A high-throughput assay of NK cell activity in whole blood and its clinical application

Lee, Saet byul; Cha, Jeong-Won; Kim, Im-kyung; Yoon, Jae-Cheol; Lee, Hyo Joon; Park, Sang Woo; Cho, Sunjung; Youn, Dong Ye; Lee, Heyja; Lee, Choong Hwan; Lee, Jae Myun; Lee, Kang Young; Kim, Jongsun

doi:10.1016/j.bbrc.2014.02.040

Cited by 60 publications

(82 citation statements)

References 26 publications

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“…NK cell activity was measured using the NK‐Vue Kit ® (ATgen, Sungnam, Korea). A total of 1 mL of whole blood was collected from all participants and placed in an NK‐Vue Tube ® , which is a vacuum filter containing Promoca ® .…”

Section: Methodsmentioning

confidence: 99%

“…After centrifugation at 16,000 r.c.f. for >1 min, the supernatant was collected in another 1.5 mL tube, and sent to enzyme‐linked immunospecific assay plates to measure and quantify IFN‐γ levels released from NK cells.…”

Section: Methodsmentioning

confidence: 99%

“…The chronic inflammatory state in diabetes patients might be involved in the impaired immune function and, consequently, the higher susceptibility to infection. Natural killer (NK) cell activity, in particular, has been shown to be involved in the immune dysfunction and increased risk of cancer in diabetes patients.…”

Section: Introductionmentioning

confidence: 99%

“…NK cells, which strongly express cytokines, have strong cytotoxicity to target cells, whereas cells that have degraded cytokine expression have little or none of this function. A method was recently developed to easily assess the activity of NK cells by measuring the level of secreted interferon‐gamma (IFN‐γ) derived from NK cells. NK cell activity, expressed as a concentration of serum IFN‐γ, is directly related to changes in the function and level of NK cells, and can indicate the degree of innate and adaptive immunity.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between natural killer cell activity and glucose control in patients with type 2 diabetes and prediabetes

Kim

Park

Lee

et al. 2019

J of Diabetes Invest

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Aims/Introduction Natural killer (NK) cells are cytotoxic lymphocytes critical to human immunity. Previous studies showed correlations between NK cell function and blood glucose concentrations. The purpose of the present study was to assess the NK cell activity and various metabolic parameters in people with type 2 diabetes, prediabetes and normal glucose tolerance. Materials and Methods A total of 49 participants were enrolled in the study. Anthropometric and biochemical parameters including age, sex, body mass index, smoking status, blood pressure, fasting plasma glucose, C‐peptide, insulin, glycated hemoglobin, total cholesterol, triglyceride, high‐density lipoprotein cholesterol and low‐density lipoprotein cholesterol were assessed. The 75 g oral glucose tolerance test was carried out for 2‐h postload glucose level. Homeostatic model assessment was calculated for insulin resistance and β‐cell function. NK cell activity was measured by detecting the circulating interferon‐gamma level secreted from NK cells. Results NK cell activity was lower in patients with type 2 diabetes (768.01 ± 650.35) compared with those with prediabetes (2,396.08 ± 653.76, P < 0.001) and normal glucose tolerance (2,435.31 ± 633.22, P < 0.001). In patients with type 2 diabetes, there was a significant inverse linear relationship between NK cell activity and fasting plasma glucose, glycated hemoglobin, and 2‐h postload glucose level (all P < 0.001). Multiple regression analysis showed glycated hemoglobin to be an independent predictor of NK cell activity in patients with type 2 diabetes. Conclusions Compared with individuals with normal glucose tolerance or prediabetes, type 2 diabetes patients have a reduced NK cell activity, and it is significantly related to glucose control.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Relationship between natural killer cell activity and glucose control in patients with type 2 diabetes and prediabetes

Kim

Park

Lee

et al. 2019

J of Diabetes Invest

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“…To overcome these difficulties, a simple assay that uses whole blood instead of PBMC or isolated NK cells was recently developed for commercial use, to measure the activity of NK cells (NK Vue ® Kit; ATGen, Seongnam, Korea) [4,17]. This novel assay employs the serum of ex vivo stimulated whole blood to detect the IFN-γ secreted from the NK cells as an indicator of NK cell activity [4,17]. We aimed to evaluate the association of NK cell activity with the number of cigarettes smoked as well as smoking status, in a very large sample of asymptomatic examinees, using this novel and simple blood test.…”

Section: Introductionmentioning

confidence: 99%

Impact of Smoking on Human Natural Killer Cell Activity: A Large Cohort Study

Jung

Park

et al. 2020

J Cancer Prev

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Some studies have reported a decrease in the natural killer (NK) cell activity in smokers. However, large-scale data on the relationship between NK cell activity and smoking are unavailable. A cross-sectional study was performed on 12,249 asymptomatic examinees who underwent an NK cell activity test, between January 2016 and May 2017. The test quantitated the amount of interferon-γ secreted into the plasma by NK cells, using a patented stimulatory cytokine. The mean age of the study population was 39.1 years, and the proportions of "never", "former", and "current" smokers were 65.5%, 20.9%, and 13.6%, respectively. Current smokers (1,422 pg/mL) had a lower median level of NK cell activity than never smokers (1,504 pg/mL, P = 0.039) and former smokers (1,791 pg/ mL, P < 0.001). Among current smokers, NK cell activity decreased with increase in the number of cigarettes smoked among cur

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Association of neutrophil‐to‐lymphocyte ratio and natural killer cell activity revealed by measurement of interferon‐gamma levels in a healthy population

Kim¹,

Chun

Cho

et al. 2018

Clinical Laboratory Analysis

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A high-throughput assay of NK cell activity in whole blood and its clinical application

Cited by 60 publications

References 26 publications

Relationship between natural killer cell activity and glucose control in patients with type 2 diabetes and prediabetes

Relationship between natural killer cell activity and glucose control in patients with type 2 diabetes and prediabetes

Impact of Smoking on Human Natural Killer Cell Activity: A Large Cohort Study

Association of neutrophil‐to‐lymphocyte ratio and natural killer cell activity revealed by measurement of interferon‐gamma levels in a healthy population

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