A Highly Sensitive Electrochemiluminescence Choline Biosensor Based on Poly(aniline‐luminol‐hemin) Nanocomposites

Abstract

Poly(aniline‐luminol‐hemin) nanocomposites are prepared on an electrode surface through electropolymerization, and a highly sensitive electrochemiluminescence (ECL) biosensor for choline is developed based on the poly(aniline‐luminol‐hemin) nanocomposites and an enzyme catalyzed reaction of choline oxidase (CHOD). The obtained nanocomposites are characterized by scanning electron microscopy (SEM), atomic absorption spectrometry (AAS) and ECL. The results indicate that hemin can be incorporated into the poly(aniline‐luminol) nanocomposites using the facile electropolymerization method, and the poly(aniline‐luminol‐hemin) nanocomposites are rod shaped porous nanostructure. Moreover, the poly(aniline‐luminol‐hemin) nanocomposites exhibit higher ECL intensity than poly(aniline‐luminol) nanocomposites in alkaline media due to the catalytic effect of hemin on the ECL of the polymerized luminol and the electron transfer ability of hemin in the nanocomposites. CHOD is immobilized on the surface of the poly(aniline‐luminol‐hemin) nanocomposites modified electrode with glutaraldehyde, and the ECL biosensor based on poly(aniline‐luminol‐hemin)/CHOD exhibits a wider linear range for the choline detection. The enhanced ECL signals are linear with the logarithm of concentration of choline over the range of 1.0×10−11∼1.0×10−7 mol L−1 with a low detection limit of 1.2×10−12 mol L−1. Moreover, the proposed biosensor is successfully applied to the detection of choline in milk.

Electrochemiluminescence DNA Biosensor for HBV Based on Coralloid Poly(Aniline‐Luminol)‐MWCNTs and Catalysis of Ferrocene

“…Synthesis of P(ANiÀ Lu) on MWCNTs was as follows: the graphite electrode modified by MWCNTs was placed in a mixed solution containing 0.025 M aniline sulphate, 3.0 mM luminol and 0.05 M H 2 SO 4 , and the P(ANiÀ Lu) was polymerized according to the reference [17]. Different from the reference, the number of cycling circles was optimized to 20 to better exert the excellent characteristics of MWCNTs.…”

“…The P(ANiÀ Lu)-MWCNTs nanocomposites were also characterized by UV-vis spectra. The pre-processing of the P(ANiÀ Lu)-MWCNTs nanocomposites was carried out according to the literature [17]. The desquamated P(ANiÀ Lu)-MWCNTs was dispersed in ultrapure water.…”

Exploiting poly(safranine) and poly(luminol) for sensing applications. A mini review

“…10 Recently, the uses of luminol and its small molecule derivatives have been widely employed to develop sensing properties toward materials as both electrochemical and biosensors. 7,[47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] Over the past few years, water-soluble dyes, such as carmine, methylene dye derivatives, and other dyes, have been broadly employed in catalyzing oxidation or reducing organic molecules as mediators. [11][12][13] Safranine [Fig.…”

Highly Sensitive and Selective Detection of Pb(II) by NH2−SiO2/Ru(bpy)32+−UiO66 based Solid‐state ECL Sensor

“…These properties limit their wide applications. Electrochemiluminescence (ECL) is considered as a promising and powerful analytical technique because of its unique advantages [16,17]: ECL integrates the advantages of high sensitivity of luminescence analysis and controllable potential of electrochemical analysis, which shows the characteristics of good reproducibility, high sensitivity and good selectivity [18,19]. Zhu et al firstly applied Ru(bpy) 3 2 + derivative as ECL signal molecule and labeled it on DNA by coupling agent to obtain a DNA off-based signal off type lead ion sensor, this sensor could detect lead ions up to 1.1 × 10 À 5 μM [20]; Hai et al modified an aptamer probe with a hairpin structure to the surface of Fe 3 O 4 À Au magnetic nanoparticles, and formed a G-quadruplex with lead ions to construct a signal on-type Pb(II) biosensor and showed high selectivity for Pb(II) with a detection limit of 1.1 × 10 À 5 μM [21].…”

“…These properties limit their wide applications. Electrochemiluminescence (ECL) is considered as a promising and powerful analytical technique because of its unique advantages : ECL integrates the advantages of high sensitivity of luminescence analysis and controllable potential of electrochemical analysis, which shows the characteristics of good reproducibility, high sensitivity and good selectivity . Zhu et al.…”