Validated HPTLC Method for the Determination of Abacavir as Bulk Drug and In Pharmaceutical Dosage Form

Abstract

A simple, accurate, precise, and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Abacavir in tablet dosage forms. The method employed TLC aluminium plates precoated with silica gel 60 F 254 as the stationary phase. The mobile phase used was a mixture of (Chloroform: Methanol 9: 1 v/v). The detection of spot was carried out at 284nm. The calibration curve was found to be linear between 400 to 2400 ng mL-1 with regression coefficient of 0.9992. The proposed method can be successfully used to determine the drug content of marketed formulation. The accuracy of the proposed method was determined by recovery studies and found to be 99.49 to 99.79 %. The proposed method is applicable to routine analysis of Abacavir in bulk and pharmaceutical formulations. The proposed method was validated according to various ICH parameters like linearity, accuracy, precision, specificity, limits of detection and limits of quantification.