A highly conserved intraspecies homolog of the Saccharomyces cerevisiae elongation factor-3 encoded by the HEF3 gene

Maurice, Trina C.; Mazzucco, Charles E.; Ramanathan, Chandra S.; Ryan, Brenda; Warr, Glenn A.; Puziss, J W

doi:10.1002/(sici)1097-0061(19980915)14:12<1105::aid-yea313>3.0.co;2-y

Cited by 21 publications

(6 citation statements)

References 21 publications

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“…It was shown to be expressed under zinc-deficiency conditions [30]. Studies from the late 1990s showed a lack of functional complementarity between HEF3 and YEF3 [28,29]. This is consistent with accumulating evidence of the functional divergence of protein paralogs [31][32][33].…”

Section: Introductionsupporting

confidence: 71%

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Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress

Gościńska

Ghahe

Domogała

et al. 2020

Genes

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Translation is a core process of cellular protein homeostasis and, thus, needs to be tightly regulated. The production of newly synthesized proteins adapts to the current needs of the cell, including the response to conditions of oxidative stress. Overall protein synthesis decreases upon oxidative stress. However, the selective production of proteins is initiated to help neutralize stress conditions. In contrast to higher eukaryotes, fungi require three translation elongation factors, eEF1, eEF2, and eEF3, for protein synthesis. eEF1 and eEF2 are evolutionarily conserved, but they alone are insufficient for the translation elongation process. eEF3 is encoded by two paralogous genes, YEF3 and HEF3. However, only YEF3 is essential in yeast, whereas the function of HEF3 remains unknown. To elucidate the cellular function of Hef3p, we used cells that were depleted of HEF3 and treated with H2O2 and analyzed the growth of yeast, global protein production, and protein levels. We found that HEF3 is necessary to withstand oxidative stress conditions, suggesting that Hef3p is involved in the selective production of proteins that are necessary for defense against reactive oxygen species.

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Section: Introductionsupporting

confidence: 71%

“…Knowledge of Hef3p is very limited, and its biological role is unknown. Hef3p is not essential for the mating, sporulation, or vegetative growth of yeast [29]. It was shown to be expressed under zinc-deficiency conditions [30].…”

Section: Introductionmentioning

confidence: 99%

Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress

Gościńska

Ghahe

Domogała

et al. 2020

Genes

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“…The eEF3 antibody was raised against full length eEF3 and could cross react with Hef3, a paralog of eEF3, or with other members of the ABCF protein family such as New1. However, the HEF3 gene is not expressed in vegetative growth (Maurice et al 1998;Sarthy et al 1998) and the New1 protein is approximately 15 kDa larger than eEF3.…”

Section: Western Blottingmentioning

confidence: 99%

Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by integrated stress response regulators Gcn1 and Gcn20

Houston

Platten²,

Connelly³

et al. 2021

RNA

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Ribosome stalls can result in ribosome collisions that elicit quality control responses, one function of which is to prevent ribosome frameshifting, an activity that entails interaction of the conserved yeast protein Mbf1 with uS3 on colliding ribosomes. However, the full spectrum of factors that mediate frameshifting during ribosome collisions is unknown. To delineate such factors in the yeast Saccharomyces cerevisiae, we used genetic selections for mutants that affect frameshifting from a known ribosome stall site, CGA codon repeats. We show that the general translation elongation factor eEF3 and the Integrated Stress Response (ISR) pathway components Gcn1 and Gcn20 modulate frameshifting in opposing manners. We found a mutant form of eEF3 that specifically suppressed frameshifting, but not translation inhibition by CGA codons. Thus, we infer that frameshifting at collided ribosomes requires eEF3, which facilitates tRNA-mRNA translocation and E-site tRNA release in yeast and other single cell organisms. By contrast, we found that removal of either Gcn1 or Gcn20, which bind collided ribosomes with Mbf1, increased frameshifting. Thus, we conclude that frameshifting is suppressed by Gcn1 and Gcn20, although these effects are not mediated primarily through activation of the ISR. Furthermore, we examined the relationship between eEF3-mediated frameshifting and other quality control mechanisms, finding that Mbf1 requires either Hel2 or Gcn1 to suppress frameshifting with wild type eEF3. Thus, these results provide evidence of a direct link between translation elongation and frameshifting at collided ribosomes, as well as evidence that frameshifting is constrained by quality control mechanisms that act on collided ribosomes.

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“…eEF3 related proteins in yeast and other eukaryotes: While most work on eEF3 has focused on the YEF3 product, the form of the protein expressed under laboratory conditions, yeast HEF3 encodes a protein 84% identical to eEF3 (Maurice et al 1998;Sarthy et al 1998). While HEF3 cannot complement the lack of YEF3 when expressed from its own promoter, it can when expressed from the YEF3 promoter.…”

Section: Aa-trna Delivery By Eef1mentioning

confidence: 99%

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

2016

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In this review, we provide an overview of protein synthesis in the yeast Saccharomyces cerevisiae. The mechanism of protein synthesis is well conserved between yeast and other eukaryotes, and molecular genetic studies in budding yeast have provided critical insights into the fundamental process of translation as well as its regulation. The review focuses on the initiation and elongation phases of protein synthesis with descriptions of the roles of translation initiation and elongation factors that assist the ribosome in binding the messenger RNA (mRNA), selecting the start codon, and synthesizing the polypeptide. We also examine mechanisms of translational control highlighting the mRNA cap-binding proteins and the regulation of GCN4 and CPA1 mRNAs.

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A highly conserved intraspecies homolog of the Saccharomyces cerevisiae elongation factor-3 encoded by the HEF3 gene

Cited by 21 publications

References 21 publications

Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress

Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress

Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by integrated stress response regulators Gcn1 and Gcn20

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

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