Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6

Liu, X. M.; Smith, C. Michael; Gill, Bikram S.

doi:10.1007/s00122-001-0831-y

Cited by 86 publications

(77 citation statements)

References 31 publications

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“…Another RWA resistance gene, Dn4, was mapped on the short arm of the homoeologous wheat chromosome 1D (Ma et al 1998;Liu et al 2002). Dn7 and Dn4 share a common marker, Xksud14 (Ma et al 1998).…”

Section: Genetics Of Dn7mentioning

confidence: 99%

“…The level of resistance conferred by Dn7 was compared with resistance from two previously characterized genes, Dn4 and Dn2 (Ma et al 1998;Liu et al 2001Liu et al , 2002. '94M370', 'Halt', 'PI262220', and the susceptible cultivars, 'Carson' and 'Gamtoos', were grown in 6-inch pots, with one plant per pot.…”

Section: Type Of Resistance Conferred By Dn7mentioning

confidence: 99%

“…Three modes of inheritance were observed in PI 292994: a single dominant gene, two dominant independent genes, or one dominant and one recessive gene conferring resistance to RWA (Zhang et al 1998). All these resistance genes have been genetically mapped using molecular markers (Ma et al 1998;Myburg et al 1998;Venter and Botha 2000;Liu et al 2001Liu et al , 2002Miller et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat

et al. 2003

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The Russian wheat aphid is a significant pest problem in wheat and barley in North America. Genetic resistance in wheat is the most effective and economical means to control the damage caused by the aphid. Dn7 is a rye gene located on chromosome 1RS that confers resistance to the Russian wheat aphid. The gene was previously transferred from rye into a wheat background via a 1RS/1BL translocation. This study was conducted to genetically map Dn7 and to characterize the type of resistance the gene confers. The resistant line '94M370' was crossed with a susceptible wheat cultivar that also contains a pair of 1RS/1BL translocation chromosomes. The F(2) progeny from this cross segregated for resistance in a ratio of 3 resistant: 1 susceptible, indicating a single dominant gene. One-hundred and eleven RFLP markers previously mapped on wheat chromosomes 1A, 1B and 1D, barley chromosome 1H and rye chromosome 1R, were used to screen the parents for polymorphism. A genetic map containing six markers linked to Dn7, encompassing 28.2 cM, was constructed. The markers flanking Dn7 were Xbcd1434 and XksuD14, which mapped 1.4 cM and 7.4 cM from Dn7, respectively. Dn7 confers antixenosis, and provides a higher level of resistance than that provided by Dn4. The applications of Dn7 and the linked markers in wheat breeding are discussed.

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Section: Genetics Of Dn7mentioning

confidence: 99%

Section: Type Of Resistance Conferred By Dn7mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat

et al. 2003

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“…These genes include Dn1, Dn2, Dn4, Dn5, Dn6, Dn7, Dn8, Dn9, and Dnx (Anderson et al, 2003;Botha et al, 1995;Liu et al, 2001Liu et al, , 2002Miller et al, 2001;Myburg et al, 1998). The use of DNA markers in breeding for RWA resistance provides several advantages including the ability to screen year-round instead of only during cooler months of the year when the RWA multiplies, and ability to breed for resistance in places where the aphid does not yet occur.…”

Section: Introductionmentioning

confidence: 97%

DNA and morphological markers for a Russian wheat aphid resistance gene

2004

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The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is a significant insect pest of wheat worldwide. Morphological and molecular markers associated with RWA resistance could be used to increase the accuracy and efficiency of selection of resistant germplasm and facilitate transfer to desirable wheat genotypes. The objective of this work was to identify microsatellite (SSR) markers linked to the RWA resistance gene (Dn4) and glume-colour gene (Rg2) using a population of F 2 -derived F 3 families originating from a cross between a susceptible line (synthetic hexaploid-11) and a resistance cultivar (Halt). Two microsatellite markers Xgwm106 and Xgwm337 flanked Dn4 on the short arm of chromosome 1D at 5.9 and 9.2 cM, respectively. Two other microsatellite markers, Xpsp2999 and Xpsp3000, at the distal part of this chromosome arm are linked to Dn4 and to Rg2. The accuracy and efficiency of marker-assisted selection were calculated for homozygous Dn4Dn4 genotypes in the F 2 generation. The gene Rg2 for red glume colour can also be used for marker-assisted selection of Dn4 gene individually and in combination with microsatellite markers. When used together, the closest markers Xgwm106 and Xgwm337, provide 100% accuracy and 75% efficiency. One hundred percent accuracy is also achieved when the morphological marker red glume is used in combination with either Xgwm106 or Xgwm337. Using these flanking markers, it may be possible to fix resistance to RWA in the first segregating generation of an F 2 population without infestation with aphids.

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“…Each PCR reaction mixture contained 12.5 μl master mix, 50 ng template DNA, and 150 ng each of left and right flanking primers in a total volume of 25 μl. The PCR conditions were as described by Liu et al (2002). Amplified products were separated in 3% agarose gels at 6 V/cm in 1× TBE buffer.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Genetic analysis and molecular mapping of a wheat gene conferring tolerance to the greenbug (Schizaphis graminum Rondani)

et al. 2004

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The greenbug, Schizaphis graminum (Rondani), is one of the major pests of wheat worldwide. The efficient utilization of wheat genes expressing resistance to greenbug infestation is highly dependent on a clear understanding of their relationships. The use of such genes will be further facilitated through the use of molecular markers linked to resistance genes. The present study involved several F(2) wheat populations derived from crosses between susceptible cultivars and resistant germplasm carrying different greenbug resistance genes. These populations were used to characterize the inheritance of a wheat gene ( Gbz) conferring tolerance to greenbug biotype I, to identify molecular markers linked to Gbz, and to investigate the relationship between Gbz and Gb3, a previously identified greenbug resistance gene. Our results indicated that Gbz is inherited as a single dominant gene. Microsatellite marker Xwmc157 is completely linked to Gbz, and Xbarc53 and Xgdm46 flank Gbz at distances of 5.1 and 9.5 cM, respectively. Selection of Gbz using marker Xwmc157 alone gives breeders 100% selection accuracy. Gbz may be placed in the distal region of the long arm of the wheat chromosome 7D. The results of allelism tests indicated that Gbz is either allelic or tightly linked to Gb3.

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Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6

Cited by 86 publications

References 31 publications

Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat

Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat

DNA and morphological markers for a Russian wheat aphid resistance gene

Genetic analysis and molecular mapping of a wheat gene conferring tolerance to the greenbug (Schizaphis graminum Rondani)

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