A highly sensitive magnetite nanoparticle as a simple and rapid stem cell labelling agent for MRI tracking

Kim, Hyun‐Jin; Dae, Hyun-Mi; Park, Cheong-Soo; Kim, Eun Ock; Kim, Daehong; Kim, In-Hoo; Kim, Yun-Hee; Choi, Yongdoo

doi:10.1039/c1jm10247h

Cited by 19 publications

(19 citation statements)

References 24 publications

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“…The relaxivity values, determined by fitting these data and considering the Fe concentration in Sor‐Mag‐SLNs determined by elemental analysis, and their ratio ( r 2 / r 1 ) are reported in Table 1 together with relaxivities of commercial contrast agents based on SPIONs. These results indicate that Sor‐Mag‐SLNs have good negative contrast properties at the investigated fields, in particular showing r 2 (38 ± 2 s −1 m m −1 at 7.05 T, 215 ± 5 s −1 m m −1 at 0.47 T) and r 2 / r 1 (633 at 7.05 T, 77 at 0.47 T) values much higher than commercial agents at magnetic fields of clinical interest …”

Section: Resultsmentioning

confidence: 67%

Active Targeting of Sorafenib: Preparation, Characterization, and In Vitro Testing of Drug‐Loaded Magnetic Solid Lipid Nanoparticles

Grillone

Riva

Mondini

et al. 2015

Adv Healthcare Materials

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Sorafenib is an anticancer drug approved by the Food and Drug Administration for the treatment of hepatocellular and advanced renal carcinoma. The clinical application of sorafenib is promising, yet limited by its severe toxic side effects. The aim of this study is to develop sorafenib-loaded magnetic nanovectors able to enhance the drug delivery to the disease site with the help of a remote magnetic field, thus enabling cancer treatment while limiting negative effects on healthy tissues. Sorafenib and superparamagnetic iron oxide nanoparticles are encapsulated in solid lipid nanoparticles by a hot homogenization technique using cetyl palmitate as lipid matrix. The obtained nanoparticles (Sor-Mag-SLNs) have a sorafenib loading efficiency of about 90% and are found to be very stable in an aqueous environment. Plain Mag-SLNs exhibit good cytocompatibility, whereas an antiproliferative effect against tumor cells (human hepatocarcinoma HepG2) is observed for drug-loaded Sor-Mag-SLNs. The obtained results show that it is possible to prepare stable Sor-Mag-SLNs able to inhibit cancer cell proliferation through the sorafenib cytotoxic action, and to enhance/localize this effect in a desired area thanks to a magnetically driven accumulation of the drug. Moreover, the relaxivity properties observed in water suspensions hold promise for Sor-Mag-SLN tracking through clinical magnetic resonance imaging.

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Section: Resultsmentioning

confidence: 67%

Active Targeting of Sorafenib: Preparation, Characterization, and In Vitro Testing of Drug‐Loaded Magnetic Solid Lipid Nanoparticles

Grillone

Riva

Mondini

et al. 2015

Adv Healthcare Materials

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“…Then Fe 3 O 4 /PAA composite NPs could be easily obtained by hydrolysis. Kim et al 71 prepared PAA coated SPIONs by exchanging the oleic acid on the surface of SPIONs with PAA. Subsequently, the free carboxylic acids of the PAA backbone were conjugated with 2-aminoethyl-trimethyl ammonium (TMA) by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) initiation to produce particles called as ''TMA-SPION''.…”

Section: Polymersmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking

Li¹,

Jiang²,

Luo³

et al. 2013

Theranostics

433

307

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Stem cells hold great promise for the treatment of multiple human diseases and disorders. Tracking and monitoring of stem cells in vivo after transplantation can supply important information for determining the efficacy of stem cell therapy. Magnetic resonance imaging (MRI) combined with contrast agents is believed to be the most effective and safest non-invasive technique for stem cell tracking in living bodies. Commercial superparamagnetic iron oxide nanoparticles (SPIONs) in the aid of transfection agents (TAs) have been applied to labeling stem cells. However, owing to the potential toxicity of TAs, more attentions have been paid to develop novel SPIONs with specific surface coating or functional moieties which facilitate effective cell internalization in the absence of TAs. This review aims to summarize the recent progress in the design and preparation of SPIONs as cellular MRI probes, to discuss their applications and current problems facing in stem cell labeling and tracking, and to offer perspectives and solutions for the future development of SPIONs in this field.

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“…This approach, although is a powerful tool in animal studies, has limited value in terms of clinical translation 7, 9, 10. On the other hand, several different methods to label stem cells with exogenous probes including quantum dots (QDs),11, 12 magnetic nanopartciles,13–15 carbon nanotubes,16 and silicon nanoparticles17 have also been developed for in vivo stem cell tracking via various imaging modalities such as optical imaging, magnetic resonance imaging (MRI), and radionucleotide based imaging 15, 18, 19. However, due to the intrinsic limits of current imaging techniques and tracking probes, the accurate detection of small numbers of cells, as well as the remote control of stem cells translocation after in vivo transplantation, remains challenging in stem cell research.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Upconversion Nanoparticles for Dual‐Modal Imaging‐Guided Stem Cell Therapy under Remote Magnetic Control

Cheng

Wang

et al. 2012

Adv Funct Materials

144

100

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Stem cells have generated a great deal of excitement in cell‐based therapies. Here, a unique class of multifunctional nanoparticles (MFNPs) with both upconversion luminescence (UCL) and superparamagnetic properties is used for stem cell research. It is discovered that after being labeled with MFNPs, mouse mesenchymal stem cells (mMSCs) are able to maintain their viability and differentiation ability. In vivo UCL imaging of MFNP‐labeled mMSCs transplanted into animals is carried out, achieving ultrahigh tracking sensitivity with a detection limit as low as ≈10 cells in a mouse. Using both UCL optical and magnetic resonance (MR) imaging approaches, MFNP‐labeled mMSCs are tracked after being intraperitoneally injected into wound‐bearing mice under a magnetic field. The translocation of mMSCs from the injection site to the wound nearby the magnet is observed and, intriguingly, a remarkably improved tissue repair effect is observed as the result of magnetically induced accumulation of stem cells in the wound site. The results demonstrate the use MFNPs as novel multifunctional probes for labeling, in vivo tracking, and manipulation of stem cells, which is promising for imaging guided cell therapies and tissue engineering.

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A highly sensitive magnetite nanoparticle as a simple and rapid stem cell labelling agent for MRI tracking

Cited by 19 publications

References 24 publications

Active Targeting of Sorafenib: Preparation, Characterization, and In Vitro Testing of Drug‐Loaded Magnetic Solid Lipid Nanoparticles

Active Targeting of Sorafenib: Preparation, Characterization, and In Vitro Testing of Drug‐Loaded Magnetic Solid Lipid Nanoparticles

Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking

Multifunctional Upconversion Nanoparticles for Dual‐Modal Imaging‐Guided Stem Cell Therapy under Remote Magnetic Control

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