A highly sensitive electrochemiluminescence biosensor for the detection of organophosphate pesticides based on cyclodextrin functionalized graphitic carbon nitride and enzyme inhibition

Abstract

A signal on an electrochemiluminescence (ECL) biosensor using β-cyclodextrin (CD) functionalized graphitic carbon nitride (g-C3N4) as the luminophore was constructed for sensitive organophosphate pesticides (OPs) detection based on the enzyme inhibition of OPs, showing that the consumption of coreactant triethylamine (Et3N) decreased with a lessening of the acetic acid (HAc) in situ generated by enzymatic reaction.

Graphitic-phase carbon nitride-based electrochemiluminescence sensing analyses: recent advances and perspectives

“…Aer acetylcholinesterase (AChE) was chemically conjugated onto inclusion complex modied electrode, a sensitive "signal-on" ECL sensor was constructed for organophosphate pesticides (OPs) determination. 79 In the absence of OPs, the hydrolysis reaction of acetylthiocholine (ATCl) by AChE could in situ (Fig. 8).…”

Recent Advances Based on Nanomaterials as Electrochemiluminescence Probes for the Fabrication of Sensors

“…For example, a non‐enzymatic ECL sensor based on the competitive reaction between glucose and phenoxy dextran (DexP) for concanavalin A (ConA) binding sites was constructed for glucose detection . To achieve the versatile analysis, recently, Yuan's group reported an ECL biosensor for organophosphate pesticides (OPs) detection based on acetylcholinesterase (AChE) modified g‐C 3 N 4 as emitter and Et 3 N as co‐reactant.…”

Nanomaterials in Electrochemiluminescence Sensors

“…On this basis, stable and defined G‐C 3 N 4 nanoflake films, which exhibited high ECL sensitivity to co‐reactants such as O 2 , H 2 O 2 and S 2 O 8 2− , were easily formed on hydrophilic substrates. Recently, Yuan and coworkers proposed the β‐cyclodextrin (CD) functionalized G‐C 3 N 4 as another strategy for stretching the bulk structure and obtaining highly dispersed G‐C 3 N 4 nanosheets . An ultrasensitive ECL biosensor for the detection of organophosphate pesticides was achieved based on G‐C 3 N 4 −CD nanocomposites, which showed four times higher ECL intensity than that of the bulk G‐C 3 N 4 .…”