A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat

Boyko, Elena V.; Gill, Kulvinder S.; Mickelson-Young, Leigh; Nasuda, Shuhei; Raupp, W. J.; Ziegle, J.; Singh, Sukhwinder; Hassawi, Dhia S.; Fritz, A. K.; Namuth, Deana; Lapitan, Nora L. V.; Gill, Bikram S.

doi:10.1007/s001220051204

Cited by 71 publications

(45 citation statements)

References 24 publications

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“…Schmal. (Hart 1997;Blanco et al 1998;Boyko et al 1999). However, some loci on these linkage maps were not identified in this study.…”

Section: Resultscontrasting

confidence: 40%

Comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions

Weng¹,

Lazar²

2002

Theor Appl Genet

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Eighty two new loci, mapped with 51 DNA clones, were added to the earlier deletion maps of the homoeologous group-6 short arms of hexaploid wheat ( Triticum aestivum L. em Thell., 2n = 6 x = 42, AABBDD). There are now 41, 56 and 52 loci mapped on deletion maps of 6AS, 6BS and 6DS, respectively. The linear order of orthologous loci in all three arms appears to be identical. The majority of the loci are located in the distal one-half of the three arms. There seems to be an increased marker/gene density from the centromeric to the telomeric regions in each arm, and the marker density in comparable physical regions is similar on all three maps. Recombination is not uniformly distributed along the chromosome arms; 60% of recombination occurs in the distal one-third of each arm. Recombination increases from the proximal region to the distal end in a nonlinear pattern. The distribution of loci and recombination along each of the three chromosome arms is highly correlated. Comparison of the 6BS deletion map from this study and a 6HS physical map of barley ( Hordeum vulgare L., 2n = 2 x = 14, HH) reveals a remarkably similar distribution of recombinogenic and gene-rich regions between the two chromosome arms, suggesting that the distribution patterns of genes may be conserved in the homoeologous group-6 chromosome short arms of wheat and barley. A consensus map of wheat group-6 short arms containing 46 orthologous loci was constructed. Comparison of the consensus map with published linkage maps of Triticeae group-6 chromosome short arms indicates that the linear order of the loci on the maps has been largely conserved. Evidence from this study does not support the existence of a 2BS-6BS reciprocal terminal translocation.

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“…Schmal. (Hart 1997;Blanco et al 1998;Boyko et al 1999). However, some loci on these linkage maps were not identified in this study.…”

Section: Resultscontrasting

confidence: 40%

Comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions

Weng¹,

Lazar²

2002

Theor Appl Genet

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“…The PIC values of SSR loci as genetic markers are also usually directly correlated to the type and number of repeats present. Kapila et al (2008) (Kleinhofs et al, 1993, Boyko et al, 1999, Huang et al, 2002, Ni et al, 2002. In pearl millet, LG 6 having the lowest number (1) of polymorphic SSR markers and low PIC (0.49) were observed in present study.…”

Section: Molecular Marker Analysismentioning

confidence: 53%

Molecular characterization of pearl millet [Pennisetum glaucum (L.) R. Br] inbreds using microsatellite markers

Nehra

Kumar

Vart

et al. 2017

JANS

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Studies on genetic diversity in Pennisetum germplasm are the promising opportunities for the use of undomesticated materials for improving pearl millet varieties. DNA based markers have now emerged as a potential genomic tool for estimation of genetic diversity among various cultivars and varietal identification. In present study, genetic diversity among 49 stay green inbreds of pearl millet was studied using simple sequence repeats (SSRs). Twenty nine polymorphic SSR primers, identified after initial screening of 70, were used to study diversity among these lines. A total of 108 alleles were amplified, collectively yielding unique SSR profiles for all the 49 inbreds. The average number of SSR alleles per locus was 3.72, with a range from 2 to 13. Polymorphic information content (PIC) values of various SSR loci across all the 49 inbreds ranged from 0.14 to 0.87 with an average of 0.51 per locus. This indicated sufficient diversity among the 49 pearl millet inbreds and total 5 out of 29 polymorphic SSR loci, namely Xpsmp2070, Xpsmp2001, Xpsmp2008, Xpsmp2066, Xpsmp2072 revealed PIC values above 0.70, can be considered highly useful for differentiation of pearl millet inbred lines. The lowest PIC value (0.47) for linkage group 7 showed comparatively conserved nature of this linkage group A dendrogram obtained using WARD's minimum variance method further delineates 49 inbreds into 8 major clusters, and the clustering pattern corroborated with their pedigree and characteristics traits.

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“…It is likely that the RWA-resistant gene a F 2 progeny derived from the F 1 wheat plants that were artificially infested with RWA for 20 days (all showed resistant) at the seedling stage b F 2 progeny derived from un-infested F 1 wheat plants c Phenotype or genotype: X 1 X 1 = homozygous resistant, X 2 X 2 = homozygous susceptible, X 1 X 2 = heterozygous (if not detectable, then grouped together with X 1 X 1 ) d P < 0.05 = significant deviation from the expected segregation ratio, P > 0.05 = fit to the expected segregation ratio of the F 2 population promotes gamete competition under the selection pressure of RWA infestation. Significant deviations from expected Mendelian segregation ratios of molecular markers or physical loci have been reported on chromosomes 1D, 3D, 4D, 5D and 7D (near the centromere) in Aegilops tauschii, the diploid D-genome progenitor of bread wheat (Faris et al 1998;Boyko et al 1999). Deviation phenomena and distortion loci have also been reported in other species, and the loci were regarded as segregation distorters, distortion factors, distortion genetic elements, and genetic selection factors (Lyttle 1991;Faris et al 1998), but they have not been identified as associated with genes of specific function.…”

Section: Microsatellite Markers Linked To Dn6mentioning

confidence: 99%

Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6

2002

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The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is a serious economic pest of wheat worldwide. Host plant resistance is the preferred method to control RWA infestations. The identification and mapping of RWA-resistant genes and the development of resistant wheat cultivars can be facilitated through the use of molecular markers. In the present study, microsatellite (SSR) markers linked to the RWA-resistant genes Dn4 and Dn6 were identified using several F(2) mapping populations derived from crosses of susceptible wheat cultivars and resistant sources. Two flanking microsatellite markers Xgwm106 and Xgwm337 are linked in coupling phase with Dn4 on the short arm of wheat chromosome 1D at 7.4 cM and 12.9 cM, respectively. Two other microsatellite markers Xgwm44 and Xgwm111 are linked to Dn6 in coupling phase near the centromere on the short arm of chromosome 7D at 14.6 cM and 3.0 cM, respectively. This is the first report on the chromosomal location of Dn6, which proved to be either allelic or tightly linked to Dn1, Dn2 and Dn5. This result of Dn6 location contradicts previous reports that Dn6 was independent of Dn1, Dn2 and Dn5. The linked markers can be conveniently used for marker-assisted selection in wheat breeding programs for the identification and/or pyramiding of Dn4 and Dn6 genes.

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A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat

Cited by 71 publications

References 24 publications

Comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions

Comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions

Molecular characterization of pearl millet [Pennisetum glaucum (L.) R. Br] inbreds using microsatellite markers

Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6

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