A Highly Selective Fluorescent Probe for the Detection and Imaging of Peroxynitrite in Living Cells

Yang, Dan; Wang, Huali; Sun, Ziqi; Chung, Nga‐Wai; Shen, Jiangang

doi:10.1021/ja0603756

Cited by 273 publications

(144 citation statements)

References 19 publications

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“…To resolve this problem, we developed a novel fluorescent probe, named HKGreen-1, that has highly sensitivity and selectivity for ONOO -. In primary cultured neurons, HKGreen-1 staining fluorescence was highly increased in the SIN-1 treatment group, but no fluorescence was observed in the other RNS-and ROS-treated groups [127] . With this probe, we discovered endogenous ONOO -generation in oxygen-glucose-deprived cortical neurons [128] .…”

Section: Development Of Fluorescent Probes For Peroxynitrite Detectionmentioning

confidence: 82%

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

Chen

et al. 2012

Acta Pharmacol Sin

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109

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Ischemic stroke accounts for nearly 80% of stroke cases. Recanalization with thrombolysis is a currently crucial therapeutic strategy for re-building blood supply, but the thrombolytic therapy often companies with cerebral ischemia-reperfusion injury, which are mediated by free radicals. As an important component of free radicals, reactive nitrogen species (RNS), including nitric oxide (NO) and peroxynitrite (ONOO -), play important roles in the process of cerebral ischemia-reperfusion injury. Ischemia-reperfusion results in the production of nitric oxide (NO) and peroxynitrite (ONOO -) in ischemic brain, which trigger numerous molecular cascades and lead to disruption of the blood brain barrier and exacerbate brain damage. There are few therapeutic strategies available for saving ischemic brains and preventing the subsequent brain damage. Recent evidence suggests that RNS could be a therapeutic target for the treatment of cerebral ischemia-reperfusion injury. Herein, we reviewed the recent progress regarding the roles of RNS in the process of cerebral ischemic-reperfusion injury and discussed the potentials of drug development that target NO and ONOO -to treat ischemic stroke. We conclude that modulation for RNS level could be an important therapeutic strategy for preventing cerebral ischemiareperfusion injury.

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Section: Development Of Fluorescent Probes For Peroxynitrite Detectionmentioning

confidence: 82%

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

Chen

et al. 2012

Acta Pharmacol Sin

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109

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“…For the detection of apoptosis in the heart, we performed the terminal transferase dUTP nick end labeling (TUNEL) assay by using the In Situ Apoptosis Detection Kit (Chemicon International, Temecula, California), and mouse testicular tissue was used as a positive control (16). In addition, immunofluorescent staining was used to localize activated caspase-3 by double stains for cardiomyocytes with alpha sarcomeric actin and caspase-3 (5) and also to directly detect peroxynitrite formation in cultured myocytes with its specific probe HKGreen-1 (kindly provided by Dr. Dan Yang from The University of Hong Kong) (17). Serum and cardiac Ang II were measured with the Ang II Enzyme Immunoassay Kit (SPI-BIO, Massy, France).…”

Section: Methodsmentioning

confidence: 99%

Metallothionein Suppresses Angiotensin II–Induced Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation, Nitrosative Stress, Apoptosis, and Pathological Remodeling in the Diabetic Heart

Zhou

Hein

et al. 2008

Journal of the American College of Cardiology

112

109

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“…Other commonly used fluorescent dyes are based on rhodamine, ethidine and the phenoxazine backbones, well known examples include Amplex Red and Amplex Ultra Red which are based on phenoxazine (resourfin). Commonly used chemiluminescent based indicators include lucigenin as a specific detector for O 2¯• while luminol has mostly been used as an indicator for general ROS production.Based on redox active substituents in the benzene ring and using the nucleophilic character of some reactive species, the development of a new generation of dyes with potentially improved characteristics has been described [31][32][33][34][35][36][37][38][39][40].Because of the quite complex chemistry of all of these dyes, great care has to be taken in designing biological experiments and in their interpretation. For example, there is evidence that DCF may produce secondary radicals induced by its own oxidation [41].…”

Section: Dyesmentioning

confidence: 99%

Redox regulation of calcium ion channels: Chemical and physiological aspects

et al. 2011

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a b s t r a c tReactive oxygen species (ROS) are increasingly recognized as second messengers in many cellular processes. While high concentrations of oxidants damage proteins, lipids and DNA, ultimately resulting in cell death, selective and reversible oxidation of key residues in proteins is a physiological mechanism that can transiently alter their activity and function. Defects in ROS producing enzymes cause disturbed immune response and disease.Changes in the intracellular free Ca 2+ concentration are key triggers for diverse cellular functions. Ca 2+ homeostasis thus needs to be precisely tuned by channels, pumps, transporters and cellular buffering systems. Alterations of these key regulatory proteins by reversible or irreversible oxidation alter the physiological outcome following cell stimulation. It is therefore necessary to understand which proteins are regulated and if this regulation is relevant in a physiological-and/or pathophysiological context. Because ROS are inherently difficult to identify and to measure, we first review basic oxygen redox chemistry and methods of ROS detection with special emphasis on electron paramagnetic resonance (EPR) spectroscopy. We then focus on the present knowledge of redox regulation of Ca 2+ permeable ion channels such as voltage-gated (CaV) Ca 2+ channels, transient receptor potential (TRP) channels and Orai channels.© 2011 Elsevier Ltd. All rights reserved. Basic redox chemistry of oxygenMany chemical processes are linked to the exchange of electrons between two or more molecular entities. The transfer of one (or more) electron(s) is associated with oxidation (loss of electron) and reduction (gain of electron) of the components. Such reactions are also known as redox reactions. The processes of reactive oxygen species (ROS) formation and elimination are exclusively of redox nature.Molecular oxygen is the precursor of all ROS. It contains two unpaired electrons in separate (antibonding) orbitals in its outer electron sphere and is thus, by definition, a radical. Radicals have at least one unpaired electron in their orbital system and usually show high reactivity; transition metal ions also may have unpaired electrons, but are not called radicals. Although having radical character, molecular oxygen is chemically rather inert (luckily!), because high * Corresponding authors at: Institut für Biophysik, Gebäude 58, Universität des Saarlandes, D-66421 Homburg/Saar, Germany. Tel.: +49 6841 1626453; fax: +49 6841 1626060.E-mail addresses: ivan.bogeski@uks.eu (I. Bogeski), barbara.niemeyer@uks.eu (B.A. Niemeyer).

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A Highly Selective Fluorescent Probe for the Detection and Imaging of Peroxynitrite in Living Cells

Cited by 273 publications

References 19 publications

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

Metallothionein Suppresses Angiotensin II–Induced Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation, Nitrosative Stress, Apoptosis, and Pathological Remodeling in the Diabetic Heart

Redox regulation of calcium ion channels: Chemical and physiological aspects

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