A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control

Abstract

Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC50 values of a representative group of compounds were determined using this high-throughput screening method and compared with LD50 values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.

Biting Deterrence, Repellency, and Larvicidal Activity of <I>Ruta chalepensis</I> (Sapindales: Rutaceae) Essential Oil and Its Major Individual Constituents Against Mosquitoes

“…It is easily accessible to mosquito control districts as it requires very little laboratory equipment and can be used with small numbers of organisms. As we found indications of permethrin resistance in the BEACH strain and wished to relate the bottle bioassay results to an actual dose or concentration, we performed both adult topical and larval bioassays with the same 4 strains using standard methods (Pridgeon et al 2009;Ali et al 2013;Chang et al 2014). These procedures are common in toxicological testing, but are infrequently compared with CDC bottle bioassays, which are mainly used to indicate resistance in field populations.…”

Mosquitocidal Activity of Extracts from &lt;i&gt;Ammi visnaga &lt;/i&gt;(Apiaceae) Seeds

“…The stock solutions were then serially diluted 1:10 in acetone. The assay was performed according to published methods on cold anesthetized female mosquitoes [10]. Relative humidity and temperature were maintained at 80% and 26˚C -27˚C respectively.…”

“…The larvicide assay was performed as described previously with some modification for use with smaller volumes to conserve limited stocks of test compounds [10] [11]. Briefly, eggs for use were hatched in 100 mL of deionized water with approximately 40 mg of ground larval diet (1:1 alfalfa powder: pig chow).…”

Synthesis and larvicidal and adult topical activity of some hydrazidehydrazone derivatives against Aedes aegypti

“…The eggs were hatched and the larvae were maintained at a temperature of 27 ± 2˚C and 60 ± 10 % RH in a photoperiod regimen of 12:12 h (L: D). Bioassays were conducted using the system described by Pridgeon et al [21] to determine the larvicidal activity of these compounds against Ae. aegypti.…”