A High-Sensitivity Potentiometric 65-nm CMOS ISFET Sensor for Rapid E. coli Screening

Jiang, Yu; Liu, Xu; Dang, Tran Chien; Huang, Xiwei; Feng, Hao; Zhang, Qing; Yu, Hao

doi:10.1109/tbcas.2018.2793861

Cited by 58 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a result, ISFETs are uniquely suited to address and bridge major limitations in the area of electrochemical instrumentation offering sensor miniaturization, scalability, mass fabrication and low cost. Numerous sensor architectures have appeared in the literature which leverage on the coexistence of sensors and instrumentation on the same IC to facilitate the transition from System to Lab-on-Chip (LoC) [3]- [6]. Recently, ISFETbased LoC platforms have been used to address the expanding field of rapid diagnostics with applications in infectious diseases [7], cancer [8], SNP identification [9] and multi-ion detection [10].…”

Section: Introductionmentioning

confidence: 99%

A 12.8 k Current-Mode Velocity-Saturation ISFET Array for On-Chip Real-Time DNA Detection

Miscourides

Rodríguez-Manzano

et al. 2018

IEEE Trans. Biomed. Circuits Syst.

View full text Add to dashboard Cite

This paper presents a large-scale CMOS chemical-sensing array operating in current mode for real-time ion imaging and detection of DNA amplification. We show that the current-mode operation of ion-sensitive field-effect transistors in velocity saturation devices can be exploited to achieve an almost perfect linearity in their input-output characteristics (pH-current), which are aligned with the continuous scaling trend of transistors in CMOS. The array is implemented in a 0.35-m process and includes 12.8 k sensors configured in a 2T per pixel topology. We characterize the array by taking into account nonideal effects observed with floating gate devices, such as increased pixel mismatch due to trapped charge and attenuation of the input signal due to the passivation capacitance, and show that the selected biasing regime allows for a sufficiently large linear range that ensures a linear pH to current despite the increased mismatch. The proposed system achieves a sensitivity of 1.03 A/pH with a pH resolution of 0.101 pH and is suitable for the real-time detection of the NDM carbapenemase gene in E. Coli using a loop-mediated isothermal amplification.

show abstract

Section: Introductionmentioning

confidence: 99%

A 12.8 k Current-Mode Velocity-Saturation ISFET Array for On-Chip Real-Time DNA Detection

Miscourides

Rodríguez-Manzano

et al. 2018

IEEE Trans. Biomed. Circuits Syst.

View full text Add to dashboard Cite

show abstract

“…Fig. 15 shows the array output and pixel spread after offset cancellation due to row and ADC mismatch, using the same method as stated in [22]. There exists a variation in the ADC output image due to the IR drop across the chip since the chip drains high current.…”

Section: Characterization and Demonstration Of Ion Imagingmentioning

confidence: 99%

“…ISFET arrays are typically used for low frequency biomedical applications such as DNA amplification detection [4] with low requirements on speed and resolution. Other applications reported in the literature include, but are not limited to, monitoring of enzyme kinetics [21], food safety [22] and recording of neural activity [23]. However, we anticipate that there is significant unexplored potential in the area of chemical sensing for a high speed and high resolution ion imager that is able to visualize fast chemical reactions in real-time with integrated on-chip compensation techniques.…”

Section: Introductionmentioning

confidence: 99%

A 128 × 128 Current-Mode Ultra-High Frame Rate ISFET Array With In-Pixel Calibration for Real-Time Ion Imaging

Zeng

Kuang

Miscourides

et al. 2020

IEEE Trans. Biomed. Circuits Syst.

View full text Add to dashboard Cite

An ultra-high frame rate and high spatial resolution ion-sensing Lab-on-Chip platform using a 128 × 128 CMOS ISFET array is presented. Current mode operation is employed to facilitate high-speed operation, with the ISFET sensors biased in the triode region to provide a linear response. Sensing pixels include a reset switch to allow in-pixel calibration for nonidealities such as offset, trapped charge and drift by periodically resetting the floating gate of the ISFET sensor. Current mode row-parallel signal processing is applied throughout the readout pipeline including auto-zeroing circuits for the removal of fixed pattern noise. The 128 readout signals are multiplexed to eight high-sample-rate on-chip current mode ADCs followed by an off-chip PCIe-based readout system on a FPGA with a latency of 0.15 s. Designed in a 0.35 µm CMOS process, the complete system-on-chip occupies an area of 2.6 × 2.2 mm 2 with a pixel size of 18 × 12.5 µm 2 and the whole system achieves a frame rate of 3000 fps which is the highest reported in the literature for ISFET arrays. The platform is demonstrated in the application of real-time ion-imaging through the high-speed visualization of sodium hydroxide (NaOH) diffusion in water at 60 fps on screen in addition to slow-motion playback of ion-dynamics recorded at 3000 fps. Index Terms-ISFET, ultra-high frame rate, linearity conversion, current mode, in-pixel calibration. Junming Zeng (S'17) received the Bachelor degree with first class honors in Electronic Engineering from the University of Southampton, UK in 2016, and the Master degree with Distinction in Analogue and Digital Integrated Circuit Design from Imperial College London, UK in 2017. He is currently a PhD student with Centre for Bio-Inspired Technology, Department of Electrical and Electronic Engineering, Imperial College London UK. His research interests include Analogue and Mixed Signal IC design and digital system design for biomedical applications. Specifically, he is now working on designing a CMOS Labon-Chip ultra-high speed ion imaging platform. He is the recipient of the Department PhD Scholarship from Imperial College London, and the awardee of the Best Student Paper Award 1st Prize at ISCAS 2018. Lei Kuang received the B.Sc. degree in Industrial electronics and control engineering from the Liverpool John Moores University, Liverpool, UK in 2016 and the M.Sc. degree in embedded systems from the University of Southampton, Southampton, UK in 2017. Then, he spent one year working as an embedded system design engineer in China's IC industry for SoC IP design and FPGA development. He is currently working toward his second M.Sc. degree in analog and digital integrated circuit design at Imperial College London, London, UK. His interests include high-speed and low-power digital system design, image processing and algorithm acceleration on FPGAs.

show abstract

“…In pH sensing ISFETs the gate insulator is made of amphoteric materials such as silicon nitride (Si 3 N 4 ). There, in the solution/transistor interface, the following equilibria are being established 24 : …”

Section: Introductionmentioning

confidence: 99%

Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor

Kotsakis

Miliotis

Tzelepi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The timely and accurate detection of carbapenemase-producing Enterobacterales (CPE) is imperative to manage this worldwide problem in an effective fashion. Herein we addressed the question of whether the protons produced during imipenem hydrolysis could be detected using an ion sensitive field effect transistor (ISFET). Application of the methodology on enzyme preparations showed that the sensor is able to detect carbapenemases of the NDM, IMP, KPC and NMC-A types at low nanomolar concentrations while VIM and OXA-48 responded at levels above 100 nM. Similar results were obtained when CPE cell suspensions were tested; NDM, IMP, NMC-A and KPC producers caused fast reductions of the output potential. Reduction rates with VIM-type and especially OXA-48 producing strains were significantly lower. Based on results with selected CPEs and carbapenemase-negative enterobacteria, a threshold of 10 mV drop at 30 min was set. Applying this threshold, the method exhibited 100% sensitivity for NDM, IMP and KPC and 77.3% for VIM producers. The OXA-48-positive strains failed to pass the detection threshold. A wide variety of carbapenemase-negative control strains were all classified as negative (100% specificity). In conclusion, an ISFET-based approach may have the potential to be routinely used for non OXA-48-like CPE detection in the clinical laboratory.

show abstract

A High-Sensitivity Potentiometric 65-nm CMOS ISFET Sensor for Rapid E. coli Screening

Cited by 58 publications

References 36 publications

A 12.8 k Current-Mode Velocity-Saturation ISFET Array for On-Chip Real-Time DNA Detection

A 12.8 k Current-Mode Velocity-Saturation ISFET Array for On-Chip Real-Time DNA Detection

A 128 × 128 Current-Mode Ultra-High Frame Rate ISFET Array With In-Pixel Calibration for Real-Time Ion Imaging

Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor

Contact Info

Product

Resources

About