A High-Throughput Microtiter Plate Based Method for the Determination of Peracetic Acid and Hydrogen Peroxide

Abstract

Peracetic acid is gaining usage in numerous industries who have found a myriad of uses for its antimicrobial activity. However, rapid high throughput quantitation methods for peracetic acid and hydrogen peroxide are lacking. Herein, we describe the development of a high-throughput microtiter plate based assay based upon the well known and trusted titration chemical reactions. The adaptation of these titration chemistries to rapid plate based absorbance methods for the sequential determination of hydrogen peroxide specifically and the total amount of peroxides present in solution are described. The results of these methods were compared to those of a standard titration and found to be in good agreement. Additionally, the utility of the developed method is demonstrated through the generation of degradation curves of both peracetic acid and hydrogen peroxide in a mixed solution.

Oxidative decarboxylation of pyruvate by 1-deoxy-d-xyulose 5-phosphate synthase, a central metabolic enzyme in bacteria

“…tive detection of H 2 O 2 (29). Unfortunately, control experiments conducted during this study indicate that H 2 O 2 is unstable under these reaction conditions and, therefore, cannot be accurately detected by this method (Fig.…”

“…To distinguish between paths A and B/C ( Fig. 4), we utilized two previously described biochemical assays that specifically monitor peracetic acid (path A) or H 2 O 2 (paths B or C) formation (20,29). DXP synthase-catalyzed peracetic acid formation was probed using a coupled assay with thionitrobenzoate (TNB, max ϭ 412 nm), which is readily oxidized by peracetic acid at a significantly higher rate (ϳ3000-fold higher) compared with its oxidation by H 2 O 2 (20), thus providing a selective detection method to differentiate between peracetic acid and H 2 O 2 production.…”

AgAu Hollow Nanoshells on Layered Graphene Oxide and Silica Submicrospheres as Plasmonic Nanozymes for Light-Enhanced Electrochemical H2O2 Sensing

“…Hydrogen peroxide (H 2 O 2 ) is an important analyte, and its determination plays a key role in different fields, such as biology, biomedicine, the food industry, and pharmaceutical, biochemical, and environmental analyses. , Moreover, it is involved in several biological processes, and its excess can affect the proper functioning of cells; thus, its accurate determination is valuable . Over the years, different techniques have been explored for its determination, such as titrimetry, chromatography, spectrophotometry, colorimetry, fluorescence, and electrochemistry . However, electrochemical methods are preferred due to their rapid development, good accuracy, lower detection limits, easy miniaturization, biocompatibility, low cost, simple instrumentation, high selectivity, and fast response. − …”

Peracetic Acid Sterilization Induces Divergent Biological Response in Polymeric Tissue Engineering Scaffolds

“…Other studies have reported adsorption of known chemical and gas sterilants (hydrogen peroxide and EtO) onto polymers with some polymer absorbing considerable amounts of H 2 O 2 in less than an hour. [32,35] Studies exist in the literature regarding use of STS and catalase to remove residual concentrations of PA. [36]. Radl et al demonstrated that desorption kinetics from polymers exposed to hydrogen peroxide showed a strong dependence on the material composition and temperature [29].…”