A high performance liquid chromatography method for simultaneous determination of rosiglitazone and gemfibrozil in human plasma

Validation of an LC/MS method for the determination of gemfibrozil in human plasma and its application to a pharmacokinetic study

“…These five validation runs yielded lines with an average slope of 0.133 ± 0.0046 (mean ± SD, n=5) and a y-intercept of −0.010 ± 0.0049 (mean ± SD, n=5) with a precision of 3.46% (Table 1). The LLOQ of the assay was 0.5 μg/mL, which is consistent with sensitivities previously reported for HPLC-UV and fluorescence methods (Schneck et al, 2004; Randinitis et al, 1986; Kang et al, 2009; Lilja et al, 2005). No carryover was noted in the studied GFZ concentration range, as blank samples injected after high standard injection showed no detectable peak.…”

“…To date, the majority of papers reporting bioanalytical methods for the quantification of GFZ in plasma have used HPLC-UV (sensitivities ranging from 0.05 μg/ml to 1 μg/ml) and HPLC-fluorescence (sensitivities ranging from 0.01 μg/ml to 0.5 μg/ml) (Hengy et al, 1985; Randinitis et al, 1986; Forland et al, 1987; Nakagawa et al, 1991; Hermening et al, 2000; Gonzalez-Penas et al, 2001; Niemi et al, 2003; Schneck et al, 2004; Deng et al, 2005; Vittal et al, 2006; Kang et al, 2009). Fluorescence and UV detection methods have long run times (in some cases up to 20 minutes), and require extensive sample preparation techniques (Forland et al, 1987; Nakagawa et al, 1991; Hermening et al, 2000; Vittal et al, 2006; Kang et al, 2009).…”

“…Fluorescence and UV detection methods have long run times (in some cases up to 20 minutes), and require extensive sample preparation techniques (Forland et al, 1987; Nakagawa et al, 1991; Hermening et al, 2000; Vittal et al, 2006; Kang et al, 2009). Tandem mass spectrometry (LC-MS/MS) bioanalytical methods have been reported for GFZ, with assay sensitivities ranging from 0.001 μg/ml to 0.1 μg/ml and shorter run times (e.g., 2 minutes) (Roadcap et al, 2003; Tornio et al, 2008; Busse et al, 2009).…”

Development and validation of a UPLC–MS/MS method for quantification of SKLB010, an investigational anti-inflammatory compound, and its application to pharmacokinetic studies in beagle dogs

“…Several methods have been reported for the detection of thiazolidinedione drugs in biological fluids, such as high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [3], fluorescence detection [4,5] and capillary zone electrophoresis [6]. In our early studies, we utilized HPLC with ultra-violet (UV) detection to quantify SKLB010 in biological matrices.…”

Development and Validation of a Rp- HPLC Method for Estimation of Rosiglitazone in Bulk and Tablet Dosage Form

“…Rosiglitazone is an oral antidiabetic agent and acts as an agonist at PPAR gamma receptors have acts primarily enhances tissue sensitivity to insulin. A literature survey reveals that various analytical methods like rosiglitazone by HPLC and MECK 2 , Simple HPLC method for the determination of rosiglitazone in human plasma 3,4 , Simultaneous HPLC estimation of metformin in combination with rosiglitazone 5 ,rosiglitazone with gliclazide in tablet 6 , rosiglitazone and gemfibrozil in human plasma 7 . Simultaneous LC-UV estimation of rosigliazone and glimepiride in plasma 8 , rosigliazone and glimepiride in human plasma 9 .…”