A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

Pothipor, Chammari; Aroonyadet, Noppadol; Jakmunee, Jaroon

doi:10.1039/d1an00116g

Cited by 68 publications

(26 citation statements)

References 68 publications

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“…The peak current of the MB redox process, which was proportional to the miRNA-21 concentration on the electrode surface, was monitored via differential pulse voltammetry (DPV). The new biosensor showed a linear range from 1.0 fM–1.0 nM with a LOD of 0.020 fM, with good selectivity, high stability, and satisfactory reproducibility [ 49 ].…”

Section: Outcomementioning

confidence: 99%

Role of Nano-miRNAs in Diagnostics and Therapeutics

Coradduzza

Bellu

Congiargiu

et al. 2022

IJMS

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MicroRNAs (miRNA) are key regulators of gene expression, controlling different biological processes such as cellular development, differentiation, proliferation, metabolism, and apoptosis. The relationships between miRNA expression and the onset and progression of different diseases, such as tumours, cardiovascular and rheumatic diseases, and neurological disorders, are well known. A nanotechnology-based approach could match miRNA delivery and detection to move beyond the proof-of-concept stage. Different kinds of nanotechnologies can have a major impact on the diagnosis and treatment of miRNA-related diseases such as cancer. Developing novel methodologies aimed at clinical practice represents a big challenge for the early diagnosis of specific diseases. Within this context, nanotechnology represents a wide emerging area at the forefront of research over the last two decades, whose potential has yet to be fully attained. Nanomedicine, derived from nanotechnology, can exploit the unique properties of nanometer-sized particles for diagnostic and therapeutic purposes. Through nanomedicine, specific treatment to counteract only cancer-cell proliferation will be improved, while leaving healthy cells intact. In this review, we dissect the properties of different nanocarriers and their roles in the early detection and treatment of cancer.

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Section: Outcomementioning

confidence: 99%

Role of Nano-miRNAs in Diagnostics and Therapeutics

Coradduzza

Bellu

Congiargiu

et al. 2022

IJMS

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“…Therefore, MB is often employed for sensitive electrochemical detection of microRNA due to its signal amplification properties. For instance, an electrochemical sensor modified using graphene, polypyrrole, and gold nanoparticles was applied for the detection of miR-21 based on intercalating MB signal amplification [49]. The peak current of MB redox process was proportional to miR-21 concentration, achieving low detection limit of 0.02 fM.…”

Section: Intercalation Of Redox Mediatormentioning

confidence: 99%

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Low

Chai

et al. 2021

Micromachines

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MicroRNAs (miRNAs) are important non-coding, single-stranded RNAs possessing crucial regulating roles in human body. Therefore, miRNAs have received extensive attention from various disciplines as the aberrant expression of miRNAs are tightly related to different types of diseases. Furthermore, the exceptional stability of miRNAs has presented them as biomarker with high specificity and sensitivity. However, small size, high sequence similarity, low abundance of miRNAs impose difficulty in their detection. Hence, it is of utmost importance to develop accurate and sensitive method for miRNA biosensing. Electrochemical biosensors have been demonstrated as promising solution for miRNA detection as they are highly sensitive, facile, and low-cost with ease of miniaturization. The incorporation of nanomaterials to electrochemical biosensor offers excellent prospects for converting biological recognition events to electronic signal for the development of biosensing platform with desired sensing properties due to their unique properties. This review introduces the signal amplification strategies employed in miRNA electrochemical biosensor and presents the feasibility of different strategies. The recent advances in nanomaterial-based electrochemical biosensor for the detection of miRNA were also discussed and summarized based on different types of miRNAs, opening new approaches in biological analysis and early disease diagnosis. Lastly, the challenges and future prospects are discussed.

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“…For example, Pothipor et al demonstrated an increase in sensing performance by introducing small amounts of polypyrrole in a microRNA electrochemical sensor. [ 13 ] Thin films of polypyrrole have been used as supports for enzymes for their adsorptive properties. [ 14 ] If the load of polypyrrole is relatively high, it can increase the capacitance of the electrode, which can be useful for different electronic devices.…”

Section: Introductionmentioning

confidence: 99%

A facile method for generating polypyrrole microcapsules and their application in electrochemical sensing

et al. 2022

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A facile and rapid strategy to generate polypyrrole microcapsules is reported. The strategy is compatible with a vortex mixer and with a microfluidic chip for droplet generation, allowing a > 100-fold reduction in particle size. The sub-micron particle sizes obtained can also be tuned to some extent based on the chip geometry. The capsules can be kept stably in solution and can be transferred onto electrochemical devices. As an application example, we casted the polypyrrole capsules generated onto screen-printed electrodes, leading to a significant increase in their electroactive surface area and capacitance. The electrodes were further modified with glucose dehydrogenase (GDH) to fabricate glucose biosensors. The introduction of polypyrrole microcapsules increased the dynamic range of the glucose sensor to ca. 300% compared with that of the electrode without polypyrrole microcapsules. The resulting glucose sensor is operated at a constant applied potential of 0.20 V vs. Ag/AgCl (3 M KCl) in an air-equilibrated electrolyte. At this potential, the sensor showed a linear range from 1.0 to 9.0 mM glucose with a sensitivity of 3.23 µA cm−2 mM−1 (R2 = 0.993). The limit of detection obtained was 0.09 mM, and the reproducibility was 3.6%. The method allows generating polypyrrole microcapsules without surfactants or organic solvents and may enable new opportunities in the design of biosensors, electronic devices, and molecular delivery. Graphical abstract

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A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

Abstract: Numerous clinical studies suggest that microRNAs (miRNAs) are indicative biomolecules for the early diagnosis of cancer. This work aims to develop a cost-effective and label-free electrochemical biosensor to detect miRNA-21,...

Cited by 68 publications

References 68 publications

Role of Nano-miRNAs in Diagnostics and Therapeutics

Role of Nano-miRNAs in Diagnostics and Therapeutics

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

A facile method for generating polypyrrole microcapsules and their application in electrochemical sensing

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