A highly sensitive ultra-high performance liquid chromatography/tandem mass spectrometry method with in-source fragmentation for rapid quantification of raspberry ketone

Yuan, Bo; Zhao, Danyue; Du, Ruoyuan; Kshatriya, Dushyant; Bello, Nicholas T.; Simon, James E.; Wu, Qingli

doi:10.1016/j.jfda.2018.07.005

Cited by 18 publications

(9 citation statements)

References 16 publications

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“…Half of the HFD fed mice ( n = 12) were orally gavaged with acarbose (Acar, 5 mg/kg; cat# A0802; ≥ 98%; LKT Labs, St. Paul, MN, United States), and the other half were orally gavaged with raspberry ketone [RK, 200 mg/kg; 4(4-hydroxyphenyl)-2-butanone; 99%; cat# 178519; Lot# MKBQ9010V; Sigma Aldrich, St. Louis, MO, United States]. A reference sample of RK from the lot number was deposited in a secure, climate-controlled repository ( Yuan et al, 2019 ). The LFD-fed mice were given vehicle (Veh; 50% propylene glycol, 40% water, and 10% dimethyl sulfoxide (DMSO) as a vehicle control immediately after the baseline blood glucose test.…”

Section: Methodsmentioning

confidence: 99%

Reduced Body Fat and Epididymal Adipose Apelin Expression Associated With Raspberry Ketone [4-(4-Hydroxyphenyl)-2-Butanone] Weight Gain Prevention in High-Fat-Diet Fed Mice

Hao

Bello

2021

Front. Physiol.

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Raspberry ketone [4-(4-hydroxyphenyl)-2-butanone] is a natural aromatic compound found in raspberries and other fruits. Raspberry ketone (RK) is synthetically produced for use as a commercial flavoring agent. In the United States and other markets, it is sold as a dietary supplement for weight control. The potential of RK to reduce or prevent excessive weight gain is unclear and could be a convergence of several different actions. This study sought to determine whether acute RK can immediately delay carbohydrate hyperglycemia and reduce gastrointestinal emptying. In addition, we explored the metabolic signature of chronic RK to prevent or remedy the metabolic effects of diet-induced weight gain. In high-fat diet (HFD; 45% fat)-fed male mice, acute oral gavage of RK (200 mg/kg) reduced hyperglycemia from oral sucrose load (4 g/kg) at 15 min. In HFD-fed female mice, acute oral RK resulted in an increase in blood glucose at 30 min. Chronic daily oral gavage of RK (200 mg/kg) commencing with HFD access (HFD_RK) for 11 weeks resulted in less body weight gain and reduced fat mass compared with vehicle treated (HFD_Veh) and chronic RK starting 4 weeks after HFD access (HFD_RKw4) groups. Compared with a control group fed a low-fat diet (LFD; 10% fat) and dosed with vehicle (LFD_Veh), glucose AUC of an oral glucose tolerance test was increased with HFD_Veh, but not in HFD_RK or HFD_RKw4. Apelin (Apln) gene expression in epididymal white adipose tissue was increased in HFD_Veh, but reduced to LFD_Veh levels in the HFD_RK group. Peroxisome proliferator activated receptor alpha (Ppara) gene expression was increased in the hepatic tissue of HFD_RK and HFD_RKw4 groups. Overall, our findings suggest that long term daily use of RK prevents diet-induced weight gain, normalizes high-fat diet-induced adipose Apln, and increases hepatic Ppara expression.

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Section: Methodsmentioning

confidence: 99%

Reduced Body Fat and Epididymal Adipose Apelin Expression Associated With Raspberry Ketone [4-(4-Hydroxyphenyl)-2-Butanone] Weight Gain Prevention in High-Fat-Diet Fed Mice

Hao

Bello

2021

Front. Physiol.

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“…Interestingly, the temperature of the sheath gas rather than that of the drying gas was found to be a more critical factor to “overcook” ions for fragmentation. Suggested by the less curly contours, the sheath gas and drying gas did not present as much an interaction or complementary effect as observed in a prior study 17 . Based on the CCD results, the drying gas temperature was set at 250°C and sheath gas temperature at 300°C.…”

Section: Resultsmentioning

confidence: 91%

“…In this type of experiment, the “source” of a mass spectrometer typically refers to the electrospray ionization (ESI) chamber, the transmission capillary and other spaces maintained under intermediate vacuum by the rough pump prior to the internal high vacuum. While ESI is generally considered as a “soft” ionization technique, ions with weak bonds can still undergo fragmentation during ionization and desorption from the aerosol in the ESI chamber, with the fragmentation intensity affected by the mobile phase and the ESI gas and voltage settings 16–18 . At the end of the transmission capillary, a fragmentor voltage is applied to propel ion transmission, and additional fragmentation could happen due to collision between accelerated ions and the surrounding neutral gas (this is also known as the in‐source collision‐induced dissociation (CID), which is distinguished from the CID occurring in the designated collision cell by collision with an auxiliary gas such as nitrogen, helium or argon) 19,20 .…”

Section: Resultsmentioning

confidence: 99%

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

Lyu

Yuan

Dinssa³

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale The safe consumption of Solanum scabrum and S. nigrum berries (SNBs) depends on a reliable and rapid chemical screen for the testing of the fruit and/or final food and industrial products for the presence and level of toxic glycoalkaloids. Such a rapid and sensitive screen could also be used by those involved in food safety and forensics, industry, research labs and those in agriculture production, breeding and food processing. Significant variation in the content and composition of glycoalkaloids across SNBs has been reported. To facilitate high‐throughput targeted analysis, this work overcame the slow scan speed of a traditional triple quadruple mass spectrometry (QqQ) method by development of a pseudo‐MS3 method. Methods In‐source fragmentation functioned as a pseudo‐MS or pseudo‐hydrolysis to trim down the structurally diverse and complex glycosides into five types of aglycone ions, which were then analyzed using multiple reaction monitoring (MRM). Characteristic product ions were selected based on the aglycone skeleton and substitution pattern and associated fragmentation pathway. Results A compact method with only 15 MRM transitions were developed for high‐throughput screening of very diverse glycoalkaloids. Glycosides of the same aglycone type were readily identified in the same transition window without the need for mass spectra interpretation. Validated using solamargine, the sole available standard, the accuracy was 99.7–101.3%, the intra‐ and inter‐day precision were, respectively, 2.5–5.0% and 8.0–9.2%, and the lower limit of detection and quantification were, respectively, 3.1 and 10.2 ng/mL (with 1 μL injection volume). Conclusions The peudo‐MS3 method allowed for high‐throughput targeted analysis with compact MRM transitions to address a large number of glycoalkaloids with diverse structures. This method could serve to meet the most heavy‐duty demand for rapid inspection of glycoalkaloids in SNBs. This method can be adopted and used by those involved in food safety and forensics, in developing food and industrial products and in genetics and breeding.

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“…The injection volume was 10 μL. Mass spectrometric analysis was performed in both positive and negative ionization modes with a scan rate of 2 spectra/s, mass range of 60–1200 ( m / z ), capillary voltage of 3500 V, and fragmentor setting of 120 V. The pressure of the nebulizer was 40 psi, the gas temperature was 350 °C, and the continuous gas flow was 8 L/min [30] , [31] , [32] .…”

Section: Methodsmentioning

confidence: 99%

Multiblock metabolomics: An approach to elucidate whole-body metabolism with multiblock principal component analysis

Tanabe¹,

Hayashi²,

Katahira³

et al. 2021

Computational and Structural Biotechnology Journal

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A highly sensitive ultra-high performance liquid chromatography/tandem mass spectrometry method with in-source fragmentation for rapid quantification of raspberry ketone

Cited by 18 publications

References 16 publications

Reduced Body Fat and Epididymal Adipose Apelin Expression Associated With Raspberry Ketone [4-(4-Hydroxyphenyl)-2-Butanone] Weight Gain Prevention in High-Fat-Diet Fed Mice

Reduced Body Fat and Epididymal Adipose Apelin Expression Associated With Raspberry Ketone [4-(4-Hydroxyphenyl)-2-Butanone] Weight Gain Prevention in High-Fat-Diet Fed Mice

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

Multiblock metabolomics: An approach to elucidate whole-body metabolism with multiblock principal component analysis

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