A highly-sensitive rice seedling bioassay for the detection of femtomole quantities of 3?-hydroxylated gibberellins

Nishijima, Takaaki; Koshioka, Masaji; Yamazaki, Hiroko

doi:10.1007/bf00024844

Cited by 10 publications

(9 citation statements)

References 6 publications

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“…Figure 2 shows the effects of various GAs (10 À5 M) on the GID1-SLR1 interaction in yeast cells (Figure 2A) and the dose dependency of second leaf sheath elongation in seedlings of a GA-deficient rice mutant, Tan-Ginbozu, after a one-drop treatment of various GAs ( Figure 2B). The one-drop treatment of GAs on Tan-Ginbozu seedlings is a classic bioassay for estimation of GA levels because Tan-Ginbozu seedlings sensitively and quantitatively respond to the exogenously added GA (Nishijima et al, 1993). The trend of effectiveness for various GAs in the GID1-SLR1 interaction in yeast and for leaf sheath elongation of Tan-Ginbozu was almost identical except for GA 3 and GA 9 .…”

Section: Resultsmentioning

confidence: 99%

Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin

et al. 2007

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GIBBERELLIN INSENSITIVE DWARF1 (GID1) encodes a soluble gibberellin (GA) receptor that shares sequence similarity with a hormone-sensitive lipase (HSL). Previously, a yeast two-hybrid (Y2H) assay revealed that the GID1-GA complex directly interacts with SLENDER RICE1 (SLR1), a DELLA repressor protein in GA signaling. Here, we demonstrated, by pulldown and bimolecular fluorescence complementation (BiFC) experiments, that the GA-dependent GID1-SLR1 interaction also occurs in planta. GA 4 was found to have the highest affinity to GID1 in Y2H assays and is the most effective form of GA in planta. Domain analyses of SLR1 using Y2H, gel filtration, and BiFC methods revealed that the DELLA and TVHYNP domains of SLR1 are required for the GID1-SLR1 interaction. To identify the important regions of GID1 for GA and SLR1 interactions, we used many different mutant versions of GID1, such as the spontaneous mutant GID1s, N-and C-terminal truncated GID1s, and mutagenized GID1 proteins with conserved amino acids replaced with Ala. The amino acid residues important for SLR1 interaction completely overlapped the residues required for GA binding that were scattered throughout the GID1 molecule. When we plotted these residues on the GID1 structure predicted by analogy with HSL tertiary structure, many residues were located at regions corresponding to the substrate binding pocket and lid. Furthermore, the GA-GID1 interaction was stabilized by SLR1. Based on these observations, we proposed a molecular model for interaction between GA, GID1, and SLR1.

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Section: Resultsmentioning

confidence: 99%

Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin

et al. 2007

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“…4 ). GA 1 is known as the main growth-active GA, while the other GAs are not active until they are converted to GA 1 19 34 35 . Studies of maize have shown that tall plants have a higher GA 1 level, while GA 1 is absent or present at a very low level in dwarf plants 36 .…”

Section: Discussionmentioning

confidence: 99%

Involvement of Auxin and Brassinosteroid in Dwarfism of Autotetraploid Apple (Malus × domestica)

Hou

Zhang

et al. 2016

Sci Rep

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The plant height is an important trait in fruit tree. However, the molecular mechanism on dwarfism is still poorly understood. We found that colchicine-induced autotetraploid apple plants (Malus × domestica) exhibited a dwarf phenotype. The vertical length of cortical parenchyma cells was shorter in autotetraploids than in diploids, by observing paraffin sections. Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR) were significantly decreased in 3- and 5-year-old autotetraploid plants. Digital gene expression (DGE) analysis showed that the differentially expressed genes were mainly involved in IAA and BR pathways. microRNA390 was significantly upregulated according to microarray analysis. Exogenous application of IAA and BR promoted stem elongation of both apple plants grown in medium. The results show that dwarfing in autotetraploid apple plants is most likely regulated by IAA and BR. The dwarf phenotype of autotetraploid apple plants could be due to accumulation of miR390 after genome doubling, leading to upregulation of apple trans-acting short-interfering RNA 3 (MdTAS3) expression, which in turn downregulates the expression of MdARF3. Overall, this leads to partial interruption of the IAA and BR signal transduction pathway. Our study provides important insights into the molecular mechanisms underlying dwarfism in autopolyploid apple plants.

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“…4 ) Neither PBZ nor ACD caused deterioration (e.g., non-uniform germination or growth inhibition) of the seedlings when used simultaneously with PHC. Those combinations of the chemicals made the three rice cultivars almost as sensitive and specific to 3fJ-hydroxylated GAs (GAl' GA 3 , GA 4 , and GA 7 ) as PHC plus UCZ did (Table II).…”

mentioning

confidence: 93%

Use of Several Gibberellin Biosynthesis Inhibitors in Sensitized Rice Seedling Bioassays

Nishijima¹,

Koshioka²,

Yamazaki³

1994

Bioscience, Biotechnology, and Biochemistry

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A highly-sensitive rice seedling bioassay for the detection of femtomole quantities of 3?-hydroxylated gibberellins

Cited by 10 publications

References 6 publications

Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin

Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin

Involvement of Auxin and Brassinosteroid in Dwarfism of Autotetraploid Apple (Malus × domestica)

Use of Several Gibberellin Biosynthesis Inhibitors in Sensitized Rice Seedling Bioassays

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