A histological comparison of the development of pollen and female gametophytes in fertile and sterile Cryptomeria japonica

Hosoo, Yoshihiro; Yoshii, Eri; Negishi, Kenji; Taira, Hideaki

doi:10.1007/s00497-005-0003-3

Cited by 28 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the male-sterile C. japonica trees Shindai1 and Shindai5, microsporogenesis proceeded normally and normal microspores were produced, but the microspores contracted and clumped by the time of pollen dispersal (Yoshii and Taira 2007). In Shindai2 and Shindai10 trees, which are both male-and female-sterile, pollen grains of various sizes were formed due to meiotic defects (Hosoo et al 2005;Kawana et al 2006). Therefore, the results of this study indicate that the mechanism causing male sterility in Shindai3 differs from that reported previously for other male-sterile trees of C. japonica.…”

Section: Discussionmentioning

confidence: 98%

“…In the male-sterile C. japonica trees Shindai1 and Shindai5, which were discovered in Niigata, Japan, microsporogenesis proceeded normally and normal microspores were produced, but the microspores contracted and clumped by the time of pollen dispersal (Yoshii and Taira 2007). In other C. japonica trees (i.e., Shindai2 and Shindai10), which are both male-and femalesterile, pollen grains of various sizes were formed due to meiotic defects (Hosoo et al 2005;Kawana et al 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytological study of a male-sterile Cryptomeria japonica that does not release microspores from tetrads

Ueuma

Yoshii²,

Hosoo

et al. 2009

Journal of Forest Research

Self Cite

View full text Add to dashboard Cite

To determine the mechanism of male-sterility Cryptomeria japonica tree Shindai3, the process of microspore development was observed under light and fluorescence microscopy. Microspore development in the Shindai3 was normal until the tetrad stage, but separation of the microspores from the tetrads was not observed even after callose had been degraded. In contrast to the microspore stage in a male-fertile tree, amorphous substances were observed around tetrads in the Shindai3, and the amount of the amorphous substance increased from the middle of November to the middle of December. The substances are hypothesized to prevent the separation of individual microspores from tetrads by inducing adhesion between microspore cells within the tetrad or enclosing the tetrads. The form of the tetrads in the Shindai3 was maintained until just before the pollen dispersal season, but they ultimately degenerated. The results of this study indicate that the mechanism causing male sterility in Shindai3 differs from that previously reported for other male-sterile trees of C. japonica.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Cytological study of a male-sterile Cryptomeria japonica that does not release microspores from tetrads

Ueuma

Yoshii²,

Hosoo

et al. 2009

Journal of Forest Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chen et al (2004) also observed similar phenomena in citrus allotetraploid somatic hybrids and speculated that these phenomena resulted in the production of sterile pollens. Moreover, Hosoo et al (2005) proposed that monads of Cryptomeria japonica (L. f.) D. Don was incited by nuclear fusion or defects in chromosome segregation. Formation of microspores from dyads to polyads seems to result from abnormal chromosome segregation and cytokinesis.…”

Section: Discussionmentioning

confidence: 99%

Abnormal Microspore Development Leads to Pollen Abortion in a Seedless Mutant of ‘Ougan’ Mandarin (Citrus suavissima Hort. ex Tanaka)

Hu¹,

Zhang²,

Wen³

et al. 2007

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Seedlessness is of commercial importance in citrus (Citrus L.). Seedless ‘Ougan’ mandarin (C. suavissima) was selected from a bud sport mutation that occurred in ‘Ougan’ mandarin. We analyzed their pollen viability through KI-I2 and FDA staining, and examined the anthers of wild-type (seedy) and seedless mutant ‘Ougan’ mandarin using histological and cytochemical methods to characterize the process of pollen development. No pollen fertility was detected in this mutant. Pollen abortion in anthers of the mutant occurred at the tetrad stage of microspore development, and almost all the tetrads were abnormal. The mutant had heterogeneous microspore populations, including monads, dyads, triads, tetrads, and polyads in the same microsporangium. Pollen grain number per anther of the mutant was 21.9% less than the wild type. Morphology of mature pollen grains using SEM showed that the shape of mature pollen grains from both wild type and mutant is similar, but the microsporangia of the latter contained pollen grains of more variable sizes. At the early mature pollen grain stage, abundant starch grains and lipids appeared in the wild type's pollen, but fewer amounts were observed in the mutant. Moreover, the tapetal cells of the wild type accumulated lipids, but not those of the mutant. Results indicated that the abnormal development of the microspore led to pollen abortion in the mutant, and this could be the reason for its seedlessness. However, the genetic reasons for the aberrant tetrads are not clear and are under investigation.

show abstract

“…Wheat is a self-pollinating crop [1,2] and is known to have a relatively high moisture content and a short life span [3].Therefore, pollination must be done within 30-40 minutes of pollen shedding in order to produce effective seed sets [4]. Pollen viability has a signi cant effect on fertilization [33], embryonic development [34] and quality of seed [35]. Plant pollen viability and longevity vary depending on species and environmental conditions [6].…”

Section: Discussionmentioning

confidence: 99%

A simple method for preserving pollen viability and longevity in wheat

Khan

Sajjad

2023

Preprint

View full text Add to dashboard Cite

Wheat pollen grains have very short longevity and are non-viable after ~30 minute at room temperature and ~60 minutes at 4°C. Pollen grain viability can be prolonged to ~24 hrs with existing anther preservation methods. Herein, we developed a new and very simple method of spike preservation to prolong pollen viability up to 1 week. The present study was evaluated to determine the effect of storage temperature and duration on the viability and in vitro germination of pollen grains of 50 spring wheat genotypes from two preservation methods of pollens viz, Anther preservation method and Spike preservation method. Pollens collected from both methods were stored at room temperature (22°C), fridge (4°C) for 0 and 1 week. Pollen viability were assessed using Alexander staining techniques with both methods at storage conditions 22°C and 4°C. In vitro germination was determined using liquid germination medium with both methods at 4°C. The results revealed that the pollen viability and in vitro germination as determined by Spike preservation method was highest when pollens were stored at 4°C up to 1 week. In addition, in vitro pollen germination and pollen viability significantly reduced as storage duration increased. It was recorded, that no pollen germination was observed under anther preservation method at storage conditions 4°C after 1 week while 2 to 14% pollen germination was obtained by spike preservation method only at 4°C up to 1 week. Based on these results, it is suggested that a storage temperature of 4°C by Spike preservation method for wheat pollen storage is good. The present study would be useful to develop a protocol for the storage of pollen for further use in the conservation and breeding of wheat.

show abstract

A histological comparison of the development of pollen and female gametophytes in fertile and sterile Cryptomeria japonica

Cited by 28 publications

References 31 publications

Cytological study of a male-sterile Cryptomeria japonica that does not release microspores from tetrads

Cytological study of a male-sterile Cryptomeria japonica that does not release microspores from tetrads

Abnormal Microspore Development Leads to Pollen Abortion in a Seedless Mutant of ‘Ougan’ Mandarin (Citrus suavissima Hort. ex Tanaka)

A simple method for preserving pollen viability and longevity in wheat

Contact Info

Product

Resources

About