. In this study, the water soluble drugs-loaded hydrogel nanofibrous scaffolds are designed for rapidly recruiting angiogenesis relative cells and promoting wound healing. The sustained release profile of desferrioxamine (DFO), which continues for about 72 h, leads to significantly increase of neovascularization. The majority of the scaffold is degraded in 14 d, leaving enough space for cell proliferation and vessel formation. The in vitro results show that the scaffolds upregulate the expression of Hif-1α and vascular endothelial growth factor, and enhance the interaction between fibroblasts and endothelial cells. The in vivo studies show a higher expression of angiogenesis related cytokines. This study demonstrates that the DFO released from hydrogel nanofibrous scaffolds of quick degradation can interfere with the required prolyl-hydroxylases cofactors by acting as Fe(2+) chelator and upregulate the expression of Hif-1α, leading to a significant increase of the neovascularization.

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Section: Methodsmentioning

confidence: 99%

Upregulating Hif‐1α by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound

Chen

Jia

Kang

et al. 2016

Adv Healthcare Materials

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114

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“…Multiple nozzles from different directions were used concurrently to deposit more than one materials onto the collector. As a composite scaffold, PCL fibres of 1.2 μm and chitosan fibres of 360 nm were co‐electrospun for treatment of rotator cuff tears by Zhao et al (). Interestingly, the composite scaffolds represented remarkably enhanced strength and failure strain than chitosan scaffolds and improved stiffness than PCL scaffolds.…”

Section: Current Fibre‐based Techniques For Tendon Tementioning

confidence: 99%

“…In order to make up for the limitations of a single material, (Czaplewski et al, 2014;Rothrauff et al, 2017), collagen-silk (Chen et al, 2008;Zheng et al, 2017), PCL-chitosan (Domingues et al, 2016;Zhao et al, 2015), PCL-gelatin (Yang, Lin, Rothrauff, Yu, & Tuan, 2016), and PLGA-silk (Sahoo, Ouyang, Goh, Tay, & Toh, 2006).…”

Section: Biomaterials For Tendon Scaffoldsmentioning

confidence: 99%

“…(b, d, f, h, j, l, n) Images of fibre or fibre bundle manufactured using related techniques Costa-Almeida et al, 2017;Kew et al, 2012;Tamura et al, 2002;Wu, Wang, et al, 2015;Yin et al, 2010;Younesi et al, 2014) [Colour figure can be viewed at wileyonlinelibrary.com] more than one materials onto the collector. As a composite scaffold, PCL fibres of 1.2 μm and chitosan fibres of 360 nm were coelectrospun for treatment of rotator cuff tears by Zhao et al (2015).…”

Section: Electrospinningmentioning

confidence: 99%

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Fibre-based scaffolding techniques for tendon tissue engineering

Han

Wong

et al. 2018

J Tissue Eng Regen Med

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Tendon refers to a band of tough, regularly arranged, and connective tissue connecting muscle and bone, transferring strength from muscle to bone, and enabling articular stability and movement. The limitations of natural tendon grafts motivate the scaffold-based tissue engineering (TE) approaches, which aim to build patient-specific biological substitutes that can repair the damaged or diseased tissues. Advances in engineering and knowledge of chemistry and biology have brought forth numerous fibre-based technologies, including electrospinning, electrohydrodynamic jet printing, electrochemical alignment technique, and other fibre-assembly technologies, which enable the fabrication of tendon tissue structure in 3-dimension. Textile techniques such as knitting and braiding have also been performed based on the fibrous materials to produce more complex structure. These scaffolds showed great similarity with native tendons in architectural features, mechanical properties, and facilitate biological functionality such as cellular adhesion, ingrowth, proliferation, and differentiation towards tendon tissue. Herein, we review the techniques that have been used to assemble fibres into scaffolds for tendon TE application. The morphological structures, mechanical properties, materials, degradation characteristics, and biological activities of the induced scaffolds were compared. The existing challenges and future prospects of fibre-based tendon TE have also been discussed.

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“…Designing the hierarchical micro/nanoscaled scaffolds for tissue generation could (1) modulate the functional response of cell; (2) induce structural and functional integration of cells and tissues; (3) control the process of tissue remodeling by the structure and component modulation of hierarchical network. Recently, considerable research efforts have been made to develop various hierarchical micro/nanostructured scaffolds for bone repair, Achilles tendons, rotator cuffs, and skin tissue . Dura mater is a connective tissue membrane composed mainly of hierarchical collagen fibers with various diameters, multilayers, and orientations .…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Micro/Nanofibrous Bioscaffolds for Structural Tissue Regeneration

Cui

Zhang

et al. 2017

Adv Healthcare Materials

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Various biomimetic scaffolds with hierarchical micro/nanostructures are designed to closely mimic native extracellular matrix network and to guide cell behavior to promote structural tissue generation. However, it remains a challenge to fabricate hierarchical micro/nanoscaled fibrous scaffolds with different functional components that endow the scaffolds with both biochemical and physical features to exert different biological roles during the process of tissue healing. In this study, a biomimetic designed micro/nanoscaled scaffold with integrated hierarchical dual fibrillar components is fabricated in order to repair dura mater and prevent the formation of epidural scars via collagen molecule self-assembly, electrospinning, and biological interface crosslinking strategies. The fabricated biomimetic scaffolds display micro/nanofibers staggered hierarchical architecture with good mechanical properties and biocompatibility, and it has a more profound effect on attachment, proliferation, and differentiation of fibroblasts. Using a rabbit duraplasty model in vivo, the authors find that dural defects repaired with hierarchical micro/nanoscaled scaffold form a continuous neodura tissue similar to native dura mater; furthermore, the number of scar tissues decreases significantly in the laminectomy sites compared with conventional electrospun microfibrous scaffold. Taken together, these data suggest that the hierarchical micro/nanoscaled fibrous scaffolds with dual fibrillar components may act as a "true" dural substitutes for dual repair.

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A hierarchical, stretchable and stiff fibrous biotemplate engineered using stagger-electrospinning for augmentation of rotator cuff tendon-healing

Abstract: Engineering hierarchical, stretchable and stiff fibrous biotemplate using stagger-electrospinning for the augmentation of rotator cuff tendon-healing.

Cited by 31 publications

References 47 publications

Upregulating Hif‐1α by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound

Upregulating Hif‐1α by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound

Fibre-based scaffolding techniques for tendon tissue engineering

Hierarchical Micro/Nanofibrous Bioscaffolds for Structural Tissue Regeneration

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