A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

Fu, Manlin; Shen, Wei; Chen, Yajun; Yi, Wenjun; Cai, Chunhui; Zhu, Liquan; Zhu, Qing

doi:10.1039/c9tb02023c

Cited by 44 publications

(23 citation statements)

References 45 publications

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“…The chemical structure of probe 1 was clearly identified using 1 H and 13 C NMR as well as HR-ESI-MS spectroscopy (Figures S1−S3).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In this context, a fluorescent probe that is capable of simultaneously detecting viscosity and NO in living cells is conceivable; such a probe could provide essential information for understanding cellular oxidative stress and related physiological processes. To date, detection of intracellular viscosity , or NO , in living cells has been performed using fluorescent chemical probes. Recently, the development of dual-responsive fluorescent probes capable of simultaneously detecting multiple analytes is being emphasized in biology. , For example, a new type of probe has emerged, which is capable of concomitant detection of viscosity and reactive redox species, such as H 2 O 2 , ONOO – , • OH, or H 2 S in cells. − However, a fluorescent probe capable of dual detection of viscosity and NO has never been attempted, despite strong anticipation of a significant correlation between viscosity and NO production in cells.…”

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A Fluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric Oxide in Living Cells

et al. 2021

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Intracellular viscosity is a physicochemical factor that determines the outcomes of various biological processes, while nitric oxide (NO) is an essential signaling molecule that controls many cellular processes, including oxidative stress. Anticipating that both may be interrelated with a variety of pathologies, their simultaneous measurement would be highly valuable for the investigation of the pathological condition of cells. However, the development of a sensor for such simultaneous detection has not been attempted yet. Herein, we present the synthesis of naphthalimide-4-(4-nitrophenyl)thiosemicarbazide, probe 1, and its application to living cells under conditions of lipopolysaccharide or nystatin treatment, adopted as oxidative stress and altered intracellular viscosity models, respectively. The probe showed increased fluorescence in response to elevation of viscosity and NO levels at 470 and 550 nm, respectively, in the solution studies. When the probe was used for a confocal microscopic study of HeLa cells under stressed conditions, simultaneous monitoring of viscosity and NO level elevations was possible through fluorescence imaging using band-pass filters of 420−475 and 505−600 nm, respectively, upon excitation at a wavelength of 405 nm. Interestingly, both the cellular viscosity and NO levels increased together under lipopolysaccharide or nystatin treatment. Therefore, we suggest that probe 1 is a fluorescent chemical probe that enables the monitoring of alterations in intracellular viscosity and NO levels in living cells, which would be valuable in studies of various cellular damage models.

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“…The chemical structure of probe 1 was clearly identified using 1 H and 13 C NMR as well as HR-ESI-MS spectroscopy (Figures S1−S3).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A Fluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric Oxide in Living Cells

et al. 2021

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“…The Zhu group developed a red‐emitting fluorescent probe 56 to detect viscosity [85] . This probe was composed of dicyanoisophorone and modified julolidine (Figure 25).…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

118

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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“…23 Subsequently, it is reported that AIE molecules exhibit a special response to the viscosity of solvents. 24,25 More importantly, some AIE molecules are also sensitive to the polarity of solvents. This greatly stimulates our interest in monitoring the viscosity and polarity of lubricants using AIE molecules.…”

Section: Introductionmentioning

confidence: 99%

A triphenylamine-based aggregation-enhanced emission probe for viscosity and polarity analysis of lubricating oils

et al. 2022

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A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

Abstract: Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo.

Cited by 44 publications

References 45 publications

Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A Fluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric Oxide in Living Cells

Naphthalimide-4-(4-nitrophenyl)thiosemicarbazide: A Fluorescent Probe for Simultaneous Monitoring of Viscosity and Nitric Oxide in Living Cells

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

A triphenylamine-based aggregation-enhanced emission probe for viscosity and polarity analysis of lubricating oils

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