A High Frequency Dielectrophoresis Cytometer for Continuous Flow Biological Cells Refinement

Abstract

This paper presents a microfluidic radiofrequency device operating at a few hundred MHz, which is able to sort biological cells. It uses a non-invasive and label-free technic based on intracellular dielectric specificities of biological cells. The sorting principle relies on a dynamic dielectrophoresis (DEP) deviation resulting from the interaction between a high frequency electric signal and the cell cytoplasm content. Driven in a microfluidic channel by a continuous flow, cells are individually deflected from their primary trajectories after having entered a non-uniform electric field generated by a microelectrode system. Designed with different slopes, these electrodes allow a selective guiding of cells to different outlets depending on the dielectrophoresis deviation efficiency. To allow a successful cell sorting, the intensity of deviation forces acting on cells is modulated according to the particle speed, the dielectrophoresis signal frequency and the electrode slope angles related to the Clausius-Mossotti factor of each cell. As proof of concept, experiments with cells from glioblastoma line were carried out, using different DEP signal frequencies to highlight system ability to sort cells from heterogeneous basal population into less disparate sub-populations.

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

“…UHF-DEP is a very promising tool with great potential to discriminate cells according to their internal biological properties. Hence, from the identification of UHF-DEP signa-tures, we can see the perspective to develop a cell sorting device for isolating cancer stem cells [31]. In the future, the early detection of CSC subpopulation in a glioma tumor with a UHF-DEP approach could have a prognosis value on therapeutic response and might allow adaption of a therapeutic strategy following diagnosis.…”

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

“…UHF-DEP is a very promising tool with the great potential to discriminate cell according to their internal biological properties. Hence, from the identification of UHF-DEP signatures, we can see the perspective to develop a cell sorting device for isolating cancer stem cells [30]. In the future, the early detection of CSC subpopulation in glioma tumor…”

Lab-On-Chip UHF-Dielectrophoretic Cytometer for Colorectal Cancer Stem Cells Sorting

“…To do so, a dedicated electrode has been designed with three different sections with increasing slopes (35°, 45° and 60°). Hence, such UHF-DEP cytometer would be able to separate a cell suspension into four subpopulations driven at the end of the device where four outlets have been implemented to collect sorted cells [19].…”