A highly selective and sensitive photoinduced electron transfer (PET) based HOCl fluorescent probe in water and its endogenous imaging in living cells

Liang, Lijuan; Liu, Chang; Jiao, Xiaojie; Zhao, Liancheng; Zeng, Xianshun

doi:10.1039/c6cc02603f

Cited by 99 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…50,53,54 To further investigate the reaction mechanism, we performed mass spectrometry to study the structural variations of SeTBT after reaction with ClO − . The structure of the detection product, oxidized SeTBT, was unambiguously confirmed by MALDI-TOF ([M] + = m/z 549.886) (Figure S9).…”

Section: Resultsmentioning

confidence: 99%

Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid

DuFort³

et al. 2017

J. Am. Chem. Soc.

324

157

View full text Add to dashboard Cite

Developing probes for the detection of reactive oxygen species (ROS), a hallmark of many pathophysiological process, is imperative to both understanding the precise roles of ROS in many life-threatening diseases and optimizing therapeutic interventions. We herein report an all-in-one fluorescent semiconducting polymer based far-red to near-infrared (NIR) Pdot nanoprobe for the ratiometric detection of hypochlorous acid (HOCl). The fabrication takes the advantage of flexible polymer design by incorporating target-sensitive and target-inert fluorophores into a single conjugated polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes. The obtained nanoprobe has improved performance in HOCl sensing, such as high brightness, ideal far-red to NIR optical window, excellent photostability, self-referenced ratiometric response, fast response, and high selectivity. The dual-emission property allows the sensitive imaging of HOCl fluctuations produced in living macrophage cells and peritonitis of living mice with high contrast. This study not only provides a powerful and promising nanoprobe to be potentially used in the investigations of in situ HOCl status of diseases in living systems but also puts forward the design strategy of a new category of ratiometric fluorescent probes facilitating precise and reliable measurement in biological systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid

DuFort³

et al. 2017

J. Am. Chem. Soc.

324

157

View full text Add to dashboard Cite

show abstract

“…From the sigmoidal curve, the apparent dissociation constant (K d ) (7.5053 ± 0.1614) × 10 –5 M (R 2 =0.9959) is obtained. The detection limit was then calculated with the equation: detection limit=3σ bi / m , where σ bi is the standard deviation of blank measurements (σ bi =0.00601, derived from 20 measurements), m is the slope between absorbance at 522 nm versus sample concentration . The detection limit was measured to be 3.9 × 10 –8 M. Thus, 10 aa can function as a potential sensitive optical sensor for ClO – anions.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Stereodefined Multi-Functionalized Tetrasubstituted Olefins via a Catalyst-Free Oxidative Coupling Strategy and Their Application for Hpochlorite Anion Detection

2016

Self Cite

View full text Add to dashboard Cite

We have uncovered an unprecedented catalyst‐free oxidative coupling of (Z)‐2‐ylideneimidazo[1,2‐a]pyridin‐3(2H)‐ones and pyrazolones, which provide a convenient approach to access stereodefined multi‐functionalized tetrasubstituted olefins. These novel stereodefined tetrasubstituted olefins in ethanol exhibited the characteristic intense charge‐transfer π‐π* transitions peaked at ca. 529 nm, and showed excellent selective response to ClO– anion (detection limit: 3.9×10–8 M). This method is notable not only for the ease in which highly functionalized stereodefined tetrasubstituted alkenes can be prepared but also the quite mild catalyst‐free reaction conditions.

show abstract

“…119 exceptional properties like pH-independence, tolerance to photobleaching during excitation laser irradiation, and high HOCl selectivity, which makes it a robust sensor for various biological applications. 120 Li et al 2014, designed first ever probes 73 and 74, Chart 15c as enantioselective fluorescent malate ion sensors for CH 3 CN. The enantioselective sensing properties of these probes was quantified with a 400 MHz NMR spectrometer using probe 73 as a chiral solvating agent.…”

Section: Acridines Based Probes For Biomolecules and Selective Ion Sementioning

confidence: 99%

Medicinal chemistry of acridine and its analogues

Prasher

Sharma

2018

Med. Chem. Commun.

View full text Add to dashboard Cite

show abstract

A highly selective and sensitive photoinduced electron transfer (PET) based HOCl fluorescent probe in water and its endogenous imaging in living cells

Cited by 99 publications

References 41 publications

Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid

Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid

Synthesis of Stereodefined Multi-Functionalized Tetrasubstituted Olefins via a Catalyst-Free Oxidative Coupling Strategy and Their Application for Hpochlorite Anion Detection

Medicinal chemistry of acridine and its analogues

Contact Info

Product

Resources

About