A high-level and regulatable production system for recombinant glycoproteins using a human interferon-α promoter-based expression vector

Mori, Tetsuya; Yamamoto, K.; Ohta, Toshio; Sakamoto, Chieko; Sato, Mamoru; Koide, Kazunori; T, Murakami; Fujii, Mitsukiyo; Fukuda, Shigeharu; Kurimoto, Masashi

doi:10.1016/0378-1119(94)90392-1

Cited by 7 publications

(1 citation statement)

References 12 publications

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“…A similar phenomenon is also observed in the LNK catalyst, which is illustrated in Figure for different catalyst loadings. The addition of eutectic catalysts to the raw coal causes some possible interaction between the alkali metals in the eutectic catalyst with the surface functional groups on the carbon during pyrolysis . Such surface interaction is responsible for the pores on the coal surface being covered in these samples.…”

Section: Resultsmentioning

confidence: 99%

Distribution and Catalytic Activity of Eutectic Salts in Steam Gasification of Coal

Godavarty

Agarwal

2000

Energy Fuels

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The reactivity of coal char in steam is enhanced by the addition of binary and ternary eutectic alkali salts due to their reduced melting points. The catalytic steam gasification of Illinois No. 6 coal was carried out using binary (Na2CO3−K2CO3) and ternary (Li2CO3−Na2CO3−K2CO3) eutectic salt mixtures in a fixed bed reactor at different catalyst loadings. For the same catalyst loading, the ternary catalyst showed higher carbon conversions compared to the binary catalyst. The physical distribution of both catalyst systems on the coal char surface was examined using a scanning electron microscope (SEM). Both catalysts showed uniform dispersion on the ungasified coal matrix indicating homogeneous physical mixing. An attempt has been made to establish a qualitative correlation between the type of catalyst, its distribution, and effect on the overall carbon conversions. Gasified chars were highly porous in nature and coalesce in the case of the binary catalyst whereas the ternary catalyst showed highly porous and crystalline morphology. X-ray diffraction spectra showed the formation of potassium polysulfides (K x S y ) in the gasified chars of the eutectic catalyst systems.

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Section: Resultsmentioning

confidence: 99%

Distribution and Catalytic Activity of Eutectic Salts in Steam Gasification of Coal

Godavarty

Agarwal

2000

Energy Fuels

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Components of Vectors for Gene Transfer and Expression in Mammalian Cells

Makrides

1999

Protein Expression and Purification

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Coculture of hepatic and renal origin cell lines provides biohemofiltration with an active transport system of xenobiotics metabolites

et al. 2001

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To improve the present bioartificial liver systems by adding the capacity for selective and active transport, recombinant hepatic GS-HepG2 and renal proximal convoluted tubular cell line-multidrug resistant (PCTL-MDR) cell lines were cocultured on opposite sides of a expanded polytetrafluoroethylene (ePTFE) membrane, and active transport and drug metabolism were examined. PCTL-MDR formed a tight monolayer on the ePTFE membrane to block nonspecific leakage of polysaccharide, while the cells transported anionic digoxin from the membrane side to the medium side. GS-HepG2 cells showed a capacity for testosterone metabolism, and the metabolite, probably androstenedione, as well as testosterone, was transported by PCTL-MDR. The combination of hepatic and renal cell lines in the appropriate bioreactor vessel will enable bioartificial hemofiltration, which has not been achieved with the present bioartificial liver assist systems.

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A high-level and regulatable production system for recombinant glycoproteins using a human interferon-α promoter-based expression vector

Cited by 7 publications

References 12 publications

Distribution and Catalytic Activity of Eutectic Salts in Steam Gasification of Coal

Distribution and Catalytic Activity of Eutectic Salts in Steam Gasification of Coal

Components of Vectors for Gene Transfer and Expression in Mammalian Cells

Coculture of hepatic and renal origin cell lines provides biohemofiltration with an active transport system of xenobiotics metabolites

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