A simple high-performance liquid chromatographic (HPTLC) method has been developed and validated for simultaneous determination of pioglitazone and glimepiride in bulk and tablet dosage form. The method employed TLC aluminium plates precoated with silica gel 60 F 254 as the stationary phase. The mobile phase used was a mixture of Benzene: Ethyl acetate: Diethyl ether 6:3:1 v/v. The detection of spot was carried out at 254 nm. The calibration curve of pioglitazone was found to be linear between response was determined of 600 ng/ml to 3600 ng/ml with regression coeficent 0.9984 and calibration curve of glimepiride was found to be linear between 200-1200 ng/ml for glimepiride with regression coefficient of 0.9991. The limit of detection was 57.22 ng/ml and 16.67 ng/ml and the quantification limit was 190.73 ng/ml and 55.58 ng/ml for pioglitazone and glimepiride respectively.The proposed method can be successfully used to determine the drug content of marketed formulation. The accuracy of the proposed method was determined by recovery studies and found to be 97.84 to 99.07 %. The proposed method is applicable to routine analysis of Pioglitazone in bulk and pharmaceutical formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection, limits of quantification, range and solution stability.

A simple, accurate, precise, and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Emtricitabine in capsule dosage forms. The method employed TLC aluminium plates precoated with silica gel 60 F 254 as the stationary phase. The mobile phase used was a mixture of (Chloroform: Methanol 8.5: 1.5v/v). The detection of spot was carried out at 275nm. The calibration curve was found to be linear between 200 to 2200 ng mL -1 with regression coefficient of 0.9992. The proposed method can be successfully used to determine the drug content of marketed formulation. The accuracy of the proposed method was determined by recovery studies and found to be 98.79 to 99.61 %. The proposed method is applicable to routine analysis of Emtricitabine in bulk and pharmaceutical formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection, limits of quantification, range and solution stability.

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated of rosiglitazone in bulk and tablet dosage form. Chromatographic analysis was performed on a C 18 column (250x 4.6 mm, 5µm) with a mixture of Ammonium dihydrogen phosphate buffer (pH 4.5): Acetonitrilein the ratio 65:35 as mobile phase, at a flow rate of 1.0 mL min −1 . UV detection was performed at 230 nm. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The retention times of rosiglitazone was found to be 7.19±0.145 respectively. Linearity was obsered in concentration ranges of 12-70 µg mL −1 . The limit of detection was 0.725 and the quantification limit was 2.41 µg/ml. The accuracy of the proposed method was determined by recovery studies and found to be 98.26% to 101.37%. Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of rosiglitazone and in bulk and tablet dosage form.

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of pioglitazone and metformin in bulk and tablet dosage form. Chromatographic analysis was performed on a C 18 column (250x 4.6 mm, 5µm) with a mixture of Methanol:Phosphate buffer in in the ratio 68:32 as mobile phase, at a flow rate of 1.0 mL min -1 . UV detection was performed at 260 nm. The method was validated for accuracy,precision,specificity,linearity, and sensitivity. The retention times of pioglitazone and metformin were 7.24±0.051 and 2.54±0.038 min respectively. Calibration plots were linear over the concentration ranges 10-35 µg mL -1 and 15-40 µg mL -1 for pioiglitazone and metformin respectively. The Limit of detection was 0.382 and 0.131 µg/ml and the quantification limit was 1.27 µg/ml and 0.436 µg/ml for metformin and pioglitazone respectively. The accuracy of the proposed method was determined by recovery studies and found to be 98.65% to 98.90%. Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of rosiglitazone and metformin in bulk and tablet dosage form.

A simple, accurate, precise, and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Abacavir in tablet dosage forms. The method employed TLC aluminium plates precoated with silica gel 60 F 254 as the stationary phase. The mobile phase used was a mixture of (Chloroform: Methanol 9: 1 v/v). The detection of spot was carried out at 284nm. The calibration curve was found to be linear between 400 to 2400 ng mL-1 with regression coefficient of 0.9992. The proposed method can be successfully used to determine the drug content of marketed formulation. The accuracy of the proposed method was determined by recovery studies and found to be 99.49 to 99.79 %. The proposed method is applicable to routine analysis of Abacavir in bulk and pharmaceutical formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection and limits of quantification.