A highly selective and biocompatible chemosensor for sensitive detection of zinc(<scp>ii</scp>)

Dey, Sudipto; Roy, Anirban; Maiti, Guru Prasad; Mandal, Sushil Kumar; Banerjee, Piyali; Roy, Partha

doi:10.1039/c5nj02723c

Cited by 55 publications

(16 citation statements)

References 51 publications

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“…[1][2][3][4] Metal ions are important for a variety of fundamental biological processes, but can also cause serious environmental and health problems due to their high toxicity.…”

Section: Introductionmentioning

confidence: 99%

A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

Liu

2016

RSC Adv.

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A new fluorescent sensor for selective and sensitive sensing of Cu 2+ and Fe 3+ based on the rational control of multiple mechanisms has been developed. Sensor L, is weak-fluorescent (ϕ=0.029) due to the C=N isomerization and PET process. Upon binding with Cu 2+ , the complex L+Cu 2+ can give a rematkable fluorescence enhancement (ϕ=0.182) with a limit of detection down to 1.3×10 -8 mol/L because of the both inhibition of C=N isomerization and PET process. However, upon binding with Fe 3+ , the original weak fluorescence of L is completely quenched (ϕ=0.002) due to the paramagnetic quenching property. In addition, the lighted complex L+Cu 2+ could be further used as a new platform for ultrasensitive detection of Fe 3+ based on the fluorescence "turn-off" response with a limit of detection down to 2.4×10 -8 mol/L in aqueous solution.

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“…[1][2][3][4] Metal ions are important for a variety of fundamental biological processes, but can also cause serious environmental and health problems due to their high toxicity.…”

Section: Introductionmentioning

confidence: 99%

A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

Liu

2016

RSC Adv.

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“…A low concentration of alginate obtained pore size larger beads and less rigid, which later the entrapped enzyme amylase in the Caalginate gel will be easier to removed out from the matrix, finally lower the amylase activity . Amylase activity immobilized on the concentration of sodium alginate 6 and 7% found to be very low, presumably due to the solution of amylase immobilized has a high viscosity, causing the pore size of beads decreases and then inhibit the transfer of the substrate into the beads amylase immobilized (Dey et al, 2015). The results based on the physical form of beads amylase immobilized on a contact time of 30 minutes produces nonhomogeneous beads which on the surface are stronger and weak bead core.…”

Section: Amylase Immobilization With Various Concentrations Of Sodiummentioning

confidence: 79%

“…immobilized protease with Caalginate support matrix and protease activity obtained residual 35% in the third repetition. (Dey, Maiti, & Roy, 2015) conducted a lipase immobilization by trapping method using alginate matrix and the residual lipase activity of 72% after four times of use. (Baskar, Banu, Leuca, Gayathri, & Jeyashree, 2015) studied amylase immobilization on magnetic nanoparticles and residual activity of 75% after five times of use.…”

Section: Introductionmentioning

confidence: 99%

Immobilization and Characterization of Bacillus Thuringiensis HCB6 Amylase in Calcium Alginate Matrix

Zusfahair

Ningsih

Kartika

et al. 2017

Molekul

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Free enzyme in solution react with substrates to result in products which cannot be recovered for reuse. These problems can be overcome to a certain extent by the use of enzyme immobilization method. Immobilized enzymes are more robust and more resistant to condition changes. More importantly, the heterogeneous immobilized enzyme systems allow an easy recovery of both enzymes and products, multiple re-uses of enzymes, and continuous operation of enzymatic processes. Entrapment of enzymes in Ca-alginate is one of the simplest methods of immobilization. The aim of this research was to obtain the optimum condition of the making of immobilized amylase beads using a Ca-alginate bead and to determine its characteristics. The optimization of immobilized amylase beads includes variation of sodium alginates and variations of enzyme contact time with CaCl2. The characterization of immobilized amylase includes determination of optimum substrate concentration, optimum pH, and optimum incubation time as well as amylase stability test. Amylase activity was determined by using dinitro salicylic (DNS) method. The results showed that the optimum immobilized amylase obtained at alginate concentrations of 5% (w/v), contact time of 60 minutes and immobilization efficiency of 67.5%. Furthermore, immobilized amylase showed optimum substrate concentration of 1.5-2.5% (w/v), optimum pH of 6, an optimum incubation time of 20 minutes with the activity of 179.8 U/mL. The KM value for free amylase and immobilized amylases were 0.3 mM and 0.12 mM respectively. Vmax value for free amylase and immobilized amylases were 105.3 U/mL and 10.1 U/mL respectively. Immobilized Amylase can be used up to six times with the residual activity of 52.7%. Keywords: Amylase, Ca-alginate, enzyme immobilization ABSTRAKEnzim dalam keadaan bebas (larutan) dapat bereaksi dengan substrat menghasilkan produk kemudian tidak dapat digunakan kembali. Permasalahan ini dapat diatasi dengan metode amobilisasi enzim. Enzim amobil lebih kuat dan lebih tahan terhadap perubahan keadaan. Sistem heterogen enzim amobil memungkinkan kemudahan recovery antara enzim dan produk, pemakaian enzim secara berulang, dan proses enzimatik secara kontinu. Penjebakan enzim dalam kalsium alginat (Ca-alginat) adalah salah satu metode amobilisasi paling sederhana. Penelitian ini bertujuan untuk memperoleh kondisi optimum pembuatan beads amilase amobil menggunakan matrik Ca-alginat, dan mengetahui karakteristiknya. Optimasi pembuatan beads amilase amobil meliputi variasi konsentrasi natrium alginat dan variasi waktu kontak enzim dengan CaCl2. Karakterisasi amilase amobil meliputi penentuan konsentrasi substrat optimum, pH optimum, waktu inkubasi optimum serta uji kestabilan amilase. Aktivitas amilase ditentukan dengan menggunakan metode asam dinitrosalisilat (DNS). Hasil penelitian menunjukkan kondisi optimum pembuatan beads amilase amobil diperoleh pada konsentrasi alginat 5 % (b/v) dan waktu kontak 60 menit dengan nilai efisiensi amobilisasi sebesar 67,5 %. Amilase amobil mempunyai ak...

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“…Because the second most plentiful transition metal ion in human body, Zn 2+ plays various critical roles in biological functions including neuronal signal transmission, enzymatic regulations, influencing DNA synthesis, and numerous cellular functions . In this respect, sensitive and noninvasive technique to detect free zinc ions, which are spectroscopically silent due to its 3d 10 electron configurations, is one of the important research areas . However, most of the reported wavelengths of the chemosensors and their emissions are still lies in blue or green region, and only a few molecules showed red or orange emissions, which are better wavelengths in cellular bioimaging …”

Section: Methodsmentioning

confidence: 99%

Thiazole‐based Orange‐emitting Excited‐State Intramolecular Proton Transfer Chemosensors for Selective and Ratiometric Sensing of Zn²⁺ Ions

Kim

Somasundaram

Park

2019

Bulletin Korean Chem Soc

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A highly selective and biocompatible chemosensor for sensitive detection of zinc(ii)

Abstract: A selective fluorescence chemosensor for Zn2+ has been developed with a very low limit of detection value (0.832 nM).

Cited by 55 publications

References 51 publications

A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

Immobilization and Characterization of Bacillus Thuringiensis HCB6 Amylase in Calcium Alginate Matrix

Thiazole‐based Orange‐emitting Excited‐State Intramolecular Proton Transfer Chemosensors for Selective and Ratiometric Sensing of Zn²⁺ Ions

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A highly selective and biocompatible chemosensor for sensitive detection of zinc(ii)

Abstract: A selective fluorescence chemosensor for Zn2+ has been developed with a very low limit of detection value (0.832 nM).

Cited by 55 publications

References 51 publications

A fluorescent sensor for Cu2+and Fe3+based on multiple mechanisms

A fluorescent sensor for Cu2+and Fe3+based on multiple mechanisms

Immobilization and Characterization of Bacillus Thuringiensis HCB6 Amylase in Calcium Alginate Matrix

Thiazole‐based Orange‐emitting Excited‐State Intramolecular Proton Transfer Chemosensors for Selective and Ratiometric Sensing of Zn2+ Ions

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A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

A fluorescent sensor for Cu²⁺and Fe³⁺based on multiple mechanisms

Thiazole‐based Orange‐emitting Excited‐State Intramolecular Proton Transfer Chemosensors for Selective and Ratiometric Sensing of Zn²⁺ Ions