A histochemical ATPase method for the demonstration of the muscle capillary network.

Fouces, V.; Torrella, Joan Ramón; Palomeque, J.; Viscor, Ginés

doi:10.1177/41.2.7678272

Cited by 22 publications

(13 citation statements)

References 26 publications

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“…The sections were mounted on gelatinized slides and incubated for 5 min in a buVered Wxative (Viscor et al 1992) in order to prevent shrinkage or wrinkling. After rinsing the slides thoroughly, the following histochemical assays were performed: (1) succinate dehydrogenase, SDH (Nachlas et al 1957), to identify the aerobic and anaerobic Wbres; (2) myoWbrillar adenosine triphosphatase, mATPase (Brooke and Kaiser 1970), following pre-incubation in alkaline (pH 10.7) and acid (pH 4.2 and 4.5) solutions, to diVerentiate between slow and fast Wbres; and (3) the ATPase method developed by Fouces et al (1993), in order to reveal muscle capillaries.…”

Section: Muscles and Histochemical Proceduresmentioning

confidence: 99%

Capillary supply, fibre types and fibre morphometry in rat tibialis anterior and diaphragm muscles after intermittent exposure to hypobaric hypoxia

et al. 2008

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Three groups of sedentary male rats were exposed to intermittent hypobaric hypoxia (IHH) for 22 days (4 h/day, 5 days/week) in a hypobaric chamber at a simulated altitude of 5,000 m. Tibialis anterior (TA) and diaphragm (DG) were removed at the end of the programme (H group), and 20 or 40 days later (P20 and P40 groups). A control group (C) was maintained at sea-level pressure and their TA and DG were compared to those of the experimental rats at the end of the IHH programme, and also 20 and 40 days later. We measured the fibre morphometry and capillaries of each muscle. Our results demonstrate that IHH does not change the fibre type composition (with reference to either their contractile or oxidative properties) for most muscle regions of the muscles analysed analysed. We found few significant differences in muscle capillarity and fibre morphometry for TA after IHH. However, IHH did induce some statistically significant changes in DG: capillary density of the H rats (736 capillaries/mm2) increased compared to C animals (610 capillaries/mm2). Although IHH did not change the fibre capillarization or morphometric parameters of fast fibre types, we observed reductions ranging from 7 to 13% in fibre area, perimeter and diffusion distances between C and H for slow fibres. Moreover, these morphometric changes accounted for increases of 10-20% in capillarization, fibre unit area and fibre unit perimeter. This indicates that SO fibres are more sensitive to IHH than both fast fibre types.

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Section: Muscles and Histochemical Proceduresmentioning

confidence: 99%

Capillary supply, fibre types and fibre morphometry in rat tibialis anterior and diaphragm muscles after intermittent exposure to hypobaric hypoxia

et al. 2008

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“…Sections were mounted on gelatinized slides and incubated for 5 min in a buffered fixative (Viscor et al, 1992) to prevent shrinkage or wrinkling. After rinsing the slides thoroughly, we used the ATPase method developed by Fouces et al (1993) to reveal muscle capillaries.…”

Section: Sampling and Histochemical Proceduresmentioning

confidence: 99%

Capillary Supply and Fiber Morphometry in Rat Myocardium after Intermittent Exposure to Hypobaric Hypoxia

Panisello

Torrella

Pagés

et al. 2007

High Altitude Medicine & Biology

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Panisello, Pere, Joan Ramon Torrella, Teresa Pagés, and Ginés Viscor. Capillary supply and fiber morphometry in rat myocardium after intermittent exposure to hypobaric hypoxia. High Alt. Med. Biol. 8:322-330, 2007.-Three groups of male rats were submitted to an intermittent hypobaric hypoxia (IHH) program for 22 days (4 h/day, 5 days/week) in a hypobaric chamber at a simulated altitude of 5000 m. Hearts were removed at the end of the program (H group) and 20 and 40 days later (P20 and P40 groups). A control group (C) was maintained at sea-level pressure. Transverse sections from myocardium were cut and histochemically stained in order to measure fiber morphometry and capillaries. We observed a progressive increase from C to H to P20 animals in capillary (4124 to 4733 to 4816 capillaries/mm 2 ) and fiber densities (2844 to 3125 to 3284 fibers/mm 2 ) associated with significant reductions in fiber area (273, 235, and 227 m 2 ), perimeter (69, 64, and 62 m), and diffusion distances (18.2, 16.9, and 16.6 m). The most significant differences between C and hypoxic groups were found when morphometrical and vascular fiber parameters were combined. The myocardium of the latter had more capillaries per fiber area and per fiber perimeter. These findings indicate that the IHH program elicits an adaptive response of rat myocardium to a more efficient O2 delivery to mitochondria of cardiac muscle cells. Capillarization and fiber morphometric changes showed marked differences over time. In all cases, P20 had higher capillarization parameters and fiber morphometry reductions than H, thus indicating that a delay of about 20 days exists after the hypoxic stimulus ceases to reach complete angiogenesis and fiber morphometry changes. However, P40 animals showed a recovery to basal values of the parameters related to fiber morphometry (area, perimeter, and diffusion distances), but maintained high capillarity values (capillary density, NCF, CCA, CCP).

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“…Ten microphotographs were taken of each zone in all the muscles subjected to the study, on which the areas were measured, by means of a digitized card (Genitizer-GT-1212B) connected to the Sigma Scan computer program (17). The Sigma Scan program was calibrated with an area equivalent to 10 6 nm 2 .…”

Section: Electron Microscopymentioning

confidence: 99%

Effects of a Standardized Panax ginseng Extract on the Skeletal Muscle of the Rat: A Comparative Study in Animals at Rest and Under Exercise

Ferrando¹,

Vila²,

Voces³

et al. 1999

Planta med

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The effect of standardized Panax ginseng extract G115 on enzymatic activities, myotypological composition, capillaries and mitochondrial content was studied in the skeletal muscle of male rats Wistar. Simultaneously to the G115 administration the rats performed exercise. The animals were divided into 4 groups. The dose of the ginseng extract G115 was 50 mg/kg. The length of the experimental period was 12 weeks. After 24 hours of inactivity the muscles of the hindlimb were extracted. With regard to the enzymatic activities of the citrate synthase (CS) and lactate dehydrogenase (LDH), CS increases with exercise, while the LDH undergoes no major variations, either due to the exercise or the treatment. Treatment with G115 increases the capillary density and the mitochondrial content of the red gastrocnemius muscle. The results suggest that prolonged treatment with G115 increases the capillary density and the oxidative capacity of the muscles with greater aerobic potential in a manner similar to the performance of physical exercise. When exercise and treatment are combined, the effects that are obtained separately are not potentiated.

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A histochemical ATPase method for the demonstration of the muscle capillary network.

Cited by 22 publications

References 26 publications

Capillary supply, fibre types and fibre morphometry in rat tibialis anterior and diaphragm muscles after intermittent exposure to hypobaric hypoxia

Capillary supply, fibre types and fibre morphometry in rat tibialis anterior and diaphragm muscles after intermittent exposure to hypobaric hypoxia

Capillary Supply and Fiber Morphometry in Rat Myocardium after Intermittent Exposure to Hypobaric Hypoxia

Effects of a Standardized Panax ginseng Extract on the Skeletal Muscle of the Rat: A Comparative Study in Animals at Rest and Under Exercise

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