A high sensitive and specific QDs FRET bioprobe for MNase

Huang, Shan; Xiao, Qi; Zhi, He; Liu, Yi; Tinnefeld, Philip; Su, Xiong; Xiao, Peng

doi:10.1039/b815061c

Cited by 86 publications

(45 citation statements)

References 34 publications

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“…With the addition of DNase I, FRET decreased from digestion of the duplex DNA and loss of the FRET acceptor. Huang et al reported a similar strategy for sensing micrococcal nuclease (MNase) activity (Huang et al 2008). Streptavidin-coated CdSe/ZnS QD550 was assembled with a DNA probe that was biotinylated at one terminus and labeled with 6-carboxy-X-rhodamine (ROX) at the opposite terminus.…”

Section: Nuclease Assaysmentioning

confidence: 99%

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

Wu¹,

Algar²

2015

Nanobiosensors and Nanobioanalyses

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Semiconductor quantum dots (QDs) are very promising materials for optical sensing and bioanalysis. This chapter builds on Chap. 10, which reviewed the optical properties of QDs and their benefits for energy transfer, by illustrating the utility of QDs and energy transfer for optical sensing and bioanalysis. Representative examples of different in vitro assays and cellular probes from the literature are described. Energy transfer mechanisms including Förster resonance energy transfer (FRET), bioluminescence and chemiluminescence resonance energy transfer (BRET and CRET), nanosurface energy transfer (NSET), and charge transfer can be used for optical signal generation in homogeneous assays, single-particle assays, and heterogeneous assays targeting bioanalytes as diverse as nucleic acids, proteins, small molecules, ions, and the activity of enzymes such as proteases, kinases, and nucleases. The importance and versatility of QDs in optical sensing and bioanalysis has been growing steadily since their introduction and will continue to grow in the near future as QD-based assays are optimized and applied to new problems, and new capabilities are developed.

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Section: Nuclease Assaysmentioning

confidence: 99%

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

Wu¹,

Algar²

2015

Nanobiosensors and Nanobioanalyses

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“…CdSe/ZnS-Ab complex was prepared similar to the previous report [27]. Anti-ABA antibody used in the reaction was in 6 times excess over CdSe/ZnS.…”

Section: Preparation Of Cdse/zns-antibody (Cdse/zns-ab) Complexmentioning

confidence: 99%

Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid

et al. 2011

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Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples. abscisic acid, immunomagnetic assay, chemiluminescence, CdSe/ZnS

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“…The hydrophobic CdSe/ZnS QDs were prepared according to the method described previously [35]. In a typical synthesis, 0.0254 g CdO and 0.2280 g stearic acid were put into a three-neck flask.…”

Section: Preparations Of Hydrophobic Cdse/zns Qdsmentioning

confidence: 99%

A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots

Huang

Guan

et al. 2009

Analytica Chimica Acta

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102

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A high sensitive and specific QDs FRET bioprobe for MNase

Cited by 86 publications

References 34 publications

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid

A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots

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